... interior of the protein matrix The script a is the peak–peak distance between the maximum at %287 nm and the minimum at %283 nm, and the script b is the peak–peak distance between the maximum at %295 ... are the experimentally determined numerical values of the ratio a/ b, and is the theoretical numerical value of ratio a/ b for a mixture of aromatic amino acids (Tyr and Trp), containing the same ... to a value of 8.0, by adding, alternatively, pH-unadjusted stock solutions of Tris and EDTA The final concentration of EDTA was mM Under these conditions, the pale blue of the supernatant at pH...