... nonionic detergents: 0.05% (v/v) Nonidet P-40 or Tween-20 Enzyme substrates: 2,2'-azino-bis(3-ethylbenzathiazaline 6-sulfonic acid) (ABTS) (1 mg/mL, A405nm), O-phenyldiamine (OPD) (1 mg/mL, A492nm), ... chromogens that leave a colored precipitate on the blot (e.g., 3,3'-diaminobenzidine, 4-chloronapthol mix for HRP [14]; 5-bromo-4-chloro-3'-indolyphosphate toluidine salt, nitro blue tetrazolium chloride ... Histologically-based methods for detection of mucin, in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ Davies, R., Carlstedt, I (2000) Isolation of large gel-forming...