... shoots having roots within 14 days. The most appropriate time for successful transplantation of in vitro plantlets to soil was from 12/1 to 22/2 with the rate of survival approaching 100% and ... for in vitro rapid propagation of Ligusticum wallichii Frach. using shoot tips. Using 0.15% aqueous mercuric chloride (HgCl2) solution for 10 min appeared to be optimal for surface disinfection ... trưởng, phát triển thu n lợi. Từ khóa: Ligusticum wallichii Frach, Xuyên khung, Nhân giống in vitro, MS, BA, IBA, Kinetin, a.NAA ABSTRACT Research was carried out in order to develop a protocol...