... clusters [25], glucokinase activity increasedlinearly with increasing glucose concentration. All these®ndings indicate that the poor release of insulin by fetalpancreatic b cells in response to ... is acetyl-CoA hydrolaseactivity) from the change in A412 after addition of oxalace-tate.Succinate dehydrogenase activity [18] was determined after mixing 4 0 lL tissue homogenate with 500 ... for 5 min, and thesupernatants obtained were frozen at )80 °C until theinsulin assays. Insulin was measured by the radioimmun o-assay method of Herbert et al. [15], using rat insulin as astanda...