Isolation and characterization of allergens from curvularia lunata 3
Isolation and characterization of allergens from curvularia lunata 3
... intensity of 20 3. 3 RESULTS AND DISCUSSION 3. 3.1 Cloning and Expression of the isolated putative C lunata allergens 3. 3.1.1 RACE amplification of the truncated putative allergen sequences Out of the ... 43. 4 28.8 25.6 33 .1 18.8 66.1 58.5 56.8 50.8 52.9 70.6 82.4 70.6 Cur l 13 C lunata A.fumigatus P.citrinum 14.5 32 .9 55 .3 40.8 25.0 35 .6 55.6 53. 1 59 .3 21.2 55.1 34 .7 70.6 35 .3 17.6 23. 5 128 Chapter ... Chapter 3. 1 INTRODUCTION In order to further understand and establish the allergenicity of the various putative allergens of Curvularia lunata, immunological characterization of the putative allergens...
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Isolation and characterization of allergens from curvularia lunata 1
... Mala s 18 AJ 011 955 Mala s 17 AJ 011 956 Mala s AJ 011 957 Mala s 19 AJ 011 958 Mala s 37 AJ 011 959 Mala s 10 heat shock prot 70 86 AJ428052 Mala s 11 Mn superoxide dismut 23 AJ5484 21 Mala s 12 glucose-methanol-choline ... view of these, identification of various allergenic components of Curvularia will contribute to a better understanding of the repertoire of Curvularia allergens, and of the allergies caused by Curvularia ... AJ223 315 X85092 Chapter Asp f 11 peptidyl-prolyl isomeras 24 Asp f 12 heat shock prot P90 90 Asp f 13 alkaline serine protease 34 Asp f 15 16 AJ002026 Asp f 16 43 g3643 813 Asp f 17 AJ224865 Asp f 18 ...
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Isolation and characterization of allergens from curvularia lunata 2
... 16 83 8 8 Curvularia pallescens 50 42 75 75 25 25 42 33 Curvularia fallax 42 33 42 75 33 25 25 25 Curvularia brachyspora 58 75 42 75 83 33 42 58 58 58 Curvularia lunata 75 42 58 92 25 33 58 Cladosporium ... SLVWEGSVLDSEEGIR 52. 129 ~10 8.84 199 VIGEVTLDQVYGGAR SLVWEGSVLDSEEGIR 52. 129 ~10 8.84 76 27 .844 ~10 6. 92 √ 74 108.534 ~15 6. 12 √ 64 48 .21 4 ~45 6 .24 91 Chapter 21 7 21 8 22 6 22 8 22 9 23 0 Protein recA ... contigs 021 058 119 171 21 9 28 1 350 3 92 449 506 5 92 655 697 756 821 891 % Redundancy 12. 00 19.00 28 .66 32. 50 33.80 35.83 38. 42 38 .25 38.77 39 .20 41.50 42. 00 41.38 41.85 42. 47 43.13 Figure 2. 3: Percentage...
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Isolation and characterization of allergens from curvularia lunata 4
... (%) of the allergens tested over various populations Allergen ClSOD AfSOD ScSOD HsSOD Populations tested Singaporean Indian Italian 44 .7 23.5 44 .4 18 .4 47.1 1.3 57.9 52.9 45 .0 32.9 35.3 27.5 144 ... other SODs from human and fungi suggesting that the levels of SODs in the indoor 149 Figure 4. 5: 3D models of various MnSODs Monomeric and tetrameric (top view) subunit of the SOD is taken from Sabine ... (%) of the allergens tested over various populations Allergen ClHsp AfHsp ScHsp HsHsp Populations tested Singaporean Colombian 28.9 44 .1 61.8 54. 2 27.6 42 .4 56.6 50.8 175 Chapter Figure 4. 24: ...
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Isolation and characterization of allergens from curvularia lunata 5
... Chapter 5. 1 DISCUSSION AND CONCLUSION Not many allergenic fungi are studied well and very few of them have been characterized for the allergens in detail Characterization of the fungus and its ... and critical residues for allergenicity, levels in the environment, function, etc Here, we report various allergens from Curvularia lunata For the rapid isolation of the allergenic proteins from ... of the allergens since it’s the three dimensional structure of the allergen which determines the interaction of the allergen to the IgE antibodies From the isolated C .lunata allergens, some of...
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Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx
... Purification of four pulchellin isoforms from A pulchellus seeds Using a combination of affinity, ion exchange and chromatofocusing chromatography, four pulchellin isoforms were isolated from A pulchellus ... II, III and IV and refers to each pulchellin Characterization of four pulchellin isoforms isoform (P I, P II, P II and P IV) The A-chain of P II was formerly cloned and named recombinant pulchellin ... III and P IV isoforms from the eluate P III ⁄ P IV (Fig 1C) Isoelectric focusing gave pI of 5.8, 5.7, 5.5 and 5 .2 for the four isoforms respectively Secondary structure of the pulchellin isoforms...
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Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx
... aminoacids at the N-terminus of PHI (sequence: SKVYLALQDND) were compared with the sequences of the so called ‘intermediate components’ from two other PHs The N-terminal sequence of PHI from A radioresistens ... one PHR and one PHO (abc) protomer The hypothesis of a direct PHI phenol interaction is quite unlikely, because of the fact that the addition of phenol does not alter the emission spectrum of PHI ... oxygenase [28–30], and, in the case of Pseudomonas CF600 phenol hydroxylase, via a direct interaction with the substrate itself [9], in the case of A radioresistens S13 phenol hydroxylase, PHI...
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Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt
... describes the relationship between the heme core description and the spectroscopic and redox properties of each identified heme from the NrfHA complex Conclusions D desulfuricans ATCC 27774 ccNiR ... this communication, we report for the first time the isolation and biochemical characterization of D desulfuricans ATCC 27774 ccNiR subunits The stoichiometry between NrfH and NrfA is discussed The ... (2003) Cytochrome c nitrite reductase ˜ from Desulfovibrio desulfuricans ATCC 27774 The relevance of the two calcium sites in the structure of the catalytic subunit (NrfA) J Biol Chem 278, 17455–17465...
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Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc
... highest identity with the BAS1 protein of Brassica, spinach, barley and A thaliana, PR1 of Phaseolus and MHF of A thaliana These proteins belong to the 2Cys-Prx subfamily All plant 2Cys-Prx proteins, ... very weak when Trx m from Chlamydomonas was used (data not shown), probably because of the weaker af®nity of Arabidopsis NTR for Trx m [21] Therefore, the ability of Trx m from Chlamydomonas ... protein and with BSA (400 lgámL)1) The reactions were stopped by adding 3.3 mM EDTA and analyzed on an agarose gel Thioredoxin-dependent peroxidase activity of Ch-Prx1 Peroxidase activity assays were...
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Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx
... Nagasawa T, Ohkishi H, Kawakami B, Yamano H, Hosono H, Tani Y & Yamada H (1982) 3-chloro-d-alanine chloride-lyase (deaminating) of Pseudomonas putida CR 1–1 Purification and characterization of ... molecular mass of 41.7 kDa and a pI of 6.34 The YedO protein from E coli and the d-CDes from Arabidopsis showed an overall identity of 36% and a similarity of 50% The blastp program in its default ... oxidases, transaminases, and racemases (epimerases) For example, in pea seedlings the occurrence of d-amino acid aminotransferase was demonstrated [36] For a number of other amino acids racemases...
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Báo cáo khoa học: " Isolation and characterization of Streptococcus sp.from diseased flounder (Paralichthys olivaceus )in Jeju Island" ppt
... eht fo esahp suoeuqa reppu eht no detcelloc saw AND lairetcaB nim rof 000,6 ta degufirtnec dna nim rof sllec lairetcab gniliob yb detcartxe neht saw AND lairetcab ;retaw dellitsid elbuod dezilirets ... 817( rof evitisop ,9~4 senal ;)pb 001,1( rof evitisop ,3 dna senal ;noitcefni laccocotperts rof evitagen ,1 enal ;lortnoc evitagen ,N enal ;)pb 003 ,M4040 ( lortnoc evitisop ,P senal ;reddal AND ... enilav ,esapil rof snoitcaer evitagen dah setalosi llA nispyrtomyhc -α dna esatahpsohp enilakla rof snoitcaer evitisop dah fo setalosi 81 dna esadinimasoculg-β-lyteca -N dna esaretse rof snoitcaer...
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Isolation and characterization of highly pathogenic avian influenza virus subtype H5N1 from donkeys docx
... Cite this article as: Abdel-Moneim et al., Isolation and characterization of highly pathogenic avian influenza virus subtype H5N1 from donkeys Journal of Biomedical Science 2010, 17:25 ... Fatal avian influenza A H5N1 in a dog Emerg Infect Dis 2006, 12:1744-1747 11 Li HY, Yu K, Yang H, Xin X, Chen J, Zhao P, Bi Y: Isolation and characterization of H5N1 and H9N2 influenza viruses from ... Experimental infection of cattle with highly pathogenic avian influenza virus (H5N1) Emerg Infect Dis 2008, 14:1132-1134 17 Cardona CJ, Xing Z, Sandrock CE, Davis CE: Avian influenza in birds and mammals...
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báo cáo khoa học: " Involvement of S-adenosylmethionine-dependent halide/thiol methyltransferase (HTMT) in methyl halide emissions from agricultural plants: isolation and characterization of an HTMT-coding gene from Raphanus sativus (daikon radish)" ppt
... Figure analysis of methyl halides, methanethiol, and DMS from R sativus GC-MS GC-MS analysis of methyl halides, methanethiol, and DMS from R sativus (a) GC-MS spectrum of methyl halide standards ... the emission profiles of methyl halides from some plants and the characterization of the enzymatic properties of recombinant R sativus HTMT (RsHTMT) Results and discussion Wuosmaa and Hager [14] ... substrate for HTMT in A thaliana Cloning and sequence analysis of an HTMT coding gene from R sativus To investigate the properties of HTMT in R sativus, a full length HTMT-encoding gene (Rshtmt) was...
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Báo cáo khoa học: "Isolation and characterization of a genotype 4 Hepatitis E virus strain from an infant in China" ppt
... Bezanilla JC, Toth TE, Meng XJ: Identification of genotype hepatitis E virus (HEV) in serum and faecal samples from pigs in Thailand and Mexico, where genotype and HEV strains are prevalent in ... genotype HEV strains with complete genome available in GenBank (including both swine and human original isolates) were used as references in the analysis; their GenBank accession numbers and source ... aimed to determined the whether this genotype HEV strain is prevalent in Shanghai swine or not HEV paly an important role in acute sporadic and epidemic viral hepatitis in Asia and Africa Although...
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Báo cáo y học: "Isolation and characterization of microparticles in sputum from cystic fibrosis patients" pot
... analysis of inflammatory cells infiltrating the cystic fibrosis airway mucosa Clin Exp Immunol 2001, 124(1):69-76 35 Livraghi A, Randell SH: Cystic fibrosis and other respiratory diseases of impaired ... largely sophisticated and quantifiable, and the search for novel biomarkers of CF airways disease in this biofluid is under way [16,47] The finding of MPs in sputum raises some intriguing questions ... endothelial cells, granulocytes, monocytes, lymphocytes) following chemical (cytokines, thrombin and endotoxin), physical (shear stress and hypoxia) [17] and apoptotic [18] stimuli One of the first described...
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