Insights into protein kinase a activation using cAMP analogs and amide h 2h exchange mass spectrometry

Tài liệu Báo cáo khoa học: Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes an increase in its hydrophobic surface area ppt

... that HSL gains accessible hydrophobic surface area upon PKA phosphorylation This gain in hydrophobic surface area presumably accounts for the increase in in vitro activity of HSL following PKA ... solvent-exposed hydrophobic surface area of HSL following PKA phosphorylation was examined using both bis-ANS and SYPRO Orange Because of the interaction of both bis-ANS and SYPRO Orange with ATP, we ... bis-ANS and SYPRO Orange after phosphorylation To investigate whether HSL gains hydrophobic surface area upon phosphorylation by PKA, we analysed the PKA phosphorylation of hormone-sensitive lipase...

Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf

... Bhagwat SV, Biswas G, Anandatheerthavarada HK, Addya S, Pandak W & Avadhani NG (199 9) Dual targeting property of the N-terminal signal sequence of P450 1A1 Targeting of heterologous proteins to ... open N-terminal signal domain is critical for protein targeting to the ER (Fig 2D) In contrast to the targeting of CYP 1A1 requiring N-terminal truncation, we have shown that intact CYP2B1 and CYP2E1 ... that xenobiotic-inducible CYPs such as rat CYP 1A1 , CYP2E1 and CYP2B1, and mouse CYP 1A1 , contain chimeric noncanonical -targeting signals that are capable of targeting proteins to both the ER and...

Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx

... nucleotide exchange factor with activity towards Ras Results HEK293 cells express the guanine nucleotide exchange factor Ras-GRF1 The guanine nucleotide exchange factor Ras-GRF1 is mainly expressed in ... physiological role of the guanine nucleotide exchange activity of the truncated forms is not known as they are missing the Ca2+ ⁄ CaM-binding IQ domain that is involved in the activation of Ras-GRF1 Stimulation ... isoforms of the guanine nucleotide exchange factor Ras-GRF1 in HEK293 cells Serotonin treatment of HEK293 cells, transiently transfected with the Gs-coupled 5-HT7 receptors, induced cAMP ⁄ PKA-dependent...

Báo cáo khóa học: SUT2 is a novel multicopy suppressor of low activity of the cAMP/protein kinase A pathway in yeast docx

... 5¢-TTCACGATTGAACAGGTAAACAAAATTTTCC CTTTTTAGAACGACATGCAGCTGAAGCTTCGTA CGC-3¢ and disRAS1rev CAAAACCATGTCATAT CAAGAGAGCAGGATCATTTTCAACAAATTATGC ATAGGCCACTAGGGATCTG-3¢ YEp351 -SUT2 was constructed to contain SUT2 as the ... yeast/ info/tools/hegemann/gfp.html) using the primers SUT-GFPfwd 5¢-GACTGTCGATGATTATGGTTGCC CGCTGGCTTCCAAACCCTTATCGATACCGTCGA CCC-3¢ and SUT-GFPrev 5¢-AACAATTTCACACACA GGAAACAGCTATGACCATGATTACGCTATAGG GCGAATTGGGTA-3¢, respectively ... The cassette was ampliﬁed from the plasmid pUG27 [13] using the primers disSUT2fwd 5¢-TGACGCTCACCAAGCTATTGGTTT GTTTGGATCAATCGTCAGATATGAAGGCATAG GCCACTAGTGGATCTG-3¢ and disSUT2rev 5¢-TAT TAATATTCCTATATTTTACATAGGAGGAAATTA...

Báo cáo khoa học: Inhibition of the NF-jB transcriptional activity by protein kinase A pot

... Madrid, L.V., Mayo, M.W., Reuther, J.Y & Baldwin, A. S Jr (2001) Akt stimulates the transactivation potential of the RelA/ p65 subunit of NF-kappa B through utilization of the Ikappa B kinase and ... PKA activation does not affect IjB degradation nor DNA-binding activity of NF-jB PKA impairs transactivation potential of p65 In the unstimulated cells, NF-jB is sequestrated in the cytoplasm as ... that the cAMP/ PKA signaling pathway inhibits transcriptional activity of NF-jB Although a previous report by others [22] suggested that PKA inhibited NF-jB activation by stabilizing IjB, PKA activating...

Báo cáo Y học: Electrostatic properties of the structure of the docking and dimerization domain of protein kinase A IIa doc

... pivot about the stable AKAP-bound dimerization and docking domain, and to weakly associate with another part of the molecule, possibly the tandem cAMP binding domains of the regulatory subunit ... secondary, tertiary, and quaternary structure packing and stability In addition, we discuss the role of charges in function of RIIa D/D MATERIALS AND METHODS Sample preparation and NMR spectroscopy ... N-terminal amide and His2 Aspartic acids Asp27 and Asp30 show lower mean pKa values than their model pKa values This may be a general property of Asp residues, as it has been observed in another...

Báo cáo khoa học: Recent insights into cerebral cavernous malformations: animal models of CCM and the human phenotype pptx

... function and lesion biology By exploring the function of the CCM genes in animal models this gap is being CCM animal models bridged Animal models have demonstrated the central importance of endothelial ... increase in both the intracerebral hemorrhage phenotype of rap1b and the cardiac developmental phenotype of san The zebraﬁsh experiments demonstrate the role of the CCM genes in cardiac and vascular ... further understand the human phenotype Recent investigations have further reﬁned our understanding of this phenotype, and bear reviewing in this manuscript In human CCM disease, the lesions exhibit...

Báo cáo khoa học: Malonyl-CoA decarboxylase (MCD) is differentially regulated in subcellular compartments by 5¢AMP-activated protein kinase (AMPK) Studies using H9c2 cells overexpressing MCD and AMPK by adenoviral gene transfer technique potx

... examine the role of AMPK in the regulation of MCD MCD activity in H9c2 cells coinfected with Ad.CA -AMPK and Ad .MCD Infection of H9c2 cells with Ad .MCD resulted in a signiﬁcant increase in MCD protein ... Ad.CAAMPK cells, P ¼ 0.058; Fig 2Biv) MCD expression and activity in subcellular fractions of H9c2 cells overexpressing both MCD and AMPK Fig AMPK overexpression by adenoviral gene transfer and endogenous ... overexpression and activity in H9c2 cells co-expressing Ad.CA -AMPK or Ad.GFP (A) MCD expression (i) and activity (ii) in H9c2 cells infected with Ad .MCD or Ad.GFP In the Western blot, lanes and ¼ Ad.GFP and...

Báo cáo Y học: The Emery–Dreifuss muscular dystrophy associated-protein emerin is phosphorylated on serine 49 by protein kinase A pptx

... respectively Phosphorylation was conﬁrmed by analysis of the recombinant emerin by electrospray ionisation mass Fig Baculovirus expressed 1–176 emerin analyzed by ESI Q-TOF mass spectrometry (A) The raw ... (ONJ) ONJ is a Lundbeck Foundation Research Professor 12 13 References Nagano A, Koga R, Ogawa M, Kurano Y, Kawada J, Okada R, Hayashi YK, Tsukahara T & Arahata K (1996) Emerin deﬁciency at the ... 2271.7 and 2396.9 The y axis is an arbitrary scale and represents relative intensity to be emerin as ascertained by molecular mass determination by MALDI-MS and amino acid sequencing by ESI tandem...

Báo cáo khoa học: Inactive forms of the catalytic subunit of protein kinase A are expressed in the brain of higher primates potx

... TGCCATGAAGATCTTAGA TGAGCAGTACTACGCCATGA GTAGCCCTGCTGGTCAATGA TTCCGTAGAAGGTCCTTGAG (VII) TTCCGTAGAAGGTCCTTGAG (VII) CCTAATGCCCACCAATCCA (VI) TTCCGTAGAAGGTCCTTGAG (VII) TTCCGTAGAAGGTCCTTGAG (VII) CTAATCTATGAAATGGCAG ... band seen in NT2-N cells is present in brain, but not in PBL (Fig 3A, lanes and 3) To examine whether the CbD4 variants were expressed in different parts of the brain as well as in fetal brain, ... was carried out using the Cb common primer pair on cDNA from hippocampus, amygdala and cerebral cortex of human adult brain, and on cDNA from human fetal brain Cb was barely detectable in fetal...

Báo cáo khoa học: Downregulation of protease-activated receptor-1 in human lung ﬁbroblasts is speciﬁcally mediated by the prostaglandin E2 receptor EP2 through cAMP elevation and protein kinase A pot

... cyclooxygenase pathway PGE2 is the major prostanoid synthesized by lung fibroblasts [11] It can also act on fibroblasts in a paracrine fashion after release from the adjacent epithelial layer [12] In addition ... differential modulation of the translation rate or the rate of internalization and degradation of PAR-1 protein As we and others [15–17,26,33] have shown the role of cAMP in the suppression of fibroblast ... fibrotic gingiva: interactions between cAMP and MAPK signaling pathways, and prostaglandin E2- EP3 receptor mediated activation of the c-JUN N-terminal kinase J Biol Chem 282, 15416–15429 27 Kamio...

Báo cáo Y học: The regulatory subunit of a cGMP-regulated protein kinase A of Trypanosoma brucei docx

... consistently coprecipitated a protein kinase activity exhibiting many characteristics of the catalytic subunit of trypanosomal PKA The coprecipitated kinase is recognized by an antibody against the ... demonstrated the presence of a PKA-like kinase activity in T cruzi [29] The current study describes the identiﬁcation and characterization of the regulatory subunit of trypanosomal PKA (TbRSU) Many of ... antibody against the catalytic subunit of bovine PKA In these experiments, the antibody detected a protein with a Mr of about 40 000, suggesting that the catalytic subunit of trypanosomal PKA does...

Báo cáo y học: " Protein kinase A-dependent Neuronal Nitric Oxide Synthase Activation Mediates the Enhancement of Baroreflex Response by Adrenomedullin in the Nucleus Tractus Solitarii of Rats" pps

... article as: Yen et al.: Protein kinase A-dependent Neuronal Nitric Oxide Synthase Activation Mediates the Enhancement of Baroreflex Response by Adrenomedullin in the Nucleus Tractus Solitarii of Rats ... adenylate cyclase; ADM: adrenomedullin; ADMR: adrenomedullin receptor; GC: guanylate cyclase; Gs: stimulatory GTP-binding protein; nNOS: neuronal nitric oxide synthase; PKA: protein kinase A; PKG: protein ... Chan JYH: Nitric oxide regulates c-fos expression in nucleus tractus solitarii induced by baroreceptor activation via cGMP-dependent protein kinase and cAMP response elementbinding protein phosphorylation...

báo cáo khoa học: " Construction of a potato consensus map and QTL meta-analysis offer new insights into the genetic architecture of late blight resistance and plant maturity traits" pps

... this article as: Danan et al.: Construction of a potato consensus map and QTL meta-analysis offer new insights into the genetic architecture of late blight resistance and plant maturity traits ... were called late blight meta-QTLs”, and meta-QTLs of maturity, vigour and plant height were called maturity meta-QTLs” Additional material Additional file 1: Description of the consensus map with ... chromosome The name, map position and occurrence of each marker are given in Additional file and on the SGN database [37] QTL dataset for meta-analysis Table Number of publications, maps and QTLs collected...

Insights into protein kinase a activation using cAMP analogs and amide h 2h exchange mass spectrometry

... Insights into Protein Kinase A Activation using cAMP Analogs and Amide H/ 2H Exchange Mass Spectrometry TANUSHREE BISHNOI A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE DEPARTMENT ... was coupled with 8-AEA -cAMP through a spacer arm A bed volume of ml of the resin was taken in a 50 ml tube The resin was reactivated by three alternative washes with High pH Buffer (200mM ethanolamine, ... tested, however Rp-cAMPS (Rp-adenosine 3’,5’-cyclic monophosphorothioate) and related derivatives are the only known cAMP analogues that act as antagonists and competitive inhibitors of cAMP mediated...

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