Study of the effect of transforming growth factor 1 on the gap junction protein connexin 43 in hepatic stellate cells

... RT-PCR was performed as described previously [7] SeP and GAPDH transcripts were coamplified using gene-specific primers Quantitative analyses were performed using a phosphorimaging system and QUANTITY ... Takahashi, K (1999) Selenoprotein P in human plasma as an extracellular phospholipid hydroperoxide glutathione peroxidase Isolation and enzymatic characterization of human selenoprotein P J Biol Chem ... added to give a total plasmid concentration of mg per well The figures in the bottom row are means of two independent replicates and represent densitometric values normalized to the intensity of the...

... in clinical settings [1] The definition of "benignity" concerning FL is wideaccepted [2] but conceptually difficult to maintain because the mechanisms, i.e., insulin resistance (IR), underlying ... look into the supposed "benignity" of FL and "progressivity" of NASH is to speculate about eventual differences/similarities in mechanisms between the two entities With this in mind, we tracked in ... up-regulation of transforming growth factor-beta1 (TGF-β1) [3] An alternative pattern is followed by leptin that facilitates proliferation and prevents apoptosis of HSCs [4] There is an increasing body of...

... mice Effect of anti-inflammatory drugs on glia activation and vascular amyloid deposition Ibuprofen or pioglitazone treatments were started at months of age and continued for months Two reasons ... anti-inflammatory drugs started at months of age, soon after weaning, and was carried out for months Ibuprofen was purchased from Sigma, and pioglitazone, manufactured by Takeda Pharmaceuticals, was obtained ... with an array of antibodies raised against human and rat A proteins that apparently stained vessels in a pattern identical to that obtained with thioflavin-S [6] At variance with this claim, FCA40...

... postoperative day to day Vessels were first noted on postoperative day As progressed, the Angiogenesis effects of nerve growth factor (NGF) on rat corneas 129 Fig Appearance of angiogenesis on day ... 1.0 ng of NGF group (Group 1.0), and the 5.0 ng of NGF group (Group 5.0) Data analysis The significant differences between groups were Angiogenesis effects of nerve growth factor (NGF) on rat corneas ... w/v) at 37oC with continuous stirring for 24 hours An equal volume of Hydron and sucralfate (12% w/v, Sigma Co, USA) were combined Also each concentration of nerve growth factor (NGF), such as 0.5...

... [25,29], gonadotrophin [15] or steroid hormone [15] The present study investigated the effect of βME or EGF supplementation on the base of the stages of the estrus cycle of the ovaries, and demonstrated ... was only observed in the oocytes collected from the follicular stage In conclusion, supplementation of canine IVM medium with GSH-synthesis stimulator, β- ME or EGF improved IVM of canine oocytes ... oocytes collected from the Effect of β- mercaptoethanol or epidermal growth factor supplementation on canine oocytes follicular stage, consistent with the result from Takahashi et al [32] in bovine...

... Hanly JG, Isenberg DA, Kalunian K, Nived O, et al.: The reliability of the Systemic Lupus International Collaborating Clinics/American College of Rheumatology Damage Index in patients with systemic ... antibodies and nine (28%) had lupus anticoagulant TGF-β1 activation index: an in vitro measure of the in vivo efficiency of TGF-β1 activation To examine the ability of SLE patients and control individuals ... TGF-β1, a ratio of levels of TGF-β1 in freshly collected blood and after 90 minutes of clotting at 37°C was determined The degranulation of platelets during clotting results in release of excess latent...

... significance The pre-defined pathways contained two variants of the TGFβ pathway (called 'TGF_Beta_Signaling _Pathway' and 'tgfbPathway') Both pathways were among the top 30 ranking pathways (out of 259) ... potency for further activation by the respective cytokines or growth factors, for example, transforming growth factor (TGF)β To enhance the significance of the array analysis, the mRNA data of the ... recruits the TGF-β-signal-transducing smads to the membrane in the close vicinity of the receptor [19] (Figure 1) This interesting finding provides detailed analysis of individual components of the...

... presence of FGF2 In addition, the chemical pathways utilized by FGF2 to antagonize the activity of BMP7 were analyzed to gain a better understanding of the complex interplay of growth factors, cytokines, ... activation of the ERK mitogenactivated protein kinase and NF-κB pathways are involved in the inhibitory action of FGF2 on BMP7 signaling via activation of noggin Discussion The present study demonstrates ... accumulation was mediated by an FGF2-mediated inhibition of PG synthesis, the incorporation of [35S]-sulfate by NP and AF cells into PGs was quantified The results showed that PG synthesis by both...

... Coulter Inc., Fullerton, CA, USA) according to manufacturer instructions Multiplex PCR and primer extension analysis of mutations in EGFR-216 promoter region and exons 18, 19, 20, and 21 Multiplex PCR ... amount of work and time required With this method, -216 promoter region and exons 18-21 of the EGFR gene were amplified with multiplex PCR in a single tube and the detection of mutations in the EGFR ... Feng Z, Roth JA, Herz J, Minna JD, Gazdar AF: Clinical and biological features associated with epidermal growth factor receptor gene mutations in lung cancers J Natl Cancer Inst 2005, 97(5):339-346...

... wild type group These data determine the dose response nature of lung injury following exposure to bleomycin Lung fibrosis induced by 4500 IU bleomycin in the Trwild type group was a mild patchy ... (Tr+ and bleomycin vs Tr- WT and bleomycin; Tr+ and bleomycin vs Tr+; Tr+ vs Tr- WT and bleomycin) A high number of altered genes were found to be upregulated and dowwnregulated in bleomycin treated ... tissue following exposure to bleomycin Conclusion De novo collagen production was assessed using hydroxyproline assay The lung hydroxyproline content was higher in the Tr+ transgenic-bleomycin...

... TGF-β1 in the urine of male rats after CYP were also significantly higher than the levels of active and total TGF-β1 in the urine of female rats, both at baseline and after CYP (ANOVA, Tukey post ... control rats collected at the same time point of the day The levels of NO2-/NO3- in the urine of CYP-treated female rats remained higher as compared to both CYP-treated and control male rats (ANOVA, ... (panel A) was maintained across all groups The substantial levels of latent TGF-β1 in female rats at baseline and after CYP was accompanied by only minor levels of active TGF-β1 in bladder tissue...

... M Jr, Palioto DB: Effects of enamel matrix derivative and transforming growth factor-beta1 on human periodontal ligament fibroblasts J Clin Periodontol 2007, 34:514-522 Page of 14 Tokiyasu Y, ... et al.: Effects of enamel matrix derivative and transforming growth factor-b1 on human osteoblastic cells Head & Face Medicine 2011 7:13 Submit your next manuscript to BioMed Central and take ... fetal enamel matrix derivative stimulates proliferation but not differentiation of pre -osteoblastic 2T9 cells, inhibits proliferation and stimulates differentiation of osteoblastlike MG63 cells, and...

... These indicate that there is an increased amount of TGF expressed in the airways that could be involved in the airway wall remodelling of chronic cough This is supported by the findings of a ... activity Sections were incubated with the avidin/biotinylated peroxidase complex for 30 min, followed by chromogenic substrate diaminobenzidine for min, and then counterstained in haematoxylin and ... Nature of airway inflammation and remodeling in chronic cough J Allergy Clin Immunol 2005, 116(3):565-70 McGarvey LP, Forsythe P, Heaney LG, MacMahon J, Ennis M: Bronchoalveolar lavage findings in...

... Cells during Liver Injury 1. 3 Transforming Growth Factor- 1 (TGF- 1) 1. 4 Gap Junction Protein - Connexin 43 1. 4 .1 Family of Connexins 1. 4.2 Connexin 43 in Hepatic Stellate Cells 1. 5 Zinc Finger ... of connexins into gap junctions The connexin protein consists of an amino acid chain containing transmembrane helices with the amino and carboxy terminus located intracellularly (A) Through interactions ... CHAPTER 1 INTRODUCTION 1. 1 Liver Fibrosis 1. 2 Hepatic Stellate Cells 1. 2 .1 Characteristics of the Hepatic Stellate Cells and their Functions in Normal Liver 1. 2.2 Activation of Hepatic Stellate Cells...