... bysite-directed PCR-based mutagenesis [12,30]. Mutagenicantisense oligonucleotides for MCAD 842GfiC(5¢-GATAAAACCACACCTGTAGTAGCTG-3¢) and MCAD116 5A G(5¢-CCTGTAGAAAGACTAATGAGGGATGCC-3¢) (mutagenic substitutions ... Ikeda Y, Okamura-Ikeda K & Tanaka K (1985) Purifi-cation and characterization of short-chain, medium-chain, and long-chain acyl-CoA dehydrogenases fromrat liver mitochondria: isolation of ... is clearly an acute mutation,because, regardless of its stability, the catalytic activitywas completely abolished, as determined by the ferrice-nium assay, ETF assay, and the INT ⁄ PES activity...