... DNA binding domain and VP16 activation domain Various combinations of the EcR ligand binding domain, VP16 activation domain and GAL4 DNA binding domain were constructed (Fig 1A) and tested in NIH ... probably in uence the behavior of fusion protein upon binding to ligand Apparently, having activation domain (VP16) and DNA binding domain (GAL4), in that order, on the NH2-terminal end of EcR ... formats Interestingly, this is the arrangement in natural EcRs [activation domain A ⁄ B, DNA binding domain (C) and ligand binding (E) domain] The difference in induction levels between cell lines...