... (Roche Diagnostics, Meylan, France) and AAP determination was measured using storage method and colorimetric assay NAG and AAP activity (U/L) was expressed as a ratio with urinary creatinine (mmol/L) ... MA, Shenton BK, Balupuri S, Gupta AJ, Asher J, Talbot D: Weight increase during machine perfusion may be an indicator of organ and in particular, vascular damage Ann Transplant 2004, 9:31-32 ... (PNF) of the graft was defined as a total absence of urine output for consecutive days after transplantation and since dialysis is not available in our animal facility, animals with PNF were sacrificed...