... modification of the ribosome, the apparatus of translation, in terms of high-yield protein production Our development of this ribosomal protein mutation is one of the strategies to enhance protein ... highly productive in cell-free protein synthesis augmented with a dialysis system [23] The translation components, the reaction conditions, and the generation and the consumption of the energy source ... grown on plates containing various concentrations of streptomycin The sequence of the rpsL gene, which encodes the ribosomal protein S12, was confirmed by the PCR technique, and all mutations were...