... (Ambion Inc., Austin, TX, USA) [pool number M-007643– 00, sequences CCAAGGAGATTGAAGAATT (1), GAT CATGCACTGAAATTTA (2), GATGAAACCTCTCAA ACTG (3), and CATCATCGCTGGACAATGT (4)], using FEBS Journal ... lL of a substrate solution (Promega, Madison, WI, USA; CytoTox96 assay), and after 15 of incubation, an additional 50 lL of a stop solution Thereafter, extinction was measured at a wavelength of ... with an attenuated or even abolished increase in intracellular cAMP concentrations This demonstrates that the ionomycin-induced increase in intracellular Ca2+ concentration leads to activation of...