ELUCIDATING THE ROLE OF BNIP 2 IN ZEBRAFISH EARLY DEVELOPMENT

... (>0.3µM) inhibits the opening of InsP3Rs Thus, during the onset of InsP3Rs opening, the release of Ca2+ increases the sensitivity of InsP3Rs, resulting in a rapid rise in Ca2+ levels Once the Ca2+ ... to the low-affinity sites of calcineurin B and affects the conformation change of both CnB and the regulatory domain of CnA, resulting in the exposure of the calmodulin-binding domain (Sikkink ... al., 199 5) The Ca2+/calmodulin then bind to the CaM-binding domain and causes further conformational changes The conformation change in the flexible CaM-binding domain displaces the auto-inhibitory...

... transgenic line Establishing the role of autophagy in liver cancer 4.1 Preliminary data implicating autophagy inhibition in liver cancer 4.2 Establishing double transgenic lines with liver- specific ... as a model organism in the study of human diseases including cancers, we aim to investigate the role of autophagy in liver cancer using our established zebrafish liver cancer models As little study ... ELUCIDATING THE ROLE OF AUTOPHAGY IN ZEBRAFISH MODELS OF LIVER CANCER SIM HUEY FEN, TINA (B.Sc.(Hons.)), NUS A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE DEPARTMENT OF BIOLOGICAL...

... of glycogen in the intact liver in vivo Second, during infusion of [1-1 3C ]glucose, label incorporation was observed not only into the C1 of glycogen but also the C6 , which can only occur by label ... a mechanism can lead to increases in labeling of glycogen C6 even in the presence of decreased activity of the indirect pathway, provided that the increase in glycolytic flux exceeded the decreased ... (A) The stack plot of 1 3C spectra acquired over 7.6 h after beginning at t ¼ (right scale), the infusion of [1-1 3C ]glucose 1 3C- label incorporation into hepatic glycogen was detected in the glycogen...

... to the solvent, thus allowing for the binding of larger substituents (Fig 2A) The CH atoms of the substrate ring at positions and point towards the backbone of Leu248 and the side chain of Thr249, ... shown) The contacts between the edge of the dihydroxylated substrate ring and the active site pocket are probably involved in the determination of substrate specificity Inspection of the substrate ... surrounding the binding pocket The two complexes were very similar In both structures, the largest contacts were found between the plane of the substrate ring and the plane of the imidazole ring of residue...

... article as: Hughes-Fulford and Li: The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization Journal of Orthopaedic Surgery and Research 2011 6:8 Submit ... Figure FGF-2 and BMP-2, the yin and yang of mineralization: Contrast of effect of 24 hours of treatment with FGF-2 or BMP2 on fold increase in abundance of mineralization-related gene expression Mineralizing ... maintained the mineralizing RNA profile of igf-1, alp, and bmp-2 and significantly increased expression of other genes associated with mineralization like col1a1, fn, ilgf-1, noggin and oc Fgf-2, ...

... article as: Hughes-Fulford and Li: The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization Journal of Orthopaedic Surgery and Research 2011 6:8 Submit ... Figure FGF-2 and BMP-2, the yin and yang of mineralization: Contrast of effect of 24 hours of treatment with FGF-2 or BMP2 on fold increase in abundance of mineralization-related gene expression Mineralizing ... maintained the mineralizing RNA profile of igf-1, alp, and bmp-2 and significantly increased expression of other genes associated with mineralization like col1a1, fn, ilgf-1, noggin and oc Fgf-2, ...

... Leader peptide domain 5'-CAACACUCGCAAUAUU-3'(sense) 3'-GUUGUGAGCGACGUUAUAA-5'(antisense) α3 domain 5'-AGGUCUUAUGGUGCUGUCAUU-3'(sense) 3'-UUUCCAGAAUACCACGACAGU5'(antisense) Transmembrane domain ... Qa-2 domains for 24 hrs, and the expression of Qa-2 was then determined by reverse transcriptase-PCR and FACS analysis Lanes 4, and (α3 domain, transmembrane domain, and cytoplasmic domain, respectively) ... and siRNA The Qa-2 siRNA was mixed with α3 domain, transmembrane domain and cytoplasmic domain in equal amounts, after which it was administered to mice Flow cytometry To analyze the Qa-2 expression,...

... unwavering support of my goals iv ABSTRACT Sara M McNeil ELUCIDATING THE ROLE OF REDOX EFFECTS AND THE KU8 0 CTERMINAL REGION IN THE REGULATION OF THE HUMAN DNA REPAIR PROTEIN KU DNA double strand breaks ... than that of the 16 kDa Ku8 0CTR band and the band intensity generated from Ku7 0 and Ku8 0 C antibodies, indicating a small percentage of Ku8 0CTR incorporation into the Ku7 0/80C complex These data ... Preliminary work was performed to determine the elution volume of Ku7 0/80C and Ku8 0CTR independent of the other protein Individual proteins were pre-incubated in running buffer and applied to the...

... play a role in determining the pathogenicity of the PCV2 infection (Ramamoorthy et al., 20 09) This thesis describes the role of ORF3 in the pathogenesis of the PCV2 virus infection at the systemic ... the role of ORF3 in the pathogenesis of the virus infection in SPF piglets 28 2. 2 Materials and methods 2. 2.1 Viruses and cell culture The PCV2 were grown in PK-15 C1 cell line of porcine origin ... growth kinetics of PCV2 108 Figure 26 Role of ORF3 in the release of PCV2 from infected cells……… …111 Figure 27 Role of ORF3 induced apoptosis on the In Vivo spread of PCV… 113 Figure 28 Role of Macrophages...

... plasma cells in the < /b> bone marrow of < /b> apoE-/- mice Because we maintained the < /b> mice on BrdU in drinking water for one month, the < /b> result is also indicative that these plasma cells accumulated in the < /b> bone ... and/or increased bioavailability of < /b> 7α, 25 -OHC 3.4.1 Increased ch25h mRNA expression in the < /b> spleen of < /b> apoE-/- mice We examined the < /b> mRNA expression of < /b> ebi2, ch25h, cyp 7b1 and hsd 3b7 relative to the < /b> ... plasmablasts B1 cells also exists in the < /b> spleen but constituted only 1 -2% of < /b> CD19+ B cell population (Baumgarth, 20 11) Therefore, it remained possible that B1 a cells increased in the < /b> spleen of < /b> apoE-/-...