... lumazine synthase /riboflavin synthase complex of Bacillus subtilis J Biol Chem 270, 16788–16795 Kis, K., Volk, R & Bacher, A (1995) Biosynthesis of riboflavin Studies on the reaction mechanism of 6,7-dimethyl-8-ribityllumazine ... binding of riboflavin together with small amounts of 6,7-dimethyl-8-ribityllumazine The situation is reminiscent of earlier observations by Plaut and coworkers who obtained riboflavin synthase ... the Kd value of 1.2 lM observed for riboflavin The optical spectrum of riboflavin bound to lumazine synthase from S pombe is characterized by a marked change in the relative intensities of the transition...