... and plasmid were analyzed using linearized pBAD ⁄ gIII plasmid, linearized and denatured plasmid and intact plasmid The pBAD ⁄ gIII plasmid was linearized using SalI and denatured by incubation ... Biochemical Adaptation Princeton University Press, Princeton, NJ Wu SI, Lo SK, Shao CP, Tsai HW & Hor LI (2001) Cloning and characterization of a periplasmic nuclease of Vibrio vulnificus and its ... constitutively expressed in V vulnificus [5] and Erwinia chrysanthemi [18] Endonuclease I from V salmonicida and V cholerae Here we report the cloning, expression and purification of the endonuclease I...