Genetic engineering and surface modification of baculovirus derived vectors for improved gene delivery to the central nervous system
Genetic engineering and surface modification of baculovirus derived vectors for improved gene delivery to the central nervous system
... GENETIC ENGINEERING AND SURFACE MODIFICATION OF BACULOVIRUS DERIVED VECTORS FOR IMPROVED GENE DELIVERY TO THE CENTRAL NERVOUS SYSTEM YANG YI (B Eng.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR ... Chapter Three: Genetic Engineering of Baculovirus Vectors for Controlled Gene Delivery ………65 3.1 Introduction………………………………………………………………… 66 3.1.1 Genetic Engineering of Baculovirus Vectors for Neuron-targeted ... SUMMARY The studies presented in this thesis focused on development and modification of baculovirus- based vectors in order to achieve improved gene delivery to the Central Nervous System There...
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Development of hybrid promoters and viral vectors for improved gene delivery to the central nervous system
... overview of the progress made by using different types of promoters and vectors for gene delivery to the CNS 1.1 Current progress in gene therapy Gene therapy, which usually refers to somatic gene therapy, ... by the selection of promoters The gene delivery vector that carries and transfers the gene into the nuclei of target cell is another key Chapter Introduction issue of gene therapy The tropism of ... 2003) 1.4.2 Viral vectors Viral vectors are the most efficient vectors to transduce mammalian cells, and therefore are employed in most gene delivery experiments A variety of viral vectors, including...
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Design of viral vectors for improved gene delivery
... Packaging of amplicon viral vectors 3.3.10 Analysis of cell cycle-dependent transgene expression in pC8-36 amplicon viral vectors 3.3.11 Effect of transduction of viral vector on the cell cycle profile ... (Castro et al., 2003a) 1.3.2 Gene therapy of gliomas One of the formidable tasks of intracranial gene delivery is the difficulty in achieving gene delivery to > % of the tumor mass It would be ... LIST OF TABLES Table 1.1 Vectors and delivery systems for gene therapy 12 Table 5.1 Determination of transduction efficiency of amplicon viruses in vivo 160 Table 5.2 Comparison of percentage of...
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The study of the mediation of ohanin, a king cobra (ophiophagus hannah) toxin through the central nervous system
... Animalia kingdom, Chordata phylum, Vertebrata subphylum, the Reptilia class and Squamata order (Evans, 2003) Venomous Snakes There are many different and diverse species of animals across all phyla ... Cobras, coral snakes, kraits, mambas, sea snakes, sea kraits and Australian elapids Viperidae (viperids) True vipers and pit vipers, including rattlesnakes Table Snakes that known to cause dangerous ... Ophiophagus hannah – King Cobra The King Cobra (Ophiophagus hannah) is the world's longest venomous snake, with a length that can be as large as 5.6 m This species is found throughout South-Eastern...
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báo cáo hóa học: " Temporal expression and cellular origin of CC chemokine receptors CCR1, CCR2 and CCR5 in the central nervous system: insight into mechanisms of MOG-induced EAE" pptx
... cells in defined lesional stages Quantification of CCR1, CCR2 and CCR5 mRNA expressing Quantification of CCR1, CCR2 and CCR5 mRNA expressing cells in defined lesional stages Mean numbers of CCR1+ ... expression of CCR1, CCR2 and CCR5 in the spinal cord (data not shown) Enhanced expression of CCR1, CCR2 and CCR5 mRNA was subsequently observed over cells within inflammatory aggregates during the early ... of CCR1, CCR2 and CCR5 mRNA in the rat spinal cord No expression of CCR1, CCR2 or CCR5 was detected within the spinal cord of healthy control rats (Fig 2A, 2D, 2G) or MOG-EAE rats in the pre-symptomatic...
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Báo cáo khoa học: "Immunohistochemical study of constitutive neuronal and inducible nitric oxide synthase in the central nervous system of goat with natural listeriosis" potx
... counterstained with hematoxylin Magnification A and B, ×66 C and D, ×132 Immunohistochmical study of nitric oxide synthase in the central nervous system of goat Enhanced expression of nNOS and iNOS in ... is the first study of NOS expression in the goat brain with listeriosis The involvement of iNOS in bovine and caprine listeriosis has been reported previously, and the increased expression of iNOS ... microabscesses, and some astrocytes (identical in Fig Histology and immunostaining of caprine brains with natural listeriosis A: Brain of a goat with listeriosis showing meningoencephalitis H-E staining,...
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báo cáo khoa học: "Isolated angiitis of the central nervous system with tumor-like lesion, mimicking brain malignant glioma: a case report and review of the literature" pdf
... Primary angiitis of the central nervous system: serial MRI of brain and spinal cord Neuroradiology 2001, 43:599-607 14 Moore PM: Diagnosis and management of isolated angiitis of the central nervous ... Neuropsychopharmacol Biol Psychiatry 2005, 29:627-631 Carandang C, Grant A: Delirium and Isolated Angiitis of the Central Nervous System: a Case Report and Review CNS Spectrums 2008, 13:209-213 Page of ... treatment protocols used in these cases Fountain et al [23] reported a case with IACNS controlled by cyclophosphamide alone, while Carandang C and Grant A reported a female patient with IACNS...
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Báo cáo sinh học: "The long term effects of chemotherapy on the central nervous system" docx
... vulnerable, population of patients Chemotherapy- induced cognitive decline in the absence of radiation The best data on the cognitive effects of chemotherapy alone have come from studies of children with ... high doses of chemotherapy is clearly demonstrated by Noble and colleagues [1], yet these proposed studies not take into consideration the possible effects of high-dose chemotherapy on either CNS ... are planned that further reduce the dose of craniospinal radiation to 1,800 cGy, virtually no attention has been paid to the possible contribution of the chemotherapy to the cognitive decline...
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Báo cáo sinh học: "Systemic 5-fluorouracil treatment causes a syndrome of delayed myelin destruction in the central nervous system" doc
... sections of CC displayed scattered foci of demyelinated axons, including partial or complete loss of myelin sheaths and increases in inter-laminar splitting of the myelin sheaths Analysis of transverse ... partially destructed myelin sheaths; single arrows indicate inter-laminar splitting of the myelin sheaths; and double arrows indicate myelin debris (b) Two axons with damaged myelin sheaths (asterisks), ... inflammation in a subset of treated animals The occurrence of delayed damage to the CNS following irradiation has been a subject of interest for many years, and both vascular damage and delayed inflammatory...
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báo cáo khoa học: "In vivo observation of gold nanoparticles in the central nervous system of Blaberus discoidalis" potx
... by the nAuNPs The XPS was used to verify the presence of nAuNPs embedded in the brain tissue The confocal microscope and TEM were used to gain insights into the interaction of nAuNPs and the insect’s ... analysis The hyperspectral imaging system from CitoViva was used to identify the location of the nAuNPs particles in the tested roach This imaging system identified the presence of gold in the tissues ... biological system, i.e the material surrounding the nanoparticles are proteins present in the nervous system of the cockroach Discussion The results of characterization have repeatedly proven that the...
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Báo cáo sinh học: " Improved gene delivery to human saphenous vein cells and tissue using a peptide-modified adenoviral vector Lorraine M Work1, Paul N Reynolds2 and Andrew H " pptx
... intact human saphenous vein ex vivo, the ultimate clinical target http://www.gvt-journal.com/content/2/1/14 HSVEC were obtained by enzymatic collagenase digestion of human saphenous vein and maintained ... with (A) HSVEC and (B) HSVSMC for different times and gene expression quantified and normalised to protein Representative fluorescent images are shown (C) Intact human saphenous vein was incubated ... and vein transduction experiments PNR produced the viruses and AHB supervised all work as principle investigator The authors thank Nicola Britton and Margaret Cunningham for technical assistance...
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The role of hydrogen sulfide in the central nervous system implications in the treatment of alzheimers disease
... THE ROLE OF HYDROGEN SULFIDE IN THE CENTRAL NERVOUS SYSTEM: IMPLICATIONS IN TREATMENT OF ALZHEIMER’S DISEASE BHUSHAN VIJAY NAGPURE (M.B.B.S., Maharashtra University of Health Sciences, India) ... lot of efforts have been put into the development of disease- modifying drug therapy Amyloidogenesis is one of the main culprits of AD pathology The effect of NaHS, a rapid exogenous hydrogen sulfide ... like tau is involved in microtubule assembly It serves as a binding for the tubulin Mutations in α-synuclein lead to loss of this binding ability, resulting in tubulin and α-synuclein aggregation...
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A study of the recombination activating gene 1 in the zebrafish nervous system
... PTU 1- phenyl-2-thiourea RACE Rapid amplification of cDNA End Rag1 Recombination activating gene Rag2 Recombination activating gene RAS Rat Sarcoma RGC Retina gaglion cell RING Really interesting ... about the neuronal function of Rag1 17 1. 3 Advantages of using zebrafish 18 1. 3 .1 Zebrafish as a model for developmental and genetic research in vertebrates 18 1. 3.2 Advantages of zebrafish in ... 2.6 .1 RAG1 and RAG2 antibodies Rabbit polyclonal antibodies against the C-terminus of zebrafish RAG1 (amino acids 10 42 ~10 57), and the C-terminus of zebrafish RAG2 (amino acids 517 ~530) were generated...
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INVESTIGATION OF MEMBRANE PROPERTIES IN THE CENTRAL NERVOUS SYSTEM OF DROSOPHILA MELANOGASTER STUDIED BY FLUORESCENCE CORRELATION SPECTROSCOPY
... Summary The objective of this study is to apply biophysical fluorescence technique, i.e fluorescence correlation spectroscopy (FCS), in situ in the central nervous system of fruit fly (Drosophila melanogaster) ... neuronal membrane fluidity was being studied in situ in the central nervous system of Drosophila melanogaster embryos by FCS Our approach promises to shed light on the biophysical features of cellular ... this is the first time confocal FCS is being used to study membrane fluidity in situ in the central nervous system of Drosophila melanogaster embryos A study by Sergent et al indicated that increased...
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Báo cáo hóa học: "Surface Morphological and Nanomechanical Properties of PLD-Derived ZnO Thin Films" docx
... surface roughness and evaluated mechanical properties of ZnO thin films and bulk materials (S.-R Jian* et al.) ZnO fiilm/sapphire ZnO film/c-sapphire ZnO films/(0001) 6H-SiC ZnO films ZnO (bulk) 4.1 ... properties of thin films/substrates systems Experimental Details The Growth Conditions of ZnO Thin Films ZnO thin films were grown on (0001) (c-oriented), ð1120Þ (a-oriented) sapphire and (0001) ... to the ZnO thin films, in merit of both basic research and technological applications In addition, nanoscratch technique can be used to characterize the nanotribological properties of ZnO thin films...
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