... R, Qiao C & Mulchandani A (2010) Cotranslocation of methyl parathion hydrolase to the periplasm and of organophosphorus hydrolase to the cell surface of Escherichia coli by the Tat pathway and ... least three replicates The Km and kcat values were calculated by nonlinear regression using graphpad prism 5.0 (GraphPad Software Inc., La Jolla, CA, USA) Thermostability assay of WT and mutant ... thio-b-dgalactoside, kanamycin and imidazole were purchased from Ameresco (Tully, NY, USA) All chemicals were of analytical grade Construction of WT Ochr-MPH and mutants Genomic DNA of Ochrobactrum...