... dissociation constant The rate of clearance of the ternary T–AT–Fab complex in rats was only slightly slower than the rate of clearance of T–AT Studies with cultured HepG2 cells indicated that M27 ... stoichiometry of binding In vivo clearance of the T–AT complex in rats Thrombin, native AT, and elastase-cleaved AT were labeled with 125I (Amersham Pharmacia AB, Uppsala, Sweden) using the chloramine ... Although the Fab fragment of M27 caused a small, but significant (P < 0.0001, two-factorial ANOVA), reduction in the rate of clearance of the complex, from a half-life (t½) of in the absence of the...