Proteomic analysis of saccharomyces cerevisiae KAY446 under very high gravity conditions
Báo cáo y học: "Comparative analysis of Saccharomyces cerevisiae WW domains and their interacting proteins" pps
... affinity (lower KDapp) WW WW WW WW (c) X WW WW Figure for protein ligands A model10 the optimization of interactions between WW domains and A model for the optimization of interactions between WW domains ... suggesting that WW domains have sufficient recognition malleability to bind structurally similar peptide ligands within the PY (PPxY) and LPxY ligand classes Both the PPxY and LPxY motifs were ... are Saccharomyces cerevisiae (Sc),Candida guilliermondii (Cgui),Candida glabrata (Cgla),Chaetomium globosum (Cglo),Kluyveromyces waltii (Kw),Kluyveromyces lactis (Kl),Yarrowia lipolytica (Yl),Candida...
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Báo cáo khoa học: Mutational analysis of functional domains in Mrs2p, the mitochondrial Mg2+ channel protein of Saccharomyces cerevisiae ppt
... either are involved in oligomerization of the Mrs2p channel protein or in forming structures participating in the gating of this channel Possibly they contribute to both functions The coiled-coil ... formation of higher order structures of the Mrs2p part on the inner side of the membrane, which may contribute to the proposed opening ⁄ closing of the channel The Mrs2 sequence C-terminal to the TM domains ... for the correct integration of the protein into the inner -mitochondrial membrane [3,8] Substitution of these glutamic acids by lysines (positively charged) resulted in a complete loss of mitochondrial...
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Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"
... molecules in the course of hypoxia-induced apoptotic pathways Such analysis of trophoblastic cells will, in the near future, yield insights into the mechanisms of preeclampsia and other hypoxia-related ... and by modulating phosphorylation of JNK In conclusion, various pro- and antiapoptosis-related proteins were expressed in the process of hypoxia-induced apoptosis in the trophoblastic cell line ... gene, but by modulating phosphorylation of JNKs Thus, Bag-1 may inhibit apoptosis by suppressing the expression of Bid and Bad Bag-1 may also enhance apoptosis by inhibiting the expression of Bcl-2...
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Performance analysis of wind turbine systems under different parameters effect
... Modeling of wind turbine The turbine is a wind energy conversion system It consists of an induction generator and other electrical control power requirements The wind turbine is effected by many parameters ... losses in wind turbine, ρ = air density in kg/m3, A = swept area in m2, Cp =power coefficient of wind turbine, V = wind speed in m/s Mechanical power of wind turbine is directly related to the wind ... 3550W at wind speed of 12m/s which is rated speed Table shows the standard classification system for wind turbines and effect of diameter of power rating of wind generating [8] The diameter of swept...
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Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx
... overexpressing a- synuclein A proteomic analysis is expected to identify cellular alterations that are associated with dopamine treatment and modulated by a- synuclein overexpression, without any a priori ... eukaryotic initiation factor 5A (eIF 5A) , parathymosin, L7 ⁄ L12, annexin A2 , annexin A5 , aldolase A, fascin and peroxyredoxin 1] displayed quantitative differences, regardless of whether or not a- synuclein ... above (Tables S2 and S3) In both cases, bioinformatic analysis revealed that the NF-jB pathway could be involved in determining the effects of dopamine treatment and a- synuclein overexpression Accordingly,...
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Tài liệu Báo cáo khoa học: Mammalian Gup1, a homolog of Saccharomyces cerevisiae glycerol uptake/transporter 1, acts as a negative regulator for N-terminal palmitoylation of Sonic hedgehog doc
... 5¢-CACACTACACTGGGAAGCAGAGACTCCAGC-3¢ and 5¢-AGCTGGCCCAGCAGCCATACACAGTTAAAG3¢ The cDNA was subcloned into the EcoRV site of pBluescript SK(+) (Stratagene, La Jolla, CA, USA) and sequenced using an automated ... Na -palmitoylation of Gas is regulated also remains unclear However, we assume that mammalian Gup1 competes with Skn for Shh to prevent palmitoylation rather than catalyzing depalmitoylation of ... buffer, and the eluted samples were subjected to western blot analysis as described above The authors thank Drs Masato Yasui, Sadakazu Aiso and Masaaki Matsuoka for support; Dr Andrew P McMahon for...
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Tài liệu Báo cáo khoa học: Integral membrane proteins in the mitochondrial outer membrane of Saccharomyces cerevisiae docx
... the most reliable means of purifying integral membrane proteins from the mitochondrial outer membrane, and of the 11 most abundant integral proteins, 10 had a-helical transmembrane segments Of ... the proteins of the outer membrane, we set out to determine what proportion was integral and to identify the major protein species To determine whether the major outer membrane proteins were integral ... assembly of beta-barrel membrane proteins J Biol Chem 276, 32399–32402 Integral proteins in the mitochondrial outer membrane Tokuda H & Matsuyama S (2004) Sorting of lipoproteins to the outer membrane...
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Tài liệu Báo cáo Y học: Targeting of malate synthase 1 to the peroxisomes of Saccharomyces cerevisiae cells depends on growth on oleic acid medium pptx
... Application of the antibody to thin sections of wild-type cells grown on oleic acid medium Fig SKL is required to direct Mls1p to the peroxisomes under oleic acid- medium conditions (A) Speci®city of the ... issue of the partitioning of the glyoxylate cycle in cells grown under fatty acid- medium conditions has hitherto remained open We showed here that one of the key glyoxylate-cycle enzymes, Mls1p, ... location of the glyoxylate cycle in yeast grown on carbon sources other than fatty acids The only other key enzyme unique to the glyoxylate cycle, Icl1p, is also extra-peroxisomal [4], as are the...
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Báo cáo khoa học: Energetic and metabolic transient response of Saccharomyces cerevisiae to benzoic acid docx
... properties and cell size to the presence of benzoic acid Transient response to benzoic acid total membrane surface area (=AXÆCXÆVL) available for the benzoic acid transport during the transient ... al Transient response to benzoic acid A B C D E Fig Transient responses to the shift in benzoic acid concentration (the timing of the shift is marked by a dashed vertical line) (A) Benzoic acid ... consumption due to the addition of benzoic acid In order to study the adaptation of cells to benzoic acid, we use the transient O2 consumption profile to reconstitute the dynamics in benzoic acid transport,...
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Báo cáo khoa học: Mutants of Saccharomyces cerevisiae deficient in acyl-CoA synthetases secrete fatty acids due to interrupted fatty acid recycling docx
... certain pools of fatty acids To assess the role of acyl-CoA synthetases in regulating particular pools of fatty acids, we used mutants of S cerevisiae characterized by various combinations of deletions ... exogenous fatty acids, additional release of internal fatty acids had to contribute to the increase of intracellular fatty acids However, analysis of the previously mentioned fatty acid specificity of ... (square)] (D) Concentration of fatty acids in the pools of free fatty acids in the media (diamonds), of free fatty acids in the cells (square) and of esterified fatty acids (triangle) The error...
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Báo cáo Y học: Expression of the V-ATPase proteolipid subunit of Acetabularia acetabulum in a VMA3-deficient strain of Saccharomyces cerevisiae and study of its complementation pdf
... AACEVAPD1 AACEVAPD3 AACEVAPD2 AACEVAPD5 AACEVAPD4 AACEVAPD6 Vma3p Vma11p Vma16p 100 97 80 80 80 80 50 50 32 100 94 93 93 54 51 28 AACEVAPD5 AACEVAPD2 AACEVAPD3 AACEVAPD1 Table Alignments of the ... R Hirata of the Institute of Physical and Chemical Research (Wako, Japan), and the antibody against the 100-kDa (a) subunit of S cerevisiae V-ATPase was purchased from Molecular Probes Inc., Eugene, ... preparation of recombinants in yeast expression vector In the case of AACEVAPD1, and 6, TAA is used as an Acetabularia- specific codon usage (translated as Gln) Conversion of TAA to CAA was performed by...
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Báo cáo khoa học: Lipins from plants are phosphatidate phosphatases that restore lipid synthesis in apah1Dmutant strain of Saccharomyces cerevisiae ppt
... PAHs are orthologs of yeast PAH1 and mammalian lipins Based on previous studies demonstrating the involvement of yeast PAH1 and mammalian lipins in storage lipid accumulation [27], we were initially ... derepression of key enzymes involved in phospholipid (PL) biosynthesis [20,21] In mammals, highly phosphorylated forms of Lipin-1 are enriched in the cytoplasm, whereas dephosphorylated forms are found ... homologs of PAH1 0.4 0.08 0.04 0.3 0.2 0.1 0.0 Fig Mutations within N-LIP and C-LIP domains in uence the ability of plant PAH1 to restore TAG synthesis in yeast pah1D strain (A) TAG ⁄ PL ratio of the...
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Báo cáo khoa học: Schemes of flux control in a model of Saccharomyces cerevisiae glycolysis docx
... investigate patterns of flux control The model with the best-fit Vmax parameters was used as the base model for parameter scanning using routines contained in GEPASI METHODS Model A model of branched glycolysis, ... in all combinations) using the parameter scanning functions of GEPASI Only around 50% of the simulations reached steady state, and of those that did a single step was usually seen to dominate flux ... of metabolite concentration and flux are obtained from populations of yeast cells and so reflect an aggregate of the states of many individual organisms Although no fitted model in this paper individually...
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Báo cáo khoa học: Activation of the plasma membrane H+-ATPase of Saccharomyces cerevisiae by glucose is mediated by dissociation of the H+-ATPase–acetylated tubulin complex docx
... al Dissociation of tubulin H+-ATPase complex by glucose H+-ATPase activity of isolated plasma membrane is inhibited by tubulin Plasma membranes isolated from S cerevisiae were incubated in the ... hydrophobic tubulin partitioning into the detergent phase (HAT) is the acetylated tubulin forming a complex with H+-ATPase The finding of the H+-ATPase acetylated tubulin complex in plasma membranes of glucose- starved ... some part of the H+-ATPase, reinforcing the idea of a complex between H+-ATPase and acetylated tubulin at the plasma membrane This complex was dissociated by pretreatment of cells with glucose...
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Từ khóa: succinate dehydrogenase of saccharomyces cerevisiae the unique enzyme of tca cycle current kanalysis of telecoms networkschemical analysis of materialssaccharomyces cerevisiae h2 rnaseanalysis of hull girderthe analysis of diatomfunctional analysis of the cervicalfunctional analysis of intergenicanalysis of the supremeanalysis of forcesnewtons lawsanalysis of an argumentanalysis of an issueanalysis of chemical warfare agentsanalysis of unknown wordsmorphosyntactic analysis of italiansyntactic analysis of swedishBáo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018Báo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọThơ nôm tứ tuyệt trào phúng hồ xuân hươngKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ