... rationally Furthermore, regions could be identified in the enzyme that are clearly important for amylosucrase activity, as b strand 7, connecting to the important Asp393 residue, and the region close ... wild-type, activity at elevated temperatures had increased drastically for variant E9, which indicates increased stability In contrast, variant H4, which was not selected for increased thermostability, ... incubating at 95 °C for min, adding 60 lL of this mixture to 180 lL H2O, and measuring the absorbance at 540 nm The formation of the amylose-type polymer was Molecular evolution to improve amylosucrase...