... acyl-transfer kinetics of the various mutants.The proposed model for transfer of the acyl chainfrom ACP to KAS III, a CHN group enzyme, consists of two steps: specific, albeit weak, binding of ... Among these is a large family of enzymes involved in synthesis of fatty acids, waxes,flavins, natural drugs, and antibiotics making carbon–carbon bonds by use of the Claisen condensation prin-ciple. ... [25]. The formation of the fatty acid complex is accompanied by the emergence of a well-defined water molecule within hydrogen bond distance of His333 Ne(Fig. 3A,B).Structures of KAS I H298E and...