Gene expression of thyroid hormone receptors TR alpha and TR beta during early development in tilapia, oreochromis mossambicus

... thyroid hormones on TRs was investigated (Chapter 5) Cloning of TR and TR CHAPTER CLONING AND SEQUENCING OF THYROID HORMONE RECEPTORS TR AND TR IN TILAPIA, OREOCHROMIS MOSSAMBICUS 2.1 INTRODUCTION ... TR isoforms, including TR 1, TR 2 and TR 3 from the TR gene and TR 1 and TR 2 from the TR gene (Lazar, 1993) The TR isoforms differ in their carboxyl-termini The TR 1 isoform binds to T3 and ... distribution, their expression during early development and the autoinduction of these receptors in the tilapia, O mossambicus In order to understand gene expression of TRs during development in O mossambicus, ...

Báo cáo khoa học: Identiﬁcation of alternative promoter usage for the matrix Gla protein gene Evidence for differential expression during early development in Xenopus laevis doc

... for the ﬁrst time, the presence of alternative promoter usage for the MGP gene and provides clear evidence for differential expression of this gene during the very early stages of embryonic development ... maternally inherited, and we provide evidence for the presence of alternative promoter usage in this gene during early X laevis development Results Identiﬁcation of a functional proximal promoter for ... evidence for maternal inheritance of the shorter MGP transcript and alternative promoter usage during early X laevis development is 1505 Alternative promoter usage for Xenopus MGP gene Fig Transcriptional...

Báo cáo khoa học: " Expression of pituitary adenylate cyclase activating polypeptide and its type I receptor mRNAs in human placenta" doc

... N, Fujii Y, Gonoi T, Miyazaki J, Seino S Cloning and functional characterization of a third pituitary adenylate cyclaseactivating polypeptide receptor subtype expressed in insulinsecreting cells ... not offer any information as to the distribution of PACAP and PAC1 receptor in human placenta at various gestations, during pregnancy In this study, we utilized in situ hybridization to determine ... Pituitary adenylate cyclase- activating polypeptide, interleukin-6 and glucocorticoids regulate the release of vascular endothelial growth factor in pituitary folliculostellate cells J Endocrinol...

Multi-tissue gene-expression analysis in a mouse model of thyroid hormone resistance docx

... of adenomatous polyposis coli (APC), somatostatin, carbonic anhydrase and tenascin C in the cerebellum (Figure 1a) and those of lysyl oxidase, carbonic anhydrase 4, enolase 3β and creatine kinase ... sensitive to thyroid hormone and the primary mode of T3 action is a direct influence on cardiac gene expression [20] Increased heart rate (tachycardia) and myocardial contractility are clinical features ... selection and data analysis All array data were deposited in the NCI-CIT microarray database [65] where Cy3 and Cy5 signals were median normalized and expression ratios (Cy5/Cy3) were calculated The...

Báo cáo khoa học: Light-induced gene expression of fructose 1,6-bisphosphate aldolase during heterotrophic growth in a cyanobacterium, Synechocystis sp. PCC 6803 ppt

... 5¢-GGAAGAAC CGTGCATTACC-3¢, and labeled with [a- 32P]dCTP using a Megaprime labeling kit (Amersham Pharmacia, Piscataway, NJ, USA) Hybridization signals were detected with a BAS2000 bio-imaging analyzer ... protein Intracellular l-glutamate was assayed by measurement of the concentration of l-glutamate with a l-glutamate determination kit (Yamasa Corp., Chiba, Japan) 2D-PAGE and in- gel digestion The materials ... translation initiation site of the fbaA gene was ampliﬁed with forward primer 5¢-GCTAAAGGAAGTATTTGCTAC-3¢ and reverse primer 5¢-CTGAGTTAACCAAGTCCAGG-3¢ A 134 bp DNA fragment, prom4, corresponding...

Báo cáo khoa học: Silencing the expression of mitochondrial acyl-CoA thioesterase I and acyl-CoA synthetase 4 inhibits hormone-induced steroidogenesis potx

... Effect of MTE -I overexpression on steroidogenesis To further explore the role of MTE -I in the regulation of steroidogenesis, we examined the effect of MTE -I 1807 Acyl-CoA thioesterase and synthetase ... these two proteins is the rate-limiting step in steroidogenesis [6] Fatty acyl-CoAs bind to the acyl-CoA- binding protein, which can bind and may thereby activate PBR This interaction favors the ... 22(R)-OH-cholesterol-sustained steroid synthesis is not affected by a reduction in MTE -I expression conﬁrms a previously expected role of MTE -I in the regulation of the rate-limiting step in steroidogenesis through the...

thyroid hormone receptors

... Introduction 1 Introduction to Thyroid Hormone Receptors Aria Baniahmad Biology of Thyroid Hormone Receptors Thyroid hormone receptors (TRs) play a major role in animal physiology ... the super family of nuclear hormone receptors (NHR) These receptors represent hormone- regulated transcription factors Members of NHR include receptors for lipophilic hormones, such as steroids, ... Thyroid Hormone Thyroid hormone, isolated by Kendall in 1915, is one of the first hormones identified in the early last century Its chemical structure has been known since 1925 Thyroid hormone...

báo cáo hóa học: " Temporal expression and cellular origin of CC chemokine receptors CCR1, CCR2 and CCR5 in the central nervous system: insight into mechanisms of MOG-induced EAE" pptx

... cells in defined lesional stages Quantification of CCR1, CCR2 and CCR5 mRNA expressing Quantification of CCR1, CCR2 and CCR5 mRNA expressing cells in defined lesional stages Mean numbers of CCR1+ ... expression of CCR1, CCR2 and CCR5 in the spinal cord (data not shown) Enhanced expression of CCR1, CCR2 and CCR5 mRNA was subsequently observed over cells within inflammatory aggregates during the early ... of CCR1, CCR2 and CCR5 mRNA in the rat spinal cord No expression of CCR1, CCR2 or CCR5 was detected within the spinal cord of healthy control rats (Fig 2A, 2D, 2G) or MOG-EAE rats in the pre-symptomatic...

Báo cáo hóa học: " The effects of hypertonic fluid administration on the gene expression of inflammatory mediators in circulating leucocytes in patients with septic shock: a preliminary study" pptx

... effects of hypertonic fluid administration on the gene expression of inflammatory mediators in circulating leucocytes in patients with septic shock: a preliminary study Annals of Intensive Care 2011 ... solution was added and the sample incubated, shaking at 37°C for 30 to digest any contaminating DNA A total of μl of Stop solution was added, heated, and shaken at 65°C for 10 minutes, with the samples ... (Table 2) Gene expression at baseline The expression at baseline of all measured mediators was comparable between the two groups (Figure 1) The genes Page of Table Baseline characteristics Variables...

Báo cáo y học: "Differential gene expression of bone anabolic factors and trabecular bone architectural changes in the proximal femoral shaft of primary hip osteoarthritis patients" pptx

... mechanisms involved in the increase or maintenance of bone mass in OA This study of the IT region of the proximal femur in primary hip OA and non-OA postmortem controls investigated changes in gene expression ... of combining gene expression analysis with histomorphometry allows the exploration of any relationships between gene expression and indices of bone architecture and bone remodelling Interestingly, ... increased OB biosynthetic activity [18] The lack of differential gene expression of these anabolic growth factors in our study is most likely due to the gene expression being contributed by the...

Fibroblast-like synovial cells from normal and inflamed knee joints differently affect the expression of pain-related receptors in sensory neurones: a co-culture study ppsx

... prepared from the knee joints of normal rats or from rats days (acute phase) or 20 to 28 days (chronic phase of inflammation) after induction of AIA The patella and the menisci of the joints ... normal knee joints or from acutely or chronically inflamed knee joints from AIA rats FLS cells are key players in the propagation of joint inflammation and joint destruction during rheumatoid arthritis ... FLS cells and co-cultures of DRG neurones and FLS cells (from normal and acutely or chronically inflamed joints) were analysed for the production of prostaglandin E2 (PGE2) and IL-6 The samples...

Báo cáo y học: "Passage and reversal effects on gene expression of bovine meniscal fibrochondrocytes" pdf

... protein gene expression profiles of meniscal fibrochondrocytes The x-axis refers to the passage number, and the y- axis to protein gene expression profiles of meniscal fibrochondrocytes the gene expression ... II gene expression profiles of meniscal fibrochondrocytes The x-axis refers to the passage number, and the y- axis to the gene expression fibrochondrocytes abundance (in the exponent notation ... Collagen I gene expression profiles of meniscal fibrochondrocytes The x-axis refers to the passage number, and the y- axis to the gene expression fibrochondrocytes abundance (in the exponent notation...

Báo cáo y học: " Differential expression of Toll-like receptors on human alveolar macrophages and autologous peripheral monocytes" pdf

... analyze the constitutive expression of each of the TLR genes in bronchoalveolar cells and monocytes, TLR gene expression in autologous monocytes was set as 1, and the TLR gene expression of the autologous ... TLR9 on human alveolar macrophages and monocytes Alveolar macrophages and monocytes from healthy donors were analyzed by flow cytometry using phycoerythrin (PE)-coupled mouse anti-TLR2, TLR4 and ... 0.9 Constitutive surface expression of TLR2, TLR4 and TLR9 on human monocytes and alveolar macrophages and monocyte-derived macrophages TLR levels were determined on monocytes (MN, n = 8), alveolar...

Báo cáo khoa học: "The role of thyroid hormone therapy in acutely ill cardiac patients" pot

... Seo H: A κ potential role of activated NF-κB in the pathogenesis of euthyroid sick syndrome J Clin Invest 2000, 106:393-402 De Groot LJ: Dangerous dogmas in medicine: the nonthyroidal illness syndrome ... seriously in a prospective randomized trial Competing interests The author(s) declare that they have no competing interests References 334 Wartofsky L, Burman KD: Alterations in thyroid function in ... roles of the iodothyronine selenodeiodinases Endocr Rev 2002, 23:38-89 Iltumur K, Olmez G, Ariturk Z, Taskesen T, Toprak N: Clinical investigation: thyroid function test abnormalities in cardiac...

Báo cáo y học: " Expression of parathyroid hormone-related protein during immortalization of human peripheral blood mononuclear cells by HTLV-1: Implications for transformation" pps

... human lymphocytes by HTLV-1 Using long-term liquid culture immortalization assays, we showed that PTHrP and PTH1R were markedly up-regulated during immortalization of T-lymphocytes PTHrP expression ... transforming factor in conjunction with other oncogenes The goal of this study was to investigate the expression of PTHrP, its receptor, and MIP-1α during the early stages of immortalization of human ... variety of other disorders [1,2] ATLL is an aggressive malignancy of CD4+ T cells that occurs in approximately 5% of infected individuals after a long latency period of 20–40 years The long latency...

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