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Proline rich acidic protein 1 in life and death 1

Proline rich acidic protein 1 in life and death 2

Proline rich acidic protein 1 in life and death 2

... 71 2 .10 .2 .1 Labeling of protein with Alexa Fluor dye 71 2 .10 .2. 2 Bacteria binding assay using labeled protein 71 2 .11 DNA damage assay 72 2 .11 .1 Alkaline single-cell ... 14 1 3 .11 .2 PRAP1 interacts with Hsp 70 14 1 3 . 12 Role of PRAP1 in apoptotic cells 14 5 3 . 12 .1 Induction of PRAP1 expression in apoptotic cells 14 5 3 . 12 .2 PRAP1 binds to ... 40 2 .1. 1 Cell lines 40 2 .1. 1 .1 PBMCs 40 2 .1. 1 .2 Cell culture 41 2 .1. 2 Treatment of cells 41 2 .1. 2 .1 Treatment of human colon cancer cells HT 29 ...
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Proline rich acidic protein 1 in life and death 3

Proline rich acidic protein 1 in life and death 3

... 76 3 .1 PRAP1 and intestinal differentiation 3 .1. 1 PRAP1 is expressed in epithelial cells of the intestines The expression of PRAP1 in human intestinal tract was studied using immunohistochemistry ... 77 Figure 3 .11 PRAP1 is expressed in the epithelial cells of small intestine Representative figure showing PRAP1 immunohistochemical staining in normal human small intestine tissue (X100 magnification) ... treatment, and further increased to 5-fold at 72 hours (Figure 3 .18 -B) These data indicate that induction of PRAP1 occurred by an increase of PRAP1 mRNA 83 Figure 3 .17 PRAP1 expression is induced...
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Proline rich acidic protein 1 in life and death 4

Proline rich acidic protein 1 in life and death 4

... (p53-/-) and one with p 21 knockout (p 21- /-) PRAP1 mRNA was induced in both HCT 11 6 wild-type and p 21- /- cell lines, but not in p53-/- 10 6 Figure 3.37 Early upregulation of PRAP1 in a dose- and time-dependent ... 5-FU induced an increase in cyclin E1 and cyclin A1 protein levels, reflecting an arrest at S-phase (Figure 3.55) Repression of PRAP1 induction by 5FU caused a significant reduction in cyclin A1 ... function of p 21 in causing cell cycle arrest These 12 5 Figure 3.55 Repression of PRAP1 expression reduces cyclin A1 Representative western blot of cyclin E1, cyclin A1, p53 and B-ACTIN in HCT 11 6 cells...
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Proline rich acidic protein 1 in life and death 5

Proline rich acidic protein 1 in life and death 5

... Identification of PRAP1 binding protein by MS-MS Representative figure showing the identification of PRAP1 binding proteins after performing MS-MS Figure 3. 71 PRAP1 physically interacts with Hsp ... F-actin in HCT 11 6 cells stably expressing PRAP1 (PRAP1) and its empty vector control stable cell line (Vector), or after transfection of control siRNA and PRAP1 siRNA for 72 hours F-actin was ... was stained using Palloidine conjugated to FITC (Green) and nuclear was counter stained with Hoechst (Blue) 14 1 3 .11 .1. 3 Effects of PRAP1 on microtubules To further study the effect of PRAP1 on...
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Proline rich acidic protein 1 in life and death r6

Proline rich acidic protein 1 in life and death r6

... DISCUSSION 17 3 4 .1 Role of PRAP1 in differentiated epithelial cells 4 .1. 1 Regulation of PRAP1 by differentiation 4 .1. 1 .1 Expression of PRAP1 in intestinal epithelium The proline- rich acidic protein (PRAP1) ... floating cells, we employed the annexin V-FITC staining assay Annexin V is a phospholipid binding protein that has a high binding affinity for phosphatidylserine (PS) that translocates from inner ... potent in inducing PRAP1 expression in U937, THP -1 and Jurkat (Figure 3.90) We failed to detect any significant induction of PRAP1 by 5-FU in HL-60 cells PRAP1 expression was also induced by CPT and...
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Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 1

Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 1

... 3 .1 Mia1p in assembly and maintenance of persisent iMTOCs at nuclear envelope 44 3 .1. 1 Lack of microtubule binding protein Mia1p results in fewer interphase Microtubules 44 3 .1. 1 .1 Mia1p is a microtubule ... cytoskeleton 18 18 1. 2.4 .1. 1 Architecture and dynamics of interphase microtubule cytoskeleton 18 1. 2.4 .1. 2 Functions of interphase microtubule cytoskeleton 19 1. 2.4 .1. 2 .1 Maintaining cell morphology 19 1. 2.4 .1. 2.2 ... between Mia1p and Alp14p 10 9 4.5 .1 Mia1p interacts with Alp14p but not Dis1p 10 9 4.5.2 Different aspect of Mia1p and Alp14p functions in microtubule x attachment to the NE 11 0 4.5.3 Lack of Mia1p and...
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Báo cáo Y học: Expression of uncoupling protein-3 in subsarcolemmal and intermyofibrillar mitochondria of various mouse muscle types and its modulation by fasting docx

Báo cáo Y học: Expression of uncoupling protein-3 in subsarcolemmal and intermyofibrillar mitochondria of various mouse muscle types and its modulation by fasting docx

... determined by comparing the level of COX protein in the sum of the two mitochondria populations with that in the homogenate, was 88% Expression of UCP3 protein in SS and IMF mitochondria of various ... and soleus muscles Hesselink et al [23], who studied the distribution of UCP3 protein in various human muscle types by immunofluorescence, showed a higher expression of UCP3 protein in glycolytic ... phosphorylation for ATP synthesis, i.e the soleus muscle, contains less UCP3 per mg of mitochondrial protein than glycolytic muscle types and (b) in all muscle types the mitochondria most closely associated...
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báo cáo khoa học:

báo cáo khoa học: " Storage protein profiles in Spanish and runner market type peanuts and potential markers" pps

... fastigiata fastigiata Spanish Spanish Spanish Spanish Spanish Spanish Runner runner runner runner runner runner China China China China China India USA USA USA USA USA USA [7-9], indicating that very ... polypeptides and the basic polypeptides [17] The uniformity of the one-dimensional SDS-PAGE protein profiles within the runner type and Spanish type cultivars and breeding lines is in agreement ... seed total protein profiles 2-D SDS-PAGE peanut seed total protein profiles Two-dimensional SDS-PAGE of peanut seed total protein profiles of cultivated peanut genotypes, Spanish market type Gels...
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Báo cáo y học:

Báo cáo y học: "GC- and AT-rich chromatin domains differ in conformation and histone modification status and are differentially modulated by Rpd3" doc

... Given the differences in chromatin conformation and transcriptional activity of GC- and AT-rich chromatin domains, we hypothesized that these domains may also display differences in histone modification ... that out of 11 modifications (histone H4 Lys8 (H4K8) and Lys12 (H4K12), and histone H3 Lys9 (H3K9) and Lys18 (H3K18)) are enriched in GC-rich chromatin and depleted in AT-rich chromatin The levels ... AT-rich chromatin in rpd3Δ cells; rpd3Δ-GC, GC-rich chromatin in rpd3Δ cells; WT-AT, AT-rich chromatin in wild-type cells; WT-GC, GC-rich chromatin in wild-type cells obtain a good fit, implying...
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