... open-ing of the mPTP. There is evidence that ADP inhibitsopening of the mPTP by occupying binding siteslocated in the inner and outer mitochondrial mem-brane [39,40]. The binding of ADP stabilizes ... –Ca2+,15 min in incubation medium; Ca2+, 15 min in incubation mediumplus 3.5 lM Ca2+;H⁄ R, 10 min hypoxia followed by 5 min reoxy-genation; CSA, 2 lM cyclosporin A. A volume of 2 mL of the incu-bation ... metabolized providing the NADH-yielding substrate pyruvate. Under these con-ditions, some succinate is formed by the citric acidcycle, oxidizing malate, even in the case of the NADH-depending substrate...