Use of lactococcus lactis as a mucosal vaccine delivery vehicle

... DEVELOPMENT OF BORDETELLA PERTUSSIS AS A LIVE VEHICLE FOR HETEROLOGOUS ANTIGEN DELIVERY, AND ITS APPLICATION AS A UNIVERSAL INFLUENZA A VACCINE LI RUI B.Sc.; M.Sc A THESIS SUBMITTED FOR THE ... is particularly well adapted for the nasal delivery of heterologous vaccines candidates and has already been reported as a promising mucosal vaccine delivery system Recently, a highly attenuated ... approximately million deaths The recent spread of HPAI H5N1 virus across Asia and parts of Europe and the Middle East, as well as the occasional infections of humans with an overall fatality rate of...

... stability of PANs was studied in the aqueous medium, and the acute toxicity of PANs was evaluated in mice Morever, EPI was loaded into PANs and its pharmacokinetics was also assessed in rats to compare ... main pharmacokinetic parameters were calculated by DAS 1.0 (Anhui, China) program Bioavailability (BA) is a measurement of the rate and extent of a therapeutically active drug that reaches the ... B A where AUC is the area under the curve Statistical analysis All data are presented as a mean value with its standard deviation indicated (mean ± SD) Statistical analysis was conducted using...

... in artificial tidal flats in Japan, a growth test of R philippinarum was also carried out in DS mixtures MATERIALS AND METHODS Artificial tidal flats in real seashore Five artificial tidal flats ... observed variation in the macrobenthos population in the artificial tidal flats and the natural tidal flat The abundance of macrobenthos in the artificial tidal flats increased after May 28 and finally ... Water and Environment Technology, Vol 7, No 3, 2009 Fig Location of the artificial and natural tidal flats in Tategami, Ago bay, Mie, Japan Table Sediment of artificial tidal flats in real seashore...

... And such a whole suite of accessory enzymes is required for efficient xylan hydrolysis such as α-arabinofuranosidases and α-Larabinases that release arabinan [31], α-glucuronidases that release ... pretreatment, enzymatic saccharification and fermentation of wheat straw to ethanol Journal of Biobased Materials and Bioenergy 2008, 2(3), 210-217 [68] Saha B.C., Cotta M .A Enzymatic hydrolysis and ... feasible as great amounts of this valuable commodity would be wasted Besides lignin, miscellaneous components of wheat straw such as wax, pectin, and phenolic acids are also of great value and...

... Expression of AcmA derivatives A1, A2, A3 and A4 in the L lactis NZ9000 mutants acmAD1 and acmAD1 DhtrA, visualized by zymographic analysis of culture supernatants of cells expressing the AcmA variants ... Acknowledgements Isolation of AcmA from the supernatant of L lactis cultures The cells of 100 mL of L lactis MG1363acmAD1 were boiled in 20 mL of 10% SDS for 15 min, washed six times with water and subsequently ... peptidoglycan-binding proteins were isolated from the supernatant of L lactis MG1363acmAD1 Determination of the hydrolytic activity of AcmA using RP-HPLC analysis B subtilis peptidoglycan was isolated...

... storage water 27 Use of solar process heat - REH, Brussels_130315 GEA Brewery Systems Heating of mash vessels With solar heated hot water / Installation of templates 28 Use of solar process heat ... Brussels_130315 GEA Brewery Systems Heating of mash vessels With solar heated hot water • Installation of a mash vessel with hot • • • • • 26 Use of solar process heat - REH, Brussels_130315 water heating ... wort heating with use of new process technologies Solar thermal energy / process application mashing • Heating of mashing process • Mash homogeneity • Design of mash kettle • Design of agitator Integration...

... Searching for a Mate: The Rise of the Internet as a Social Intermediary Abstract This paper explores how the efficiency of Internet search is changing the way Americans find romantic partners ... geographically concentrated in areas such as Silicon Valley, California, because the face-to-face networks are crucial for the cross fertilization of ideas Rosenfeld and Thomas, Searching for a Mate ... efficiency of search) for samesex couples, but the heterosexual partnership rate has been flat Searching for a Mate: The Rise of the Internet as a Social Intermediary Introduction: One under- appreciated...

... implies that MYP has a role as a zinc transporter for gametogenesis In vertebrates, vitellogenin, a precursor of yolk protein, is a zinc- bind4994 ing protein that transports the zinc required for oogenesis ... as a substrate for localization of the labeled MYP Statistical analysis Data were expressed as the mean ± SEM Statistical analysis was performed using instat software (GraphPad Software) The ... Zinc- binding protein in the sea urchin T Unuma et al mainly in the digestive tract [8] and in the nutritive phagocytes of the ovary and testis [10], and it is accumulated abundantly in the...

... features of aging A number of early explanations of aging, such as Orgel’s error catastrophe theory and the somatic mutation theory, were based on the idea that aging results from the accumulation ... neonatal rat cardiac myocytes, inhibition of autophagocytosis by 3-methyladenine results in a dramatic accumulation of small mitochondria while the number of large ones increases only slightly (A ... than normal mitochondria, or that these damaged mitochondria have a replicative advantage Decreased autophagocytosis of defective mitochondria Fig Scheme illustrating the mechanisms of lipofuscin...

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... is mainly associated with an increase in accessible thiol groups on the cell surface The results obtained in the present study clearly identify the implication of exofacial thiol groups in the ... the end of growth The latter thiol concentration was correlated to the reducing activity Indeed, a decrease in Eh7 was linked to an increase in exofacial thiol groups (Fig 5A, phase 1) and the Eh7 ... surface The addition of NEM or AMdiS to a reducing culture of L lactis rapidly increased the Eh7 and restored the initial Eh7 value (Fig 3) These data show the implication of thiol groups in the...