... cloned into pCRII vector and transformed into TOP10 cells (TOPO TA -cloning kit, Invitrogen) Sequencing reactions were performed using the ABI PRISM Big Dye Terminator cycle sequencing kit according ... of hybrid receptors in CHO-1 cells was performed to estimate the localization of receptors in cell detecting the EGFP in a fluorescent microscopy In 80–90% of the receptor EGFP fusion expressing ... these receptors is shown in Fig Maximum parsimony analysis of this group of receptors is shown in Fig 2, representing a consensus tree The dogfish MC3R sequence has highest Ó FEBS 2004 identity to...