... effects, and by allowing 300 s for association and 900 s for dissociation.In the case of IGF-I and related analogues, the concentra-tions used were 12.5, 25, 50, 100, and 200 nM,andforIGF -II and ... 1200BCFig. 2. BIAcore analysis of IGF -II and IGF -II analogue binding to high-anity recombinant IGF1R. Bin ding of IGF -II (A), d es-(1±6)-IGF -II (B) and Arg6-IGF -II (C) to rhIGF1R measured by BIAcore ... deprivation-induced cell death (Fig. 4E) and Arg6-IGF -II and des(1±6)-IGF -II were essentially equipotent toIGF -II. Leu27-IGF -II and d es-(1±6,10)-Leu27-IGF -II didnot exhibit any survival-promoting effects...