... H.H.O.,
Matsuda,S.,Kanemitsu,N.,Nakamura ,A. ,Mimura ,Y. ,Ueda,
N., Kurahashi, Y. & Yamamoto, S. (1997) Anandamide and other
N-acylethanolamines in mouse peritoneal macrophages. Meta-
bolism of anandamide, an endogenous cannabinoid ... 1.
MATERIALS AND METHODS
Materials and animals
Deuterated anandamide, PalEtn and 2-AG were synthe-
sized from [
2
H
4
]palmitic acid and [
2...
... NICN1,wehavenow
analyzed the human and murine orthologs and provide
initial data on the expression profiles and cellular localiza-
tion of the protein product.
MATERIALS AND METHODS
General methods
Standard ... human and a
murine NICN1 cDNA clone from the IMAGE collection
and determined the complete sequences of these cDNAs.
The comparative analysis of the hum...
... driven by a favorable entropy increase with
a less favorable enthalpy decrease, in line with a partial
intercalation mode involved in BLM-catalyzed breakage of
DNA.
Keywords: bleomycin; DNA cleavage; ... reaction
without taking self-inactivation of the enzyme into account.
It is a novel application of the thermo-analytical analog
curve method and suitable to both fast...
... connecting strand
b3 to helix a1 and two small h elices (a4 anda6).
The catalytic site consists of a functional catalytic triad
found in all serine enzymes of the a/ b hydrolase fold family
in which ... His290 and Asp260 e merge
from the b8 -a8 andb7 -a7 loops, respectively. Another
important feature of the catalytic machinery is the so-called
oxyanion hole necessa...
... of the
surfaces were determined using distances between ligand
atoms and protein atoms of less than 3 A
˚
. These additional
interactions are solely due to crystal packing and are
certainly contributing ... MUC-1; binding epitope.
The extracellular part of the epithelial glycoprotein MUC-1
consists of tandem repeats of 20 amino acids
(PDTRPAPGSTAPPAHGVTSA, where the...