... reproduction in ants, we investigated the fire ant VgR temporal subcellular localization in the ovaries of both virgin queens and mated queens, and attempted RNAi to silence the VgR in virgin queens ... al RNAi of vitellogenin receptor in fire ant queens Table Analysis of VgR silencing (RNAi) effect on ovaries from virgin queens at days 0, and 10 post eclosion Percentage of ovaries exhibiting oocytes ... nurturing work in the colony [26,28] In the fire ant, ovarian development and de-alating behavior in queens is correlated to the elevation of JH, RNAi of vitellogenin receptor in fire ant queens...