... Technology, Nakhon Ratchasima, Thailand2 National Synchrotron Research Center, Nakhon Ratchasima, Thailand3 Department of Biochemistry and Center for Protein Structure and Function, Faculty of Science, ... regression plots, theKm and kcatvalues determined for the spectroscopicassay were 1.04 ± 0.10 mm and 5.78 ± 0.58 s)1, and for the LC-MS assay were 1.05 ± 0.03 mm and 5.73 ± 0.16 s)1(Table ... blue showed single bands for thewild-type and mutants D392N and E315M, migratingwith an Mrof 63 000 (Fig. 4A). In the case of theE315Q mutant, an additional faint band was alsoseen at...