Protein separation in ion exchange membrane partitioned free flow isoelectric focusing (IEM FFIEF) system
Protein separation in ion exchange membrane partitioned free flow isoelectric focusing (IEM FFIEF) system
... INVESTIGATION OF MASS TRANSFER IN THE ION- EXCHANGE MEMBRANE PARTITIONED FREE- FLOW ISOELECTRIC FOCUSING SYSTEM (IEM- FFIEF) FOR PROTEIN SEPARATION …………………86 4.1 Introduction…………………………………………………………………………… ... Summary Ion Exchange Membrane Partitioned Free- Flow Isoelectric Focusing (IEM- FFIEF) is an emerging separation process combining both membrane technologies and electrophoresis technologies in a ... PROTEIN SEPARATION BY ION EXCHANGE MEMBRANE PARTITIONED FREE- FLOWISOELECTRIC FOCUSING SYSTEM …………………………………………………………………56 3.1 Introduction ………………………………………………………………………… 56 3.2 Working principle...
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Tài liệu Báo cáo khoa học: Mouse recombinant protein C variants with enhanced membrane affinity and hyper-anticoagulant activity in mouse plasma pptx
... proceeding clinical trials [27] lead us to develop an APC molecule with enhanced anticoagulant activity with the purpose of investigating the effect of APCs with increased anticoagulant activity ... each lane and visualized by silver staining Protein C variants and molecular weight markers (MWM) ran in each lane are indicated The location of heavy chain (HC), light chain (LC) and thrombin ... further improvement in membrane binding of the variants is shown in terms of membrane bind- Anticoagulant mouse protein C variants ing occupancy at the saturating protein concentration, a parameter...
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Tài liệu Báo cáo khoa học: The role of the SEA (sea urchin sperm protein, enterokinase and agrin) module in cleavage of membrane-tethered mucins pdf
... further investigate the role of the SEA domain in cleavage of membrane-tethered mucins we carried out site-directed mutagenesis of the FRPG ⁄ SVVV site and evaluated hybrid mucins in which the SEA ... previously The fragments were then cloned into the SpeI ⁄ XhoI sites of the polylinker in MUC1FDTR The remaining portions of the polylinker introduced leucine and valine residues N-terminal to the MUC3 ... SEA modules and mucin cleavage T Palmai-Pallag et al Table Conservation of the G ⁄ S cleavage site sequence in MUC1 from several species, other membrane-tethered mucins and SEA domain containing...
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Báo cáo khoa học: Protein transport in organelles: Protein transport into and across the thylakoid membrane pptx
... vivo but missing from in vitro experiments Transport into the thylakoid membrane Nuclear encoded proteins destined to be inserted into the thylakoid membrane are transported by either an assisted, ... transported protein is released from the translocase into the lipid bilayer, where the signal peptide is removed by the TPP and the mature protein is released into the lumen After protein transport, ... 5876–5883 Cline K, Ettinger WF & Theg SM (1992) Proteinspecific energy requirements for protein transport across or into thylakoid membranes Two lumenal proteins are transported in the absence...
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Báo cáo khoa học: Fluorescence studies of the replication initiator protein RepA in complex with operator and iteron sequences and free in solution pdf
... Fluorescence studies of RepA R E M Diederix et al Interestingly, in the latter case, the protein binds as a monomer [26] Free in solution, the protein is essentially dimeric, ... DNA-binding domains, and rearrangement of the relative orientation of the two domains [7,9] The conformational change upon iteron binding may expose a recognition site for proteinprotein interaction, ... latter protein, the crystal structures of both the monomeriteron and dimeroperator complexes are available, indicating secondary structural changes in the linker connecting the dimerization and...
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Báo cáo khoa học: Identification of tyrosine-phosphorylation sites in the nuclear membrane protein emerin pot
... for emerin Emerin is the product of a gene linked to EDMD [19,21] An integral membrane protein specifically located at the inner nuclear membrane, emerin, like other INM proteins, binds to lamins ... mislocalization of emerin that is caused by the loss of emerin binding to lamin A (X- linked form) or by the loss of lamin A (autosomal dominant form) [22,23] Several emerin binding partners have ... improving the search for phosphopeptides containing phosphotyrosine, we identified one of the well-defined integral INM proteins Emerin is a type-II integral membrane protein of 34 kDa, which is inserted...
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Báo cáo khoa học: Separation and characterization of caveolae subclasses in the plasma membrane of primary adipocytes; segregation of specific proteins and functions docx
... in the HD -caveolae and LD -caveolae; (c) the VHD -caveolae contained almost a third of the plasma membrane caveolin, and the majority of cellular caveolin is found in the plasma membrane of adipocytes; ... referring to all of them as caveolae, was demonstrated by their content of both caveolin-1 and caveolin-2 The coexistence of caveolin-1 and caveolin-2 in all three caveolae subclasses is in line ... lower in the LD -caveolae than in the closed VHD -caveolae, demonstrating that factors other than caveolin control the concentration of cholesterol in the plasma membrane and its domains Others...
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Báo cáo khoa học: Ion channel activity of brain abundant protein BASP1 in planar lipid bilayers pptx
... currents across planar lipid bilayers Results BASP1 exhibits single channel- like activity on planar lipid bilayers The activity of BASP1 and GAP-43 proteins in negatively-charged lipid bilayers under ... transformation by brain acid-soluble protein (BASP1) Proc Natl Acad Sci USA 106, 5604–5609 Ion channel activity of BASP1 35 Zakharov VV & Mosevitsky MI (2010) Oligomeric structure of brain abundant proteins ... within 180 (starting from the protein addition) The latency time before the appearance of ion channel activity was highly variable (in the range 30–120 min) and decreased with increasing protein...
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Báo cáo y học: "Tumour necrosis factor activates the mitogen-activated protein kinases p38α and ERK in the synovial membrane in vivo" doc
... vated protein kinase (MAPK)α, (b,e,h) extracellular signal-regulated kinase (ERK) , and (c,f,i) c-Jun amino-terminal kinase (JNK) were stained in both the synovial pannus (panels a–c) and the synovial ... wild-type mice (brown staining, black arrows) In contrast, JNK was activated far less frequently and only in a few cells within synovial pannus as well as the synovial lining in hTNFtg mice In wild-type ... indicating that the intracellular effects of TNF can be inhibited This suggests that anti-TNF therapy reduces key signalling pathways in the synovial membrane, such as the MAPKs, and thereby reduces...
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Regulating Lipid Organization and Investigating Membrane Protein Properties in Physisorbed Polymer-tethered Membranes
... Research Integrity and Copyright Disclaimer Title of Thesis/Dissertation: Regulating Lipid Organization and Investigating Membrane Protein Properties in Physisorbed Polymer-tethered Membranes ... 2011 Regulating Lipid Organization and Investigating Membrane Protein Properties in Physisorbed Polymertethered Membranes Major Professor: Christoph A Naumann Cell membranes have remarkable properties ... http://www.purdue.edu/policies/pages/teach_res_outreach/c_22.html REGULATING LIPID ORGANIZATION AND INVESTIGATING MEMBRANE PROTEIN PROPERTIES IN PHYSISORBED POLYMER-TETHERED MEMBRANES A Dissertation Submitted to the Faculty of Purdue University by Amanda...
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Molecular simulations of transport and separation in protein crystals
... found and modeled 55-59 Salts Refs Separation of mixtures in protein crystals has also been investigated including racemic separation. 1,5,64 In chiral and affinity separation, for instance, proteins ... in protein crystals using MD simulations, and subsequently provide molecular insights and guidelines for the development of high-performance protein crystals in separation technology First, protein ... the transport and separation in protein crystals 1.3.2 Simulation Studies Most simulation studies on proteins are focused on the conformational change (folding and unfolding) of a single protein...
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Mass transport enhancement in a proton exchange membrane fuel cell
... water and proton transport in catalyst layer and membrane, as well as electrical current transport in the bipolar plate The generalized Stefan-Maxwell equation is used to model the water and proton ... channels (parallel, serpentine, inter-digitated and etc) are machined in graphite plates to feed the reactant gases to the GDL Optimum flow channel area can be determined, as in some cases a larger ... results available from Temasek Poly, Singapore Carry out detailed parametric study in order to identify parameters that are critical for fuel cell operation For mass transport augmentation in forced...
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A Sneaky Backdoor In to Google FAST With Free Press Releases!
... originally wanted to make the main keyword “Soap Making” instead of “Soap Making Secrets” because soap making had over 22,000 searches the previous month… But I left it alone and wanted to see ... making, but also all the insider tricks, tips and techniques that the experts use to make advanced, hand-crafted soaps Based on years of research Dave doesn't leave the reader hanging "Learning ... free press release at PR-WEB you have a “Headline Section” a “Summary Section” and a “Body Section” The headline: Breakthrough Soap Making Secrets The Summary: Dave Cushion says: "These are Soap...
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Tài liệu Building Solutions in Microsoft® Exchange 2000 with the Web Storage System Classroom Setup Guide pptx
... Microsoft Excel 2000 ! Microsoft FrontPage® 2000 ! Microsoft Outlook® 2000 ! Workflow Designer for Exchange 2000 ! Web Storage System Tools (Web Storage System Explorer) ! Web Storage System SDK ! ... click OK Classroom Setup Guide 13 ! Configure the non-MAPI folder in Exchange System Manager Click Start, point to Programs, point to Microsoft Exchange, and then click System Manager In System ... WebLabs in the Directory name box and in the Title box, and then click Next In the Access Control dialog box, select Use the same administrator as the parent web, and then click Next Click Finish In...
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Tài liệu Module 7: SMTP in Microsoft Exchange 2000 pptx
... ONLY 26 Module 7: SMTP in Microsoft Exchange 2000 Configuring Global SMTP Parameters Topic Objective To describe global SMTP settings Maximum message size 2000 KB Lead -in Incoming Incoming There ... ONLY Module 7: SMTP in Microsoft Exchange 2000 45 Review Topic Objective To reinforce module objectives by reviewing key points ! Introduction to SMTP Lead -in ! Configuring Multiple SMTP Domain ... for Module 2, “Installing Microsoft Exchange 2000, ” in course 1572A, Implementing and Managing Microsoft Exchange 2000 ! Install Exchange 2000 at D:\Program Files\Exchsrvr on each server into...
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