... cDNA molecules and sequence them GC(T)n Remove double-stranded cDNA/mRNA hybrids and mRNA molecules No PCR product GC(T)n TG(A)n AC(T)n PCR with primers specific to mRNA2 AC(T)n GC(T)n AC(A)n Hybridize ... location analysis with the nine known cell-cycle activators in yeast and showed that cell-cycle transcriptional control is a connected network For example, transcriptional regulators that act ... Thompson CM, Simon I, et al.: Transcriptional regulatory networks in Saccharomyces cerevisiae Science 2002, 298:799-804 21 Li Z, Van Calcar S, Qu C, Cavenee WK, Zhang MQ, Ren B: A global transcriptional...