... of the L collinoides diol dehydratase The three genes pduCDE encoding the three subunits of the diol dehydratase were cloned in the pQE30 expression vector system The synthesis of the three subunits ... study, we report the purification, enzymatic characterization and analysis of the composition of the diol dehydratase of L collinoides...
... BA & Maki M (20 03) Analysis of epidermaltype transglutaminase (transglutaminase 3) in human stratified epithelia and cultured keratinocytes using monoclonal antibodies J Dermatol Sci 32 , 95–1 03 ... Baxa U & Steinert P (20 03) Roles of calcium ions in the activation and activity of the transglutaminase enzyme J Biol Chem 278, 238 34– 238 41 30 Hitomi K, Presland RB,...
... conditions a molecular mass of 125 000 Da was estimated for the liver enzyme and the serum enzyme and a molecular mass of 116 000 Da for the brain enzyme, indicating that all these enzymes exist as homodimers, ... Materials and methods TRH-degrading Lectin Brain enzyme Serum enzyme Liver enzyme SNA (Sambucus nigra A. ) GNA (Galanthus nivalis A. ) MAA...
... Mean and 95% confidence region of estimated standard deviations of the estimates for the cubic part of the Wiener-type model for a bandpass QAM -DMT signal with Nc = 6: optimal input signal via the ... performance of bandpass DMT signals Now that the optimal bandpass input signal for a linear FIR filter is found, the signal can be applied to the i...
... TCCACCACCCTGTTGCTGTA CCCTTTGCCATTGTTATA TCAGAGGTCGGTCAAACG ACGGGCAATACCCTCTGG AGGACTCGTCGGGAAGCA CCACCAGTCCTGATGCCA AGCCTTCAAGCGGGGAG CAAGCTCTTGAAAGGCATCG AGAGGGCTTCCTGGCTCA AACGCAGAGTACGCGGG CAGCCAAGGGTGGTCCTGC ... between Meishan · Large White and Large White · Meishan F1 hybrids and their parents ACL is a cytosolic enzyme that catalyses the formation of acetyl...
... aragonite crystals [4–7] We therefore examined proteins contained in aggregates within the otolith matrix and identified a protein contained in the HMW protein glycosaminoglycan aggregate that also ... digestion of genomic DNA contamination in the total RNA was confirmed by lack of amplification of a b-actin mRNA fragment by PCR using a pair of primers (5¢-ATCACCAT...
... identification and quantification of puromycin were achieved by a Pac enzymatic assay [21] Preparation of 3¢-amino-3¢-deoxyadenosine 3¢-amino-3¢-deoxyadenosine was obtained from Helminthosporium sp ATCC20154 ... Schematic representation of the putative biosynthetic pathway of the 3¢-amino-3¢deoxyadenosine moiety of A2 0 1A and puromycin Dpur4 mutants The results indica...
... Monteiro, M .A & Pang, H (1995) Lipo -oligosaccharide of Campylobacter lari type strain ATCC 35221 Structure of the liberated oligosaccharide and an associated extracellular polysaccharide Carbohydr ... Lipo -oligosaccharide of Campylobacter lari strain PC 637 Structure of the liberated oligosaccharide and an associated extracellular polysaccharide Carbohydr Res 279, 227– 2...
... using BSA as a < /b> standard according to the < /b> manufacturer’s protocol Any bacterial and protease contamination of < /b> the < /b> purified protein preparations was ruled out by the < /b> absence of < /b> bacterial DNA and the < /b> ... contamination of < /b> our protein preparations that might be responsible for activation of < /b> the < /b> PLB precursor at prolonged storag...
... papaya latex the primers 5¢-ACTCAAATCACTAGTATTCTTCCACCA-3¢ and 5¢-CATTTGAACATAAACATGAACAAATAAGTT-3¢ and the following conditions: annealing temperature 55 °C, 25 cycles, Phusion polymerase used according ... percentage of release from position was calculated as follows: area fatty acid ⁄ (area fatty acid + area lysoPtdCho) Pancreatic PLA2 was used as a positive control, and T lanugi...