... after 40 cycles
consisting of 1 min at 94 °C, 1 min at 44 °Cand1minat
72 °C in a Hybaid thermocycler. Subcloning in pZERO
(Invitrogen) using EcoRI and BamHI resulted in loss of a
part of the amplified ... be the start of the protein
coding domain. Sequence analysis of the 3¢ end cDNA
revealed that there is a polyadenylation signal upstream of
the poly(A).
Analysis...
... (YPPs)
by the fat body and their subsequent internalization
into the developing oocyte, helping to support later
embryonic development. The two primary insect
Keywords
ecdysone receptor; 20-hydroxyecdysone;
nuclear ... meal
(PBM), peaking at 18–24 h PBM [3]. 20E is one of the
primary regulators in the synthesis of vitellogenin (Vg),
the main YPP protein produced by the f...
... leads to the formation of
nicotine blue from the hydroxylated pyridine ring and of c-N-methyl-
aminobutyrate (CH
3
-4-aminobutyrate) from the pyrrolidine ring of the
molecule. Surprisingly, two ... Succinate may enter the citric acid
cycle completing the catabolism of the pyrrolidine moiety of nicotine.
Expression of the genes of these enzymes was dependent on...
... the effect produced
by the treatment with the proteasome inhibitors on cell
viability and G protein activity by analyzing the release
of inositol phosphates by carbachol in cells expressing
the ... rate with 60 and 40% of the pro-
tein remaining at 3 and 6 h, respectively, both for the
ligand-activated and nonactivated Ga
q
. Adding new
media containing 10 lm...
... isoforms of CytOX V [10,32]. The
regulation of genes CytOX5a and CytOX5b, coding for the
two isofoms Va and Vb, parallels that of genes CYC1 and
CYC7, which encode iso-1 and iso-2 of yeast cytochrome ... 1). With the accompanying reduction in heme aa
3
levels, the enzyme activity per mg SMP protein indicates a
change in the catalytic efficiency (TN) of the e...
... CPUs for bra-
dykinin and Hip-Arg when the kinetic assay was used,
proving that the mutations in the YQ proCPU did not
alter the affinity of the carboxypeptidase for synthetic
and physiological substrates. ... their
activity was determined (Table 2). These combinations
increased the stability of CPU’s activity, especially the
YQ mutant.
Following the first round of F...