Targeting polo like kinase 1 in glioma propagating cells

... of glioma 1. 4 Re-defining assay criteria for detecting GPCs 10 1. 5 PLK1 regulation and physiological role 11 1. 5 .1 PLK1 regulation 11 1. 5.2 Physiological role of PLK1 13 1. 5.3 PLK1 and tumors 15 ... 1. 9 34   3.3 PLK1 mRNA expression is elevated in glioma tumors PLK1 over-expression is common in several cancers of the breast 116 , ovaries 117 , prostate 118 and skin 119 In addition, PLK1 protein ... implicated in GPC survival, thus validating our screening method; PI3K/AKT, GSK3, CDK1, and TAK1 inhibitors 114 -11 5 Interestingly, a compound targeting PLK1 emerged, potentially identifying PLK1 as...

... strand, while the silencing activity of the siRNA was maintained [18] Polo-like kinase-1 (PLK-1) belongs to the family of serine/threonine kinases and regulates cell division in the mitotic phase ... the systemic siRNA delivery with atelocollagen existed intact for at least days in tumor tissues using a mouse model [62] Preclinical application of RNAi therapy against PLK-1 in a murine advanced ... NSCLC: non-small cell lung cancer; nt: nucleotide; PAZ: Piwi/ Argonaute/Zwille; PLK-1: Polo-like kinase-1; RISC: RNA-induced silencing complex; RNAi: RNA interference; siRNA: small interfering RNA;...

... (Polo- like kinase of X laevis); Snk (serum-inducible kinase) ; Fnk (fibroblastgrowth-factor-indiucible kinase) ; Prk (proliferation-related kinase) ; Sak (Snk akin kinase) xvi Table II: Polo- like kinase ... hours after transfection Caspase-3 activity assay was carried out (Fig 8) and intrigued to find that caspase-3 activation in si-PLK1 transfected Huh-7 was absent in the first 12 hours and 24 ... in this phase has been identified as a possible target of ataxia telangiectasia mutated (ATM) or ATM-related proteins (ATR), the transducers of the DNA damage signaling pathway (van Vugt et al.,...

... removal of cellular debris by phagocytes Transient appearance of low affinity anti-single-stranded or dsDNA antibodies, preferentially of IgM and < /b> IgG3 isotypes, will probably accompany any systemic ... secondary to cellular activation [116] Interestingly, medications that can cause drug-induced lupus (e.g hydralazine and < /b> procainamide) are capable of blocking methyltransferase activity [117], and < /b> ... autoreactive < /b> B cells < /b> and < /b> plasmacytoid < /b> dendritic < /b> cells < /b> with inhibitory DNA sequences Halpern and < /b> Pisetsky made the original observation that certain poly-G ODNs containing the nuclease-resistant...