Biochemical characterization of thermophilic lignocellulose degrading enzymes and their potential for biomass bioprocessing
Báo cáo khoa học: Crystal structure of RNase A tandem enzymes and their interaction with the cytosolic ribonuclease inhibitor potx
... structure of RNase A tandem enzymes and their interaction with the cytosolic ribonuclease inhibitorUlrich Arnold, Franziska Leich*, Piotr Neumann, Hauke Lilie and Renate Ulbrich-HofmannDepartment of ... crystal structures of three tandem enzymes withthe linker sequences GPPG, SGSGSG, and SGRSGRSG, which allowed usto analyze the mode of binding of RI to the RNase A tandem enzymes. Modeling studies ... catalytically active and that theactivity decrease [16] is a result of the tandemization.Accessibility of the linker of SGRSGRSG-RATE inthe presence of RIThe accessibility of the linker sequence...
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Báo cáo khoa học: Glycolysis in Entamoeba histolytica Biochemical characterization of recombinant glycolytic enzymes and flux control analysis ppt
... with ahigher concentration of PGAM than of PPDK atpH 6. The control analysis showed CJEivalues of 0.08 and 0.2 for PGAM and 0.85 and 0.57 for PPDK at 10 and 39 mU of added PPDK, respectively. ... instead of PPi-PFK and PYK instead of PPDK). At pH 6.0,the Vmaxvalues of amoebal PGAM and PPDKshowed a slight increase, those of HK, HPI, TPI and ENO were relatively unchanged, and those of PPi-PFK, ... at which amoebas grow in vitro and in the host, and at optimal pH and at pH values of 6.0 and 7.0.The Vmaxvalues of the His-tagged recombinant enzymes in the forward (glycolytic) direction...
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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx
... software swiss-pdbviewer and visual molecular dynamics [49].AcknowledgementsWe thank G. Magni and S. Ruggieri for helpful com-ments and suggestions and T. Begley (Department of Chemistry and ... inmedia containing isoform I. Both isoforms werealso expressed intracellularly, as evidenced by theappearance of protein bands of the expected sizeupon SDS ⁄ PAGE analysis of the crude extractsprepared ... amplification and cloning of ACMSDisoformsHuman brain, liver and kidney total RNA (ClontechLaboratories, Inc., Mountain View, CA, USA) was reversetranscribed in the presence of random primers,...
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Tài liệu Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera pptx
... jellyroll domain formed by two b-sheets and a distal domain formed by insertions between thestrands of the jellyroll domain (Fig. 3). Antiparallelb-sheets made up of strands CHEF and BIDG packagainst ... ionicstrength of the solvent environment and ⁄ or presence of small organic compounds (dioxane and MPD)seems to favor formation of dodecahedra over isolatedpentamers. Previous structural analysis of the ... A˚) of dodecahedral penton and penton baseparticles formed by the hAd3 serotype revealed addi-tional density in the region of the hypervariable loop,implying some favored conformations of the...
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Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx
... crotonyl-CoA,3-hydroxybutyryl-CoA and 3-methylcrotonyl-CoAwere used for the kinetic characterization of AUH.The data are summarized in Table 2.Overexpression of a mutant form of AUH and its activity on (E)-3-MG-1-CoAMutations ... proceduresProduction and purification of human AUHin E. coliThe production of AUHp40 (precursor form), AUHp32(mature form), AUHp40A240 V and AUHp32A240 V inE. coli was performed using the pMAL-c2 ... shoulder and the tailing is due to acetyl-CoA and not due toany other compound. (B) The peaks 3, 4, and 5 were isolated by HPLC and used as substrates for AUH. The AUH assay contained 2 mM of the...
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Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc
... large number of orthologs(Figs 2,9). This suggests an essential role for FMN despitethe low affinity of the enzyme for that cofactor and theapparent absence of a redox process as part of the catalyticcycle. ... homooctamerstructure with a relative mass of 309 kDa [29]. In order tocheck for the potential influence of substrates and cofactorson the quaternary structure of the enzyme, we performedboundary sedimentation ... exception of Mycoplasma gallisepticum R, Myco-plasma penetrans and Spiroplasma kunkelii, the genomes of the members of the mollicutes group (total number, 7 out of 10) are devoid of genes for the...
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Tài liệu Báo cáo Y học: Structural and biochemical characterization of neuronal calretinin domain I– II (residues 1– 100) Comparison to homologous calbindin D28k domain I–II (residues 1 –93) pdf
... calcium-binding loops of EF-hand proteins, have chemical shifts . 10 p.p.m. for filled loops and chemical shifts of < 8 p.p.m. for emptyloops [50]. The chemical shifts of CR I–II, Calb I – II and several ... University of Debrecen, Hungary;3Department of Inorganic Chemistry, and 4Research Group for Antibiotics, Department of Chemistry, University of Debrecen, Hungary;5International Institute of Molecular ... 2001)Abbreviations: CR, calretinin (rat isoform, unless otherwise stated); CRI–II, calretinin residues 1–100 consisting of the first pair of EF-handmotifs; Calb, calbindin D28k(rat isoform, unless otherwise...
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Báo cáo khoa học: Biochemical characterization of USP7 reveals post-translational modification sites and structural requirements for substrate processing and subcellular localization pptx
... process-ing of ubiquitin and ubiquitin-like proteins. They areresponsible for the disassembly of ubiquitin chains, and for the cleavage of mono- and oligomers of this mole-cule, either in precursor form ... Coomassie stained and major bands were excised and eluted in order to perform N-terminal sequencing.Tandem affinity purification and massspectrometric analysis of USP7N-terminal and C-terminal ... Institutes for Biomedical Research, Basel, Switzerland4 Biologics Centre, Novartis Institutes for Biomedical Research, Basel, SwitzerlandDeubiquitinating enzymes (DUBs) are a superfamily of thiol- and...
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Báo cáo khoa học: Molecular cloning and characterization of methylenedioxy bridge-forming enzymes involved in stylopine biosynthesis in Eschscholzia californica doc
... structures of isoquinolinealkaloids, because the structures of many P450s areclosely correlated with those of their substrates. Fur-ther identification and characterization of other mem-bers of the ... (R,S)-tetrahydroberberine.Amplification of cytochrome P450 cDNAfragments from single-stranded cDNAs of cultured E. californica cellsSingle-stranded cDNAs were synthesized from 1.4 lgoftotal RNA of 12-day-old cultured E. californica ... parameters of CYP719A3, the amount of nandinine produced was estima-ted by using the calibration curve of (S)-scoulerine (pmolversus peak area) and the absorption ratio of (S)-scoulerine and (S)-nandinine...
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Từ khóa: a comparison of international financial reporting standards and us gaap for investment fundsprocess of photosynthesis in green plants and its significance for other organismsecology is the study of the interaction between organisms and their environmentisolation and preparation of rna from rat blood and lymphatic microvessels for use in microarray analysispart three of the general accounting plan contains the standards for the preparation of annual accounts with standard and abbreviated models for the documents that comprise the annual accounts including the contents of the notespreparation of metal loaded porous carbons and their use as a highly active catalyst for reduction of nitric oxide noconstruction and management of a lying in institution and training school for midwives and midwifery nursesmolecular properties of choline acetyltransferase and their importance for the compartmentation of acetylcholine synthesis2the effects of inflammation on stem cells and their nichebiomechanical studies of primate locomotion and their implications for understanding primate neuroethologyhearings and their fallout for tax collectionnanomaterials conducting polymers and their nanocomposites for solar energysome trends and projections and their implications for world order w michael reisman and andrea armstronghigh density linkage maps in conifer species and their potential applicationepa methods and their limitations for oil analysisNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Phát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Sở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXTranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ