... and serves as an inhibitor for diol and glycerol dehydratases [57] Overall structure of glycerol dehydratase in complex with cobalamin and propane-1,2-diol The crystal structure of glycerol dehydratase ... dehydratase- bound cobalamins (2.9–5.1°) [9,10] Figure 4C indicates the comparison of the position of the a-acetamide side chain of pyrrol...
... using the SUMO-2/ pET2 8a as a template The PCR was carried out for 25 cycles of 30 s at 95 °C, 30 s at 55 °C and 30 s at 72 °C, using two primers 5¢-GGAATTCCATATGGGAGTCAAGACTGAGAA CAAC-3¢ and 5¢-CCGCTCGAGTCAACCTCCCGTCT ... determine a three-dimensional structure of human SUMO at high resolution by X-ray crystallography In this paper we present the crystal st...
... entire salinity range (Fig 2 .10 ) The activity profiles of AmyA at different temperatures and pH are shown in Figs 2 .11 and 2 . 12 Figure 2 .13 AmyA activity profile Activity assay of AmyA using 20 mM ... crystallographic statistics are summarized in Table 2 .1 40 Figure 2. 5 lAmyA crystal picture Crystals of AmyA, with maximum dimensions of 0 .1 x 0 .1 x 0...
... differences from the analogous structures of the ascomycete laccase (asco -laccase) MaL, thus giving a comprehensive view of the structure and function of asco-laccases Results and Discussion Overall structure ... Thornton JM (2003) Structural characterization and functional significance of transient protein–protein interactions J Mol Biol 325, 99 1–1 018 Crystal str...
... A2 for main-chain atoms, 35.7 A2 for side-chain ˚ overall (3 244 atoms) For the pepatoms and 33.8 A tide, B factors are slightly higher than those for ˚ ˚ importin -a: 36.9 A2 for main-chain atoms, ... atoms, 40 .2 A2 for ˚ overall (62 total atoms) side-chain atoms and 38.7 A Electron density analysis Both the main-chain and side-chain atoms of the modelled CLIC4 peptide...
... illustrates the superimposition of ApeSOD with the cambialistic SOD from P shermanii The overall structures of these enzymes were similar; the rmsd of the 747 Ca atoms was ˚ 0.695 A ApeSOD eluted from the ... superoxide dismutases Biochim Biophys Acta 1804, 24 5–2 62 Crystal structure of SOD from A pernix K1 Sugio S, Hiraoka BY & Yamakura F (2000) Crys...
... substrate recognition For the superposition of the substrate- free PhyK with substrate- free ˚ AppA the Ca atoms are 2.41 A apart, whereas for the substrate- free PhyK and the substrate- loaded AppA ... B Fig Crystal structure of Klebsiella sp ASR1 phytase (A) Cartoon representation showing the two domains of PhyK, the a domain (orange) and the a ⁄...
... 14-48 form the small insertion domain that comprises a short a- helix and a three-stranded anti-parallel b-sheet; the remaining protein residues form the (a ⁄ b)8 barrel domain and a C-terminal extension ... would act as a regulatory link between glycolysis and NAD synthesis The structure of hACMSD in complex with DHAP may used for the design o...
... N-terminal regions of the a-subunits serves as catalytic domains, and the C-terminal regions of the a-subunits and the b-subunits act as regulatory domains [10,11] The regulatory domains of AK contain ... ª 2009 The Authors Journal compilation ª 2009 FEBS 3131 Crystal structures of regulatory subunit of Thr-sensitive aspartate kinase Potential factors i...
... structure of the catalytic domain of DESC1 Scheme Domain organization of human DESC1 membrane region The extracellular part of DESC1 consists of a 120-amino acid SEA domain followed by the C-terminal ... growth factors and proteinase inhibitors Biol Chem 380, 473–483 Kataoka H, Uchino H, Asada Y, Hatakeyama K, Nabeshima K, Sumiyoshi A & Koono M (1997) Analy- C...
... References Ivanova N, Sorokin A, Anderson I, Galleron N, Candelon B, Kapatral V, Bhattacharyya A, Reznik G, Mikhailova N, Lapidus A et al (2003) Genome sequence of Bacillus cereus and comparative analysis ... of GAB catalysis have been identified to date [12] which are based either on a single, bifunctional GAB catalyst or on a GAB catalysts pair The available biochemical dat...
... protein crystallography Acta Crystallogr D Biol Crystallogr 50, 760–763 Navaza J (1994) Amore – an automated package for molecular replacement Acta Crystallogr A 50, 157–163 Jones TA, Zou JY, Cowan ... the accessible surface area of a monomer contributes to LlPDH dimer formation Whereas LlPDH and OaPDH have a ˚ buried surface area of 5500 A2 , TbPDH has a larger ˚ interface s...