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INTERLEUKIN 6 RELEASE FROM t98g HUMAN GLIAL CELL LINE AS a PREDICTIVE MARKER FOR CHRONIC PAIN, AND THE CHARACTERIZATION OF SUBSTANCE(S) INVOLVED IN PAIN

INTERLEUKIN 6 RELEASE FROM t98g HUMAN GLIAL CELL LINE AS a PREDICTIVE MARKER FOR CHRONIC PAIN, AND THE CHARACTERIZATION OF SUBSTANCE(S) INVOLVED IN PAIN

INTERLEUKIN 6 RELEASE FROM t98g HUMAN GLIAL CELL LINE AS a PREDICTIVE MARKER FOR CHRONIC PAIN, AND THE CHARACTERIZATION OF SUBSTANCE(S) INVOLVED IN PAIN

... aspartate, arginine, asparagine, tyrosine and valine were also demonstrated to be elevated in the CSF of chronic pain patients as compared to that of acute pain patients On the other hand, GABA ... microglia are involved in the early development of chronic pain, while astrocytes function in sustaining the pain (Vallejo et al., 2010) An interesting finding was that nerve injury induces an increase ... Pain The International Association for the Study of Pain defines chronic pain as pain that persists for at least months Chronic pain is normally triggered by injury or disease, which damages the...
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Signal sequence analysis of expressed sequence tags from the nematode Nippostrongylus brasiliensis and the evolution of secreted proteins in parasites potx

Signal sequence analysis of expressed sequence tags from the nematode Nippostrongylus brasiliensis and the evolution of secreted proteins in parasites potx

... [53] The closest free-living taxa to the Strongylida are members of the Rhabditina, including C elegans, and both are grouped in Clade V of the Nematoda, on the basis of small subunit rRNA sequence ... existing entries), using a cutoff score of 80 in BLASTX (P < e-10) The number of ESTs bearing potential signal sequences was then calculated and the results are shown here (b) Effects of relaxing ... for parasite secreted proteins indeed acquired signal peptides, or have free-living lineages lost these motifs in the genes in question? Is more rapid diversification of secreted proteins a specific...
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Enterprise Adjustment and the Role of Bank Credit in Russia: Evidence from a 420 Firm''''s Qualitative Survey pdf

Enterprise Adjustment and the Role of Bank Credit in Russia: Evidence from a 420 Firm''''s Qualitative Survey pdf

... good enterprises, they appear unable to ration bad ones Enterprise Adjustment and the Role of Bank Credit in Russia: Evidence from a 420 Firm's Qualitative Survey ENTERPRISE ADJUSTMENT AND THE ROLE ... Enterprise Adjustment and the Role of Bank Credit in Russia: Evidence from a 420 Firm's Qualitative Survey In 1996, the credit has overall been decreasing for 53% of the sample, it has been increasing ... passive, and will appear as endogenous variable in section 10 Enterprise Adjustment and the Role of Bank Credit in Russia: Evidence from a 420 Firm's Qualitative Survey resources as an important...
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Báo cáo khoa học: Three-dimensional structure of a thermostable native cellobiohydrolase, CBH IB, and molecular characterization of the cel7 gene from the filamentous fungus, Talaromyces emersonii ppt

Báo cáo khoa học: Three-dimensional structure of a thermostable native cellobiohydrolase, CBH IB, and molecular characterization of the cel7 gene from the filamentous fungus, Talaromyces emersonii ppt

... was 0.7 and overall G-factor, a measure of the normality of the structure, was 0.0 Fig SDS/PAGE and activity analysis of recombinant cellobiohydrolase (A) SDS/ PAGE [10% (w/v) gel] analysis of ... (1985) Isolation and characterization of the endoglucanases of Talaromyces emersonii Biochem J 225, 365–374 29 McHale, A & Coughlan, M (1988) Purification of beta glucosidases from Talaromyces emersonii ... classification of cellulases Eur J Biochem 258, 200–206 68 Takashima, S., Iikura, H., Nakamura, A. , Hidaka, M., Masaki, H & Uozumi, T (1998) Isolation of the gene and characterization of the enzymatic...
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Báo cáo sinh học:

Báo cáo sinh học: "Glutamate carboxypeptidase activity in human skin biopsies as a pharmacodynamic marker for clinical studies" docx

... tissue samples easily assessable in human subjects Abbreviations GCP: glutamate carboxypeptidase; NAAG: N-acetyl-aspartyl-glutamate; NAA: N-acetyl-aspartate; CSF: cerebrospinal fluid; CNS: central ... rat and pig orthologs Prostate 2008, 68:171-182 doi:10.1186/1479-5876-9-27 Cite this article as: Rojas et al.: Glutamate carboxypeptidase activity in human skin biopsies as a pharmacodynamic marker ... Page of Chromatographic analysis was performed using a Waters ACQUITY UPLC (Milford, MA, USA) Separation of the analytes from potentially interfering material was achieved at ambient temperature...
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Báo cáo sinh học:

Báo cáo sinh học: " Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues" doc

... for retrieval of viral pathogens from infected leaf tissues and for the detection of viralderived transgene sequences in transgenic plants Results Use of FTA for sampling, retrieval and PCR-based ... Effective methods for sampling, storage and retrieval of viral pathogens from infected plant tissues allows not only identification of the viral pathogens but also detailed molecular study of their genomes, ... consisted of 30 cycles of 94°C for min., 58°C for and 72°C for MSV F &MSVR : PCR conditions consisted of 30 cycles of 94°C for min, 59°C for and 72°C for mins C1F &C2R: PCR conditions consisted of 94°C...
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báo cáo hóa học:

báo cáo hóa học:" Glutamate carboxypeptidase activity in human skin biopsies as a pharmacodynamic marker for clinical studies" doc

... tissue samples easily assessable in human subjects Abbreviations GCP: glutamate carboxypeptidase; NAAG: N-acetyl-aspartyl -glutamate; NAA: N-acetyl-aspartate; CSF: cerebrospinal fluid; CNS: central ... rat and pig orthologs Prostate 2008, 68:171-182 doi:10.1186/1479-5876-9-27 Cite this article as: Rojas et al.: Glutamate carboxypeptidase activity in human skin biopsies as a pharmacodynamic marker ... Page of Chromatographic analysis was performed using a Waters ACQUITY UPLC (Milford, MA, USA) Separation of the analytes from potentially interfering material was achieved at ambient temperature...
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báo cáo hóa học:

báo cáo hóa học:" Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues" doc

... for retrieval of viral pathogens from infected leaf tissues and for the detection of viralderived transgene sequences in transgenic plants Results Use of FTA for sampling, retrieval and PCR-based ... Effective methods for sampling, storage and retrieval of viral pathogens from infected plant tissues allows not only identification of the viral pathogens but also detailed molecular study of their genomes, ... consisted of 30 cycles of 94°C for min., 58°C for and 72°C for MSV F &MSVR : PCR conditions consisted of 30 cycles of 94°C for min, 59°C for and 72°C for mins C1F &C2R: PCR conditions consisted of 94°C...
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Báo cáo y học:

Báo cáo y học: " A predictive model for respiratory syncytial virus (RSV) hospitalisation of premature infants born at 33–35 weeks of gestational age, based on data from the Spanish FLIP study" pdf

... hospitalisation of 75% of at risk infants was calculated to be 11.7, assuming a 5% hospitalisation rate (consensus of European RSV Risk Factor Study Group based on a review of the available data [1,7,8]) ... collaborative network on infections in Canada study of predictors of hospitalization for respiratory syncytial virus infection for infants born at 33 through 35 completed weeks of gestation Pediatr ... within the base dataset Although the FLIP study [9] contained a great deal of information on risk factors and hospitalisation rates for children born between 33–35 weeks' GA, it was limited by being...
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Effects of high glucose concentrations on the expression of genes involved in proliferation and cell fate specification of mouse embryonic neural stem cells

Effects of high glucose concentrations on the expression of genes involved in proliferation and cell fate specification of mouse embryonic neural stem cells

... EFFECTS OF HIGH GLUCOSE CONCENTRATIONS ON THE EXPRESSION OF GENES INVOLVED IN PROLIFERATION AND CELL- FATE SPECIFICATION OF MOUSE EMBRYONIC NEURAL STEM CELLS FU JIANG (MD, MMed) A THESIS ... Illustration Schematic summary of the effects of high glucose on the expression of developmental control genes that are involved in proliferation, survival and differentiation of neural stem cells ... promote the differentiation of autonomic neurons and induce the expression of Ascl1 indicating that BMP2 and BMP4 control the specification of autonomic neurons through the induction of Ascl1 expression...
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Human embryonic stem cells as a cellular model for osteogenesis in implant testing and drug discovery

Human embryonic stem cells as a cellular model for osteogenesis in implant testing and drug discovery

... using human embryonic stem cells and derivatives as cellular model in implant testing for two main reasons One, human embryonic stem cells are the very original cells that our human body is developed ... OCT4 F: CGRGAAGCTGGAGGAGAAGGAGAAGCTG 55 oC R: AAGGGCCGCAGCTTACACATGTTC NANOG F: GGCAAACAACCCACTTCTGC 55 oC R: TGTTCCAGGCCTGATTGTTC β-ACTIN F: ACAGAGCCTCGCCTTTGCC 58 oC R: ACATGCCGGAGCCGTTGTC 2.1.5 ... creating iPSCs from human adult somatic cells by two independent teams led by Shinya Yamanaka and James Thomoson Yamanaka‘s group used the same retroviral system as they did for mouse fibroblasts...
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Báo cáo y học:

Báo cáo y học: " PKA and Epac cooperate to augment bradykinin-induced interleukin-8 release from human airway smooth muscle cells" docx

... 12 Augmentation of bradykinin-induced IL-8 release in hTERT -airway smooth muscle cells by Epac and PKA Augmentation of bradykinin-induced IL-8 release in hTERT -airway smooth muscle cells by Epac ... IL-8 release from hTERT -airway smooth muscle cells is regulated by both PKA and Epac In agreement with studies in human airway smooth muscle [22], the β2-agonist fenoterol and the distinct PKA/ Epac- related ... < 0.01, §§§P < 0.001 compared to basal condition PKA and Epac cooperate to activate Rap1 and to augment bradykinin-induced IL-8 release from human airway smooth muscle Studies on the molecular...
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Glial cell line drived neurotrophic factor (GDNF) family of ligands is a mitogenic agent in human glioblastoma and confers chemoresistance in a ligand specific fashion

Glial cell line drived neurotrophic factor (GDNF) family of ligands is a mitogenic agent in human glioblastoma and confers chemoresistance in a ligand specific fashion

... and Akt in both LN-229 and A1 72 human glioblastoma cell lines GDNF was however found to reduce the background activation of JNK and the A1 72 human glioblastoma cell line in a timedependent fashion ... potentiate adjuvant therapy It is likely that the chemoresistance properties are potentiated by autocrine and paracrine pathways and facilitated by mitogenic agents Local tissue invasion distinguishes ... treatment311 In the light of the evidence, we examined the modulation of MAPK and Akt signaling pathways in glioblastoma cell lines LN-229 and A1 72 human glioblastoma cell 12 lines were stimulated...
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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx

Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx

... TTCTCGAGATGGGAAAGTCTTCAGAGT GGT ACMSD real-time PCR: primer and probe ⁄ 3fw TGGCCAGATCTAAAAAAGAGGT 2fw ATCCCAGGAAACACCAGTAGA 10rev ATTGTTTTCTCTCAAGACCCAA TaqMan probe T1 ACACCACAGCAAGGGAGAAGCAAAG ... kit (Stratagene, La Jolla, CA, USA) Mutagenic primers were: 5¢-CGCTCGAGA TGAAAATTGACATCGCTAGTCATATTCTACC-3¢ and its complement for His6Ala; 5¢-GACATCCATAGTGCT ATTCTACCAAAAGAATGGCC-3¢ and its ... milk, pancreatic juice and intestine: inadequate for role in zinc absorption Am J Clin Nutr 35, 1–9 Evans GW & Johnson PE (1980) Characterization and quantitation of a zinc binding ligand in human...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx

Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx

... to bind lipid compounds within the different endolysosomal compartments [23] In this study, we examined whether iron loading of the liver epithelial cell line HepG2 in uenced the expression of ... stress, cell growth and lipid metabolism in the iron loaded HepG2 The results of the study revealed a new link between iron loading and lipid accumulation, leading to upregulation of CD1d molecules ... background staining (thin lines) Cells were then acquired in a FACScalibur and analyzed using CELLQUEST (A) Histograms show the expression of the molecules studied in HepG2 cells cultured in the absence...
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