Characterisation of the BRCT DBL protein ECT2 in cell cycle regulation
Characterisation of the BRCT DBL protein ECT2 in cell cycle regulation
... Isolation of the Ect2 proto-oncogene 37 2.2 Cell cycle- dependent regulation of Ect2 37 2.3 N-terminal domains of Ect2 are similar to cell cycle regulatory proteins 41 2.4 Cellular functions of Ect2 ... Review during interphase and decrease during mitosis while the levels of cyclin E remain constant throughout the cell cycle By re-introducing recombinant proteins into cyclindepleted cell extracts, ... Disruption of Ki-67 function by micro-injecting antibodies results in delay of the cell cycle [11] Expression of the protein is regulated throughout cell cycle and it is absent in quiescent cells, making...
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Báo cáo khoa học: Fluorescence studies of the replication initiator protein RepA in complex with operator and iteron sequences and free in solution pdf
... Fluorescence studies of RepA R E M Diederix et al Interestingly, in the latter case, the protein binds as a monomer [26] Free in solution, the protein is essentially dimeric, ... DNA-binding domains, and rearrangement of the relative orientation of the two domains [7,9] The conformational change upon iteron binding may expose a recognition site for proteinprotein interaction, ... latter protein, the crystal structures of both the monomeriteron and dimeroperator complexes are available, indicating secondary structural changes in the linker connecting the dimerization and...
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Báo cáo khoa học: Increased sensitivity of glycogen synthesis to phosphorylase-a and impaired expression of the glycogen-targeting protein R6 in hepatocytes from insulin-resistant Zucker fa ⁄ fa rats pptx
... contributes to the hepatic defect in the Zucker fa ⁄ fa rat 1990 Results High activities of glucokinase and phosphorylase in hepatocytes from fa ⁄ fa rats Hepatocytes from fa ⁄ fa rats had a higher total ... overexpression in hepatocytes [26– 28] However, the contribution of these targeting proteins to the increased activity of glycogen synthase phosphatase in hepatocytes from Zucker fa ⁄ fa rats ... strength of phosphorylase on glycogen synthesis in hepatocytes from Wistar rats [16,17] or for the impaired glycogen synthesis in hepatocytes from Zucker fa ⁄ fa rats, which not have diminished glycogen...
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báo cáo khoa học: " In vivo imaging of the tonoplast intrinsic protein family in Arabidopsis roots" ppsx
... present in the tissues lacking TIP1;1 (Fig 2) It is therefore possible that these remaining isoforms compensate for the lack of TIP1;1 in the knockout On the other hand, the effect of the absence of ... expression of TIP3;1 and TIP1;1 in Arabidopsis and found minimal overlap in the timing of their expression, with TIP3;1 being abundant in embryos of mature seeds and sharply declining during seed germination, ... Weig AR, Kjellbom P: The complete set of genes encoding major intrinsic proteins in Arabidopsis provides a framework for a new nomenclature for major intrinsic proteins in plants Plant Physiol...
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báo cáo khoa học: " Characterisation of the tryptophan synthase alpha subunit in maize" pptx
... Site-specific mutagenesis of the alpha subunit of tryptophan synthase from Salmonella typhimurium Changing arginine 179 to leucine alters the reciprocal transmission of substrateinduced conformational ... scheme of the tryptophan synthase reaction General General scheme of the tryptophan synthase reaction Indole, which is formed from IGP by the α-subunits is channelled to β-subunits, which synthesize ... Frey M, Gierl A, Niks D, Dunn MF, Schlichting I: On the structural basis of the catalytic mechanism and the regulation of the alpha subunit of tryptophan synthase from Salmonella typhimurium and...
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Bóa cáo y học: " Identification and characterisation of the high-risk surgical population in the United Kingdom" pps
... involved in data analysis and drafting the manuscript, and approved the final version All authors had full access to data and take responsibility for the integrity of the data and the accuracy of the ... duration of the ICU stay was 1.1 (0.8–2.4) days and the duration of hospital stay was 15 (10–26) days For emergency ICU admissions the duration of the ICU stay was 2.1 (0.9–5.6) days and the duration ... [13,14]; however, in the absence of data describing the size of the high-risk population, it is difficult to convince clinicians and managers either of the need to introduce new therapeutic approaches...
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characterisation of the acto-myoa motor complex in toxoplasma gondii
... G-actin binding proteins like ADF/cofilin, CAP, and profilin, are crucial for keeping actin in the monomeric form While P falciparum has two ADF/cofilin isoforms, most other Apicomplexa like T .gondii ... and invasion machinery of T gondii: The MyoA motor complex consists of MyoA, MLC1 and the IMC interacting proteins GAP40, GAP45, and GAP50 Together the MyoA motor complex is connected to F- actin, ... subdomains form the ATP binding pocket, the barbed and pointed end of the monomer and serve as the binding site for actin-binding proteins Arps have a high homology to conventional actin with the...
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Mutational analysis of the TATA binding protein (TBP) in saccharomyces cerevisiae
... ubiquitin attached to Protein X and the Cterminus to Protein Y and the reporter RUra3p 47 Figure 2.8 Interaction between Protein X and Protein Y Interaction of the two proteins brings about the ... able to interact with TFIIF more rapidly, resulting in strong activation of transcription (Choi et al., 2003) 2.5.1.3 TFIID /TATA- Binding Protein The TATA- binding protein, Tbp1p, binds to the TATA- box ... transcription by initiating the recruitment of limiting factors at the promoter The raising of the local concentrations of these limiting factors at the promoter brings about the recruitment of other transcription...
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Functions of the dynamin like protein VPS1 in actin organization in saccharomyces cerevisiae
... FUNCTIONS OF THE DYNAMIN- LIKE PROTEIN VPS1 IN ACTIN ORGANIZATION IN SACCHAROMYCES CEREVISIAE YU XIANWEN (B.Sc., XIAMEN UNIVERSITY) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY INSTITUTE ... PRD, proline arginine rich domain (B) The domain structure of dynamin- like proteins 1.3.1.1 Domains and properties of dynamins Dynamins are different from the small GTPases in that they are large, ... tyrosine kinases The binding between dynamins and these signaling proteins can be stimulated upon growth factor treatments, indicating the involvement of dynamin in cellular signaling pathway The...
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The Retinoblastoma Gene Family in Cell Cycle Regulation and Suppression of Tumorigenesis
... on the role of the retinoblastoma gene family in cell cycle regulation and tumor suppression The pRb Cell Cycle Control Pathway: Components and the Cancer Connection The retinoblastoma protein, ... over-expression of D-type cyclins, mutations rendering Cdk4 The Retinoblastoma Gene Family 185 Fig The p16INK4A -pRb and the p19ARF -p53 pathway involved in cell cycle progression and tumorigenesis Components ... will be used The Retinoblastoma Gene Family 187 The Retinoblastoma Gene Family 4.1 Rb Gene Family Members The retinoblastoma gene family comprises, besides Rb, the structurally and functionally...
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Elucidation of the physiologic role of TRIP br2 in cell cycle regulation and cancer pathogenesis
... 2000) CDK protein levels remain stable during the cell cycle, in sharp contrast to their activating proteins, the cyclins The oscillatory expression of most cyclin proteins during cell cycle progression ... C-terminus of TRIP- Br2, which includes the putative C-terminal NES of TRIP- Br2, stabilized TRIP- Br2 in G2/M phase 189 Figure 30E Inhibition of nuclear export of TRIP- Br2 by LMB restores its stability in ... include the cyclin-dependent kinases (CDKs), a family of serine/threonine protein kinases that are activated at specific points of the cell cycle To date, nine CDKs have been identified and, of these,...
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Tài liệu Báo cáo khoa học: Single phosphorylation of Tyr304 in the cytoplasmic tail of ephrin B2 confers high-affinity and bifunctional binding to both the SH2 domain of Grb4 and the PDZ domain of the PDZ-RGS3 protein ppt
... that the phosphorylation of Tyr304 confers highaffinity and specific binding of ephrinB2(301–333) to the Grb4 SH2 domain The ephrinB2(301–333)-pY304 peptide can bind to the Grb4 SH2 and RGS3 PDZ domains ... of Grb4 binds to the cytoplasmic tail of ephrin B2 in a phosphorylation- dependent manner It is therefore interesting to know whether Grb4 and PDZ- RGS3 can bind to the cytoplasmic tail of ephrin ... experiments in the absence of the GST -PDZ protein (Fig 6B) Therefore, it appears that binding of the PDZ domain to the ephrinB2(301–333)-pY304 peptide has no impact on the binding of the Grb4 SH2 domain...
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Tài liệu Báo cáo khoa học: Co-operative effect of the isoforms of type III antifreeze protein expressed in Notched-fin eelpout, Zoarces elongatus Kner ppt
... containing the isoforms of nfeAFP 483 Co-operative effect of type III AFP isoforms Y Nishimiya et al A B Fig (A) HPLC purification of the isoforms of type III AFP from Notched-fin eelpout The peaks ... QAE isoforms of nfeAFP, whereas the others correspond to SP isoforms, and the TH activity of the QAE isoform is higher than that of the SP isoform 487 Co-operative effect of type III AFP isoforms ... Val9-containing isoform (QAE2 group); nfeAFP13, the largest isoform containing 485 Co-operative effect of type III AFP isoforms Y Nishimiya et al Fig Alignment of amino acid of sequence of type III...
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Báo cáo khoa học: Mass spectrometric characterization of the covalent modification of the nitrogenase Fe-protein in Azoarcus sp. BH72 ppt
... allowed the identification of the arginine-specific Azoarcus Fe-protein ADP-ribosylation ADP-ribosylation on the nitrogenase Fe-protein in the b-proteobacterium Azoarcus sp strain BH72 Our approach involved ... post-translational modifications on nitrogenase Fe-proteins or other proteins Results and Discussion Site-directed mutagenesis of the target arginine of dinitrogenase reductase An indication for the covalent modification ... ADP-ribosylation of nitrogenase Fe-protein on a specific arginine residue in these bacteria This suggested that nitrogenase Fe-protein was modified by ADPribosylation of R102 also in Azoarcus sp BH72 Further...
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Báo cáo khoa học: Identification of multiple isoforms of the cAMP-dependent protein kinase catalytic subunit in the bivalve mollusc Mytilus galloprovincialis potx
... mussel M galloprovincialis Results Separation of different isoforms of the C -subunit In order to demonstrate the presence of different isoforms of the PKA C -subunit in mussels, the protein was ... isoforms [33] In summary, in this work we demonstrate the presence of several structurally different isoforms of the PKA C -subunit in mussel tissues In principle, the combination of these catalytically ... tissuespecific protein kinase (Cc) from human testis – representing a third isoform for the catalytic subunit of cAMP-dependent protein kinase Mol Endocrinol 4, 465–475 22 Gross RE, Bagchi S, Lu X & Rubin...
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