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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 6

Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 2

Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 2

... (m, 22 H), 0. 92 (t, 3H); 13C NMR (300 MHz, C6D6) δ 154.8, 94.6, 85.6, 80 .2, 79.7, 79 .2, 65.0, 64 .2, 63.8, 63 .2, 62. 8, 61.9, 32. 0, 29 .8, 29 .6, 29 .4, 29 .2, 28 .9, 28 .7, 28 .0, 25 .8, 25 .4, 23 .2, 22 .7, ... 64.44% 72. 98% 63.88% Structures of six obtained compounds (analogues of sphingosine) 2. 2 .2 Compounds Inhibitory Function on SPHK Activity in vitro 2. 2 .2. 1 Over-expression of SPHK1 and SPHK2 2. 2 .2. 1.1 ... specific inhibitors for SPHK, and even isotype-specific (SPHK1 vs SPHK2) With the newly developed inhibitors, a better understanding of the role of SPHK in the process of stem cells differentiation...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 3

Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 3

... 3. 1 .3 Staining 3. 1 .3. 1 Alizarin Red S Staining Alizarin Red S staining is widely used to evaluate the calcium-rich deposits generated by cells In this study, the staining was used to detect the ... difference compared with the induced cells without inhibitors Interestingly and similarly with the hFOB test, 1μM of DMS and 5μM of CP6 inhibited the differentiation of the cells-induced for 28 days ... staining in each sample was quantified by dissolving the staining with 100% of isopropanol and detecting the absorbance at 500nm 3. 1 .3. 3 Fluorescence Immunostaining Fluorescence immunostaining...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 4

Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 4

... penetrating the cells and act on, as yet unknown, intracellular targets capable of triggering GPCR-independent calcium signals 4. 2.3 .4 MAPK (P42 /44 ) Activation and IκB Phosphorylation MAPK (P42 /44 ) ... was added into the cells and incubated for different times The results are summarized in Figure 4. 13A, Figure 4. 13C, and Figure 4. 13D 146 Chapter Role of S1P in human BM- and AD- MSCs proliferation ... P-P 44/ 42 α-tublin ctrl S1P5’ BSA5’ S1P10’ BSA10’ 4. 13B P-iκBα α-tublin ctrl S1P5’ ctrl S1P60’ S1P10’ S1P30’ S1P60’ BSA60’ 4. 13C P-P 44/ 42 α-tublin PTX+S1P60’ 4. 13D P-P 44/ 42 α-tublin Figure 4. 13...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 5

Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 5

... suggests the potential for the use of SPHK inhibitors in preconditioning stem cells before transplanting them into the body, by committing stem cells along specific differentiation path More work, including ... osteogenic differentiation in most of the cases, while at higher amounts (1μM DMS and 5 M CP6) showed to inhibit the osteogenic differentiation in most of the experiments Interestingly, in human BM- and ... differentiations pathways, to further validate and confirm the potential usage of SPHK inhibitors in stem cell differentiation, and S1P roles on stem cell proliferation 157 ...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 6

Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 6

... messenger in cell proliferation induced by PDGF and FCS mitogens." Nature 365 (64 46) : 557 -60 Olivera, Ana, Nicole Urtz (20 06) “IgE-dependent Activation of Sphingosine Kinases and and Secretion of Sphingosine ... N, N-dimethyl-derythro -sphingosine- induced apoptosis and signaling in U937 cells: independence of sphingosine kinase inhibition.” Prostaglandins & Other Lipid Mediators 86( 1-4):18-25 Kimura, T., ... (1999) "Sphingosine kinase expression increases intracellular sphingosine- 1-phosphate and promotes cell growth and survival." J Cell Biol 147(3): 545-58 Olivera, A and S Spiegel (1993) "Sphingosine- 1-phosphate...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation

Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation

... clinical usage Meeting this increasing need for more MSCs is one of the main motivations of the present study, in particular, in studying the role of S1P in adult stem cell proliferation Another ... maintenance and differentiation In particular, we are interested in the role of S1P in human adult stem cell proliferation and stemness maintenance, and how SPHK inhibition would affect human adult ... considering the laboratory interest in SPHK research and the above mentioned findings, my research project was derived and focused on studying the role of SPHK in adult stem cell differentiation, in...
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The role of microRNAs in embryonic stem cell development and differentiation

The role of microRNAs in embryonic stem cell development and differentiation

... as in embryonic carcinoma (EC) cells, which may have conserved roles in mammalian pluripotent stem cells (2) microRNAs expressed specifically in ESCs but not in other cells including EC cells These ... putative minimal promoter The number and precise sequences of the pre -microRNAs, their distance from the promoter and the polyadenylation sites, the regions flanking the hairpins, and the types, ... THE ROLE OF MICRORNAS IN EMBRYONIC STEM CELL DEVELOPMENT AND DIFFERENTIATION TAY MEI SIAN YVONNE B.Sc (Hons), NUS A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY NUS...
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Báo cáo y học:

Báo cáo y học: "Elevated expression of CD30 in adult T-cell leukemia cell lines: possible role in constitutive NF-κB activation" pps

... Retrovirology 2005, 2:29 Overexpression of CD30 in ATL cell lines We next examined CD30 expression in ATL-derived T -cell lines, HTLV-1 transformed cell lines and HTLV-1 negative T -cell lines using FACS ... binding protein pathways Virus Genes 2001, 22:279-287 Akagi T, Ono H, Shimotohno K: Expression of cell- cycle regulatory genes in HTLV-I infected T -cell lines: possible involvement of Tax1 in the ... stable expression of CD30 induces constitutive NF-κB activation in Rat-1 cells Exogenous stable expression of CD30 induces constitutive NF-κB activation in Rat-1 cells Rat-1 κB-bsrEGFPx2 cells...
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Báo cáo y học:

Báo cáo y học: "Inflammatory Signals shift from adipose to liver during high fat feeding and influence the development of steatohepatitis in mice" pot

... differences in severity of disease and times of disease onset depending upon the type of fats used for feeding Mice fed diets high in trans fats combined with high fructose in the drinking water ... RM, Yki-Järvinen H: Genes involved in fatty acid partitioning and binding, lipolysis, monocyte/macrophage recruitment, and inflammation are overexpressed in the human fatty liver of insulin-resistant ... both in adipose and liver under the influence of inflammatory signals [15,16] Furthermore, the reduction in gene activation observed in the epididymal adipose tissues at the later time point was...
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Báo cáo y học:

Báo cáo y học: "Inflammatory Signals shift from adipose to liver during high fat feeding and influence the development of steatohepatitis in mice" pptx

... differences in severity of disease and times of disease onset depending upon the type of fats used for feeding Mice fed diets high in trans fats combined with high fructose in the drinking water ... RM, Yki-Järvinen H: Genes involved in fatty acid partitioning and binding, lipolysis, monocyte/macrophage recruitment, and inflammation are overexpressed in the human fatty liver of insulin-resistant ... both in adipose and liver under the influence of inflammatory signals [15,16] Furthermore, the reduction in gene activation observed in the epididymal adipose tissues at the later time point was...
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Formulating business strategies for retail banking business of joint stock commercial bank for investment and development of Vietnam in the period of 2013 – 2017

Formulating business strategies for retail banking business of joint stock commercial bank for investment and development of Vietnam in the period of 2013 – 2017

... the theme: Formulating strategies for retail banking business of Joint Stock Commercial Bank for Investment and Development of Vietnam during the period 2013 - 2017 with the desire to apply ... for further development of the bank' s retail banking activities Subject and scope of the research: - Subject: Retail Activities at the Bank for Investment and Development of Vietnam - Scope of ... for the development of retail banking of BIDV in the period of 2013 - 2017 62 3.2.1 Summarization of the strategies from SWOT analysis 62 3.2.2 Selecting business strategies for...
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The development of an in vivo humanized mouse model to investigate epstein barr virus infection and tumorigenesis

The development of an in vivo humanized mouse model to investigate epstein barr virus infection and tumorigenesis

... overall incidence of PTLD is - 2% The incidence of the disease in renal and liver transplant is – 5%, in heart and heart-lung transplant is – 15%, and in intestinal transplant is 10 – 15% 125 The incidence ... from the EBNA3A, B, C family of proteins and the frequency of specific cells comprise Humanized mouse model of EBV infection Introduction up to 5% of total CD8+ T cells In the late stages of infection ... 25 Humanized mouse model of EBV infection Introduction Understanding EBV latency and persistence is the key to understanding the pathogenic role of this virus in associated lymphoid malignancies...
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FU LEI AND THE DEVELOPMENT OF CULTURE IN MODERN CHINA

FU LEI AND THE DEVELOPMENT OF CULTURE IN MODERN CHINA

... 55 Fu Lei would later meet with the Sun brothers in France and holiday together Jin Mei, p 72 56 ‘Faxing tongxin shiwu pian’ in Fu Lei wenji: wenyijuan, pp 23-77 The series was later collated in ... Lee, ‘Incomplete Modernity: Rethinking the May Fourth Intellectual Project’ and Rudolf G Wagner, The Canonization of May Fourth’ in Milena Dolezelova-Velingerova and Oldrich Kral, eds., The Appropriation ... ‟ Fu Lei, ‘Letter to Romain Rolland, March 1934’, in Fu Lei wenji: shuxinjuan (Beijing: Dangdai shijie chubanshe, 2006), pp 462 – These were Michelangelo and Tolstoy, published in 1935 by the...
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The role of language in adult education and poverty reduction in Botswan

The role of language in adult education and poverty reduction in Botswan

... program in Botswana maintains the hegemony and the gap between the poor and the rich, the major and minority groups In order to redress poverty the adult education program needs to be aware of the social ... vital in fighting poverty The adult education program can thus harness the local languages and indigenous knowledge of the minority and poor people and use them to mobilize the poor and minorities ... the learners to be confident to participate in the discussions and activities of their education and economy This involves the teaching of local languages, Setswana and English By involving the...
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Từ khóa: fertilization and development of embryo in humantraining and development of managers in international businessdevelopment of balancing in the internal electricity market in europepromoting fostering and development of statistics in developing countriesthe relevance of social determinants of health in the development of physiotherapy in the home environmentthe development of testis in sheepmtx cf101 and mtx cf101 treatment on development of aia in ratsthe development of gis in local governmentdevelopment of embryo in humans videodevelopment of embryo in humans pptdevelopment of embryo in humans animationrole of oxidative stress in development of complications in diabetesinhibitors of hemopoietic stem cell proliferationclinical and laboratory aspects of preimplantation genetic diagnosis and derivation of affected human embryonic stem cell lineserk1 2 and of stat5 in tki resistant cell linesBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Nghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngThơ nôm tứ tuyệt trào phúng hồ xuân hươngBT Tieng anh 6 UNIT 2Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtNguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ