Microfluidics and microarray based approaches to biological analysis 2
Microfluidics and microarray based approaches to biological analysis 2
... SiOH Si O Si O CH2 + (MeO)3 Si Si CH3 CONH2 OMe (CH2)3 O (CH2)3 CH2 O CH2 O Si O Si CONH2 CH2 OMe (CH2)3 CH3 TEMED, Persulfate CH3 CH Si SiOH O C CH2 CH3 CH2 CH CONH2 Figure 2. 14 Reaction scheme ... 150 20 0 -6 -6.05 -6.1 -6.15 -6 .2 -6 .25 -6.3 Figure 2. 12 Ln(µ) as function of the number of base pairs (N) 40 25 0 Chapter u (*10E4cm2V1s-1) 1.9 1.85 1.8 1.75 1.7 1.65 1.6 1.55 1.9 2. 1 2. 3 2. 5 2. 7 ... throughput PCR amplification in a silicon based array .21 2. 2 Results and Discussion 2. 2.1 Method Development Based on Conventional Capillary Electrophoresis 2. 2.1.1 Optimization of Separation Condition...
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Microfluidics and microarray based approaches to biological analysis 5
... anti-phosphoserine and antiphosphotyrosine antibodies was developed Due to its sensitivity, high specificity and the short incubation time needed to detect phosphorylation, this antibody -based approach ... activities, and potentially other enzymatic activities The intercalation of various PEGs between the slide surface and the immobilized peptides minimized non-specific binding of proteins to the glass ... methods, and may find wider uses in high-throughput kinase screenings The site-specific immobilization of proteins is even more critical since their active site needs to be accessible to the substrates...
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Microfluidics and microarray based approaches to biological analysis 4
... highlighted by its ability to withstand a variety of chemical conditions, which may make this new protein array compatible with most biological assays 4. 4 Materials and Methods 4. 4.1 Chemicals All chemical ... pH 7 .4, and spotted onto the avidin functionalized slides using an ESI SMA arrayer (Toronto, Canada) No incubation was necessary before the slide were further processed by washing with PBS and ... was washed with PBST as described earlier, and the specific binding between GST and glutathione was visualized with an ArrayWoRx microarray scanner 4. 4 .4 Treatment of Avidin Slides Under Harsh...
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Microfluidics and microarray based approaches to biological analysis 3
... a solution of 120 mM DIEA and 100 mM benzylmercaptan in DMF 94 Chapter 3. 3 .3 Array -Based Kinase Assay 3. 3 .3. 1 Spotting The N-terminally cysteine-containing peptides and N-terminally biotinylated ... washed, dried, scanned (Figure 3. 6) and the fluorescence intensity of the spots was measured (Figure 3. 7) 73 Chapter 3 mM mM 0 .3 mM 0.1 mM Figure 3. 6 Antibody -based fluorescence measurement of ... functionalized with (a) NHS PEG and (b) Amine PEG, and probed with FITC-labeled anti-phosphotyrosine 3. 2 .3 Rapid Microarray- Based Kinase Assay Rapid fluorescent antibody -based detection was combined...
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Microfluidics and microarray based approaches to biological analysis 1
... Kobayashi, M.; Horton, H.; Brown, E L Nat Biotechnol 19 96, 14 , 16 75 16 DeRisi, J L.; Iyer, V R.; Brown, P O Science, 19 97, 278, 680 17 Hacia, J Nat Genet 19 99, 21, 42 17 Chapter 18 Schena, M.; Shalon, ... target genes 1. 2.2 From Genomics to Proteomics 1. 2.2 .1 Limits of DNA Microarray- Based Strategies DNA microarray- based strategies allow for a detailed understanding of the regulation of biological ... Chapter 1. 1 .1 Capillary Electrophoresis and Microchip -Based Capillary Electrophoresis 1. 1 .1. 1 Capillary Electrophoresis The feasibility of performing free solution -based electrophoresis...
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Báo cáo khoa học: "Paragraph-, word-, and coherence-based approaches to sentence ranking: A comparison of algorithm and human performance" ppt
... paragraph as important, and the other sentences as not important We included this approach merely as a simple baseline 2.2 Word-based approaches Word-based approaches to summarization are based ... data Conclusion The goal of this paper was to evaluate the results of three different kinds of sentence ranking algorithms and one commercially available summarizer In order to evaluate the algorithms, ... shows average rank correlations (ρavg) of each algorithm and human sentence ranking for the 15 texts MarcuAvg refers to the version of Marcu (2000)’s algorithm where we calculated sentence rankings...
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An effective trajectory based algorithm for ball detection and tracking with application to the analysis of broadcast sports video
... are based on the location and trajectory of the ball For example, touching of the ball (a player coming into contact with the ball physically), kicking of the ball, and passing of the ball These ... AN EFFECTIVE TRAJECTORY- BASED ALGORITHM FOR BALL DETECTION AND TRACKING WITH APPLICATION TO THE ANALYSIS OF BROADCAST SPORTS VIDEO YU XINGUO (M.Eng, NTU) A THESIS SUBMITTED FOR THE DEGREE OF ... enhance the accuracy of ball detection and tracking in BSV The details of the methods and the results obtained are described further in this thesis Ball Detection and Tracking in Tennis: With the...
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Báo cáo y học molecular approaches to the analysis of deformed wing virus replication and pathogenesis in the honey bee, apis mellifera
... highly prevalent honey bee virus that causes wing deformity and mortality in honey bees worldwide, in both bees with wing deformity (symptomatic infection) and bees with normal wings (asympto- In ... of viruses in the honey bee, Apis mellifera J Invertebr Pathol 2006, 92:152-159 Chen Y, Pettis JS, Feldlaufer MF: Detection of multiple viruses in queens of the honey bee Apis mellifera L J Invertebr ... with the virus spreading to secondary sites from the digestive tract The presence of positive-strands, likely from food, and the absence of replication (negative-strands) of DWV RNA only in the...
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Asset based approaches to poverty reduction moser 2
... introduction to asset- based approaches to poverty reduction in a globalized context The aim is to show the added value of asset- based approaches, in terms of both better understanding poverty and ... Bank 32 Appendix 1: Workshop Program Brookings Institution / Ford Foundation Workshop Asset- based Approaches to Poverty Reduction in a Globalized Context 27 th – 28 th June 20 06 Washington DC ... Foundation Workshop on Asset- based approaches to poverty reduction in a globalized context, held in Washington DC on 27 –8 June 20 06 (see appendix for conference details).3 The asset accumulation...
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Báo cáo khoa học: The use of recombinant protein and RNA interference approaches to study the reproductive functions of a gonad-stimulating hormone from the shrimp Metapenaeus ensis ppt
... either: (a) dsRNA; or (b) small interference (si )RNA The longer dsRNA may generate a large population of siRNA (with 21–23 nucleotides), and the use of longer dsRNA may be advantageous over siRNA ... corresponding to the mature peptide of MeMIH-B was amplified by PCR using T7 promoter-linked primers (forward, 5¢-TAATACGACTCACTATAGGTACTATG TATCGCATGCCAAT-3¢; reverse, 5¢-TAATACGACTC ACTATAGGTACTTTAAAGTCCCGGGTTGA-3¢) ... culture tanks At 24, 48 and 72 h after injection, the hepatopancreas and ovary of the shrimp were dissected for total RNA preparation, and the hemolymph samples were collected for SDS ⁄ PAGE and western...
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Marrying Prevention and Resiliency - Balancing Approaches to an Uncertain Terrorist Threat docx
... Nuclear, and Biological Terrorist Attacks, Santa Monica, Calif.: RAND Corporation, MR-1731-SF, 2003 12 Marrying Prevention and Resiliency: Balancing Approaches to an Uncertain Terrorist Threat ... iv Marrying Prevention and Resiliency: Balancing Approaches to an Uncertain Terrorist Threat Wall: Understanding Terrorist Efforts to Overcome Defensive Technologies, Santa Monica, Calif.: RAND ... what the terrorist is trying to Marrying Prevention and Resiliency: Balancing Approaches to an Uncertain Terrorist Threat Methods for Dealing with Uncertainty: Lessons from Defense Planning Problems...
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Urban Health and Society: Interdisciplinary Approaches to Research and Practice - Part 35 doc
... director, Institute on Urban Health Research, Northeastern University Urban Health and Society: Interdisciplinary Approaches to Research and Practice provides students in public health, urban ... approach to racial and, 135 136; research on health and, 21, 34–37, 143; urban food environments and, 47 Hurricane Katrina: destruction and deaths from, 224–225; social-ecological study of health ... understand or reduce urban health risks, create safe urban environments, and deliver effective and sustainable health services and programs to urban populations.” —Stephen Lepore, professor and...
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Urban Health and Society: Interdisciplinary Approaches to Research and Practice - Part 1 doc
... Foreclosure The Research and Its Context Focus Group Analysis and the Emergence of Health as an Issue vii 93 94 95 96 96 97 10 1 11 0 11 1 11 7 11 9 11 9 12 7 12 8 13 1 13 8 14 4 14 8 16 1 16 2 16 4 16 6 17 0 viii Contents ... Katrina September 11 , 20 01, Terrorist Attacks on New York City Implications for Prevention and Intervention Summary 18 3 18 5 18 6 18 6 18 7 18 9 19 6 205 207 211 217 218 223 224 226 229 2 31 ... and Public Health 17 3 Neoliberalism, the Foreclosure Crisis, and Health Consequences 17 4 Conclusion 17 6 Summary 17 8 PART THREE INTERDISCIPLINARY APPROACHES TO INTERVENTIONS TO PROMOTE URBAN HEALTH...
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Urban Health and Society: Interdisciplinary Approaches to Research and Practice - Part 2 pps
... edited Urban Health and Society: Interdisciplinary Approaches to Research and Practice to prepare researchers and practitioners to be better equipped to meet the challenges of improving the health ... courses on interdisciplinary approaches to urban health for masters and doctoral students in public health and the social sciences, and collaborated on research projects aimed at understanding how ... teachers, researchers, and policy advocates motivated us to compile this book At City University of New York, we have worked together to develop interdisciplinary approaches to teaching and research, ...
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Urban Health and Society: Interdisciplinary Approaches to Research and Practice - Part 3 docx
... population lived in urban areas, and by 2 030 , three-quarters will live in cities.1, For health researchers and practitioners, understanding how the urban environment influences health and well-being will ... anthropology, psychology, urban planning, geography, and other disciplines to the concepts of interdisciplinary approaches to urban health research and practice Our goals are to familiarize readers ... concepts and principles that characterize interdisciplinary urban health research, to provide case studies of interdisciplinary health research within cities, and to prepare readers to work more...
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Từ khóa: Báo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018Báo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Định tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Thiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2chuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015Đổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namMÔN TRUYỀN THÔNG MARKETING TÍCH HỢP