Understanding cofactor f420 dependent mechanism(s) in the activation of bicycle nitroimidazoles and in the physiology of mycobacterium tuberculosis

... reduction of the cofactor F420H2 is the active form of the cofactor that is utilized by Ddn (Figure 1.3) The physiological role of both cofactor F420 and the activating enzyme Ddn in Mtb is unknown ... described in this thesis Before detailing the objectives of the thesis in section 1.6, the following is a brief description of the ten compounds in the pipeline 15 A B Figure 1.2 The Global Pipeline ... developed Binding affinity of PA-824 to Ddn was monitored through intrinsic fluorescence quenching of the protein facilitating an enzymatic turnover-independent assessment of affinity The results...

Báo cáo khoa học: Molecular dissection of the biosynthetic relationship between phthiocerol and phthiodiolone dimycocerosates and their critical role in the virulence and permeability of Mycobacterium tuberculosis doc

... postinfection, we observed a marked difference between the number of CFUs of the wild-type strain and that of the PMM56 mutant strain in lungs and in the spleen (Fig 7A) Indeed, both strains ... cell envelope of M tuberculosis and to virulence [3–6] Nevertheless, their precise molecular mechanisms of action are still unknown, and the speciﬁc roles of the various members of the DIM family, ... DIM A and DIM B, in these functions have never been investigated The recent developments in our understanding of the biosynthetic pathway of DIMs have provided the means to investigate the contribution...

Báo cáo khoa học: Kinetic and mechanistic characterization of Mycobacterium tuberculosis glutamyl–tRNA synthetase and determination of its oligomeric structure in solution pptx

... the discriminating behaviour of Mt-GluRS in E coli Studies on Tt-GluRS indicated that discriminating and nondiscriminating GluRS can be distinguished on the basis of the presence of a specic ... Analysis of the kinetics of proteolysis of Mt- and Ec-GluRS Fig S9 Minimal models of the proteolytic events leading to fragments M1M5 of Mt-GluRS and E1-E3 of Ec-GluRS Fig S10 DLS analysis of GluRS ... N-terminal sequence and the mass of the fragments, and from the kinetics of the process (Figs S7S9 and Table S5) we concluded that the main sites of proteolytic cleavage are at the C-terminus of...

Báo cáo khoa học: The sigma factors of Mycobacterium tuberculosis: regulation of the regulators potx

... provided evidence of the interplay of r factors and their interaction with regulators, how exactly these factors coordinate with one another in the face of an adverse event, and the order of events, ... replication in the lung during the short-term infection of mice [109,110], the negative regulation of their synthesis by rM negates the possibility of its role in virulence during the early course of infection ... in the sigG mutant The aforementioned result is expected in view of the downregulation of sigH and the upregulation of sigD upon the deletion of sigG in M tuberculosis [90] This ﬁnding further...

Báo cáo Y học: Trehalose-phosphate synthase of Mycobacterium tuberculosis Cloning, expression and properties of the recombinant enzyme pdf

... important and probably essential function in the physiology of many organisms The most widely demonstrated pathway for the synthesis of trehalose involves two enzymes that catalyze the following ... amounts of the recombinant TPS After an incubation of 15 min, one set of incubation mixtures was assayed by the anthrone colorimetric method and the other set was assayed by the coupled enzyme assay ... debranching enzyme, and maltooligosyl trehalose synthase These genes show 40% homology to regions in the genome of M tuberculosis [41] However, whether either of these two pathways are actually utilized...

Báo cáo khoa học: "Random amplified polymorphic DNA (RAPD) analysis of Mycobacterium tuberculosis strains in India" docx

... eht yb desuac raef dna ,]23,61[ gnildnah yb noissergga lacisyhp dna gnituohs dna ,]83,92,02[ slamina rof ytlevon a si nep gnildnah ehT sserts lanoitidda esopmi ]32,71,61,9[ gnildnah gnirud elpoep ... ,641-321.pp erafleW laminA rof snoitacilpmI dnA selpicnirP cisaB :ssertS slaminA fo ygoloiB ehT ).sde( AJ hcneM ,PG grebroM :nI sserts fo tnemerusaem ffo-sdnah dna no-sdnaH RL ,swehttaM ,RJ margnI ... rof noitnetnoc knurt a ni lamina hcae gniniartser dna ,yad gnilpmas eht fo gninrom eht ni larroc a ot slamina gnidael yb demrofrep saw hcihw ,noitazilibommi retfa ht06 dna ,ht54 ,ht83 ,ht13 syad...

Báo cáo y học: "Glutathione and growth inhibition of Mycobacterium tuberculosis in healthy and HIV infected subjects" pot

... from healthy and HIV- infected subjects Interestingly, in HIV subjects, H37Rv infection induced the blood cultures to produce increased levels of pro-inflammatory cytokines such as IL-1, TNF-α, and ... effects of GSH in growth control of H37Rv in blood cultures derived from healthy and Figure HIV- infected subjects Model describing direct and indirect effects of GSH in growth control of H37Rv in ... assay of IL-10 Cytokine assay was performed using a Beadlyte kit procured from Upstate Data in Figure 5a and b are averages from four healthy and five HIV- infected subjects, respectively rapidly...

Báo cáo khoa học: Thyroid hormone induces the expression of 4-1BB and activation of caspases in a thyroid hormone receptor-dependent manner pptx

... designated HeLaTR /4-1BB and HeaLaTR/ TRAF1, respectively Primers for amplifying the 4-1BB and TRAF1 cDNAs were: 5¢-GAATTCAAGCTTATGGGA AACAGCTGTTACAACATA-3¢ and 5¢-GAATTCAAG CTTCACAGTTCACATCCTCCTTCTTCT-3¢ ... used for amplifying the hTRa1 cDNA were: 5¢-CCCGGGAAGCTTCGGACCATGG AACAGAAGCCAAGCAAGGTG-3¢ and 5¢-CCCGGG GTCGACGACTTCCTGATCCTCAAAGACCTC-3¢ In order to overexpress 4-1BB and TRAF1 in the HeLaTR cells, ... immunoprecipitated from the cell extracts with an antihuman TRa Ig The human TRa1 protein was fractionated on a polyacrylamide gel and visualized by an image analyzer The arrow indicates the band of human...

Báo cáo khoa học: "Circadian pattern of activation of the medical emergency team in a teaching hospita"

... loudspeakers and paging system, and a detailed log of all calls is maintained Criteria for medical emergency team activation Calling criteria for our MET service are based on acute changes in heart ... drugs and defibrillators Outcome measures Information on the activation of all MET calls is maintained on a hospital switchboard logbook that includes the date and time of the call, as well as the ... per year Hospital emergency response teams The acute care hospital has two levels of medical emergency responses and teams A traditional cardiac arrest ('code blue') team is comprised of a cardiology...

Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf

... et al MT1-MMP and furin can interact with GRASP55 Overexpression of GRASP55 has been found to increase the amount of complex containing MT1MMP and furin, and the expression of a catalytically inactive ... with a full-length furin cDNA, were permeabilized and stained with polyclonal antibodies against furin and GRASP55 Arrows show examples of membrane compartment containing GRASP55 and furin Scale ... general mechanism for furin- mediated activation of transmembrane substrates The activation of ADAM17 by furin [68] and our observation of an interaction between GRASP55 and the ICD of ADAM17...

Tài liệu Báo cáo khoa học: Protein kinase CK2 activates the atypical Rio1p kinase and promotes its cell-cycle phase-dependent degradation in yeast pdf

... aprotinin, leupeptin and pepstatin, twice in kinase buffer, and used for in vitro kinase assays In vitro kinase assays Recombinant human protein kinase CK2 holoenzyme (0.5 U, corresponding to ... orthologues occur in higher eukaryotes) [13] Rio1 protein kinase is regulated by CK2 The biological role of Rio1p or even the pathways in which the Rio1 protein kinase is involved are far from being understood ... (2002) Joining the cell survival squad: an emerging role for protein kinase CK2 Trends Cell Biol 12, 226–230 Meggio F & Pinna LA (2003) One-thousand -and- one substrates of protein kinase CK2? FASEB...

Báo cáo khoa học: A simple in vivo assay for measuring the efﬁciency of gene length-dependent processes in yeast mRNA biogenesis doc

... that measurement of acid phosphatase activity was the best estimation of the mRNA abundance in our systems, and from here on we use the ratio of acid phosphatase activities as an indicator of gene ... tested the consistency of the GLAM ratios by performing the assay in a wide set of previously characterized mutants involved in mRNA biogenesis The GLAM ratios were always calculated for two ... caused on the minimal PHO5 mRNA, therefore producing similar ratios in the wild type and in the mutant strain (Fig 3C) Our results conﬁrm that TFIIS is not playing a general role in mRNA biogenesis...

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