Screening and evaluation of the anticancer potential of scorpion venoms and snake venom l amino acid oxidase in gastric cancer

... SCREENING AND EVALUATION OF THE ANTICANCER POTENTIAL OF SCORPION VENOMS AND SNAKE VENOM L- AMINO ACID OXIDASE IN GASTRIC CANCER DING JIAN (B.S.c) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... responsible for the anticancer activities of BHV and LAAO The novel findings shed light on the development of anticancer agents from scorpion venoms and L- amino acid oxidases, and provided biological ... cell adhesion is absent so that the individual cell can infiltrate and thicken the stroma wall The development of intestinal type gastric cancer normally involves sequential histopathological...

Báo cáo khoa học: Identiﬁcation of an antibacterial protein as L-amino acid oxidase in the skin mucus of rockﬁsh Sebastes schlegeli pot

... that the antibacterial protein purified from the skin mucus of S schlegeli (SSAP) is a 120-kDa glycoprotein, being a new member of the l-amino acid oxidase (LAO) Fig Purification of antibacterial protein, ... because of the scarcity of the protein of interest In this study therefore we isolated the antibacterial protein, SSAP, from the skin mucus of S schlegeli by a combination of three different types of ... importance of the antibacterial protein as a primary innate immune strategy in the rockfish skin In a previous study [22], we obtained the antibacterial protein by lectin affinity column chromatography and...

Báo cáo khoa học: Novel L-amino acid oxidase with antibacterial activity against methicillin-resistant Staphylococcus aureus isolated from epidermal mucus of the ﬂounder Platichthys stellatus pptx

... antibacterial protein in the epidermal mucus of the ﬂounder Platichthys stellatus This species, which has a rich covering of mucus on its body surface, inhabits brackish water at the mouths of ... the antibacterial component of PSEM is a protein Puriﬁcation of antibacterial protein from PSEM Fig Antibacterial activity of PSEM against (A) Staphylococcus epidermidis, (B) Staphylococcus aureus ... The present communication describes the isolation and cloning of this LAAO-like antibacterial protein from P stellatus Results Antibacterial activity of mucus It is assumed that Platichthys stellatus- derived...

Báo cáo khoa học: Two L-amino acid oxidase isoenzymes from Russell’s viper (Daboia russelli russelli) venom with different mechanisms of inhibition by substrate analogs pdf

... Mixed inhibition of L2 by NATA with initial competitive inhibition suggests that NATA binds at two different sites, of which the substrate- binding site has higher afnity Inhibition by NAT Inhibition ... stability However, the inhibition proles of the substrate analogs NATA, NAT and OAB in the presence of l-Phe as substrate indicated that the two isoforms were inhibited by different mechanisms A detailed ... phospholipase A2 from Russells viper venom J Chromat B 845, 232243 18 Mandal S & Bhattacharyya D (2007) Ability of a small, basic protein isolated from Russells viper venom (Daboia russelli russelli)...

Báo cáo khoa học: A new highly toxic protein isolated from the death cap Amanita phalloides is an L-amino acid oxidase pdf

... it was suggested that amine oxidases are enzymes of cellular amino acid catabolism, comprising potential candidates for a mechanism that catalyses nitrogen mineralization from amino acids at the ... organism In conclusion, in the present study, a novel cytotoxic protein was isolated from the death cap and characterized The physico-chemical, chemical and biological characteristics of this protein ... new L-amino acid oxidase from Daboia russellii siamensis venom Toxicon 54, 763–771 Zhang L & Wu WT (2008) Isolation and characterization of ACTX-6: a cytotoxic l-amino acid oxidase from Agkistrodon...

The mechanistic studies of the anticancer potential of artesunate in human cancer cells

... Nanyang Technological University, Singapore (Silver Best Poster Award) vi THE MECHANISTIC STUDIES OF THE ANTICANCER POTENTIAL OF ARTESUNATE IN HUMAN CANCER CELLS Table of Contents DECLARATION ... ART induced autophagy in human cervical cancer HeLa cells evidenced by the increase of autophagic flux In the search of the mechanisms for ART-induced autophagy, we found that ART inhibits mechanistic/ mammalian ... eIF4E binding protein beclin-1-regulated autophagy AMP activated protein kinase artesunate bafilomycin A1 Bax-interacting factor big mitogen-activated protein kinase bovine serum albumin cycloheximide...

Studies of the anticancer potential of andrographolide in human cancer cells

... the importance of PT in the mitochondrial apoptosis, because in the absence of the hallmarks of PT, including the loss of the inner membrane potential and the rupture of the outer membrane, the ... hypoxia), and they found that the type, strength and kinetics of the target gene profiles depend on the levels of p53 in the cell, the type of inducing agent, and the cell type investigated (Wang ... suggesting the anticancer potential of this compound However, the molecular mechanisms are largely unknown Therefore, in order to systematically investigate the anticancer properties of Andro, the...

Tài liệu Báo cáo khoa học: Implication of the glutamine synthetase ⁄glutamate synthase pathway in conditioning the amino acid metabolism in bundle sheath and mesophyll cells of maize leaves doc

... serine and glycine was reduced from 33% in the light to 25% in the dark (Fig 7A) Glutamine and asparagine were the major amino acids in the xylem sap in both the light (63% of the total amino acids) ... 3195 Amino acid metabolism and translocation M.-H Valadier et al Table Amino acid composition in leaves, and amino acid percentage ratio in the phloem exudates and leaves and xylem bleeding sap and ... molecular signalling in the expression of the genes and encoded enzymes involved in nitrate assimilation and amino acid synthesis [7] The stalk and leaves below and above the ear act as the source...

Tài liệu Báo cáo khoa học: Local stability identiﬁcation and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf

... et al Staphylococcal nuclease refolding investigated Two point-mutated proteins, with a single base substitution of alanine for tryptophan (W14 0A) and alanine for lysine (K13 3A) , and two truncated ... both the wild-type protein and mutants E142O and K13 3A Thermal analysis of protein unfolding The DSC curves of the wild-type protein and the mutants K13 3A, E142O, W14 0A and W140O are shown in Fig ... Our CD and DSC data show that the W140 in SNase is the amino acid responsible for the stability of the whole protein However, in comparison with the wild-type protein, the mutant W14 0A retains signiﬁcant...

Báo cáo khoa học: Local stability identiﬁcation and the role of key acidic amino acid residues in staphylococcal nuclease unfolding ppt

... Local stability and key acidic amino acids in staphylococcal nuclease by the interactions of E75 with H121 and K97 It is believed that a small number of key amino acid segments play major roles ... 44% of E129G), respectively, lower DHcal values than the wild-type protein These outcomes indicate that little in uence is exerted by Gly 3969 Local stability and key acidic amino acids in staphylococcal ... hypothesize that pH unfolding SNase is mainly processed by the addition of 2.5 protons and both E75 and E129 are the targets of protonation It is of interest to determine which other amino acids...

Báo cáo khoa học: Structural and biological effects of a b2- or b3-amino acid insertion in a peptide Application to molecular recognition of substance P by the neurokinin-1 receptor ppt

... control at t ¼ for each peptide The percentage of degradation was calculated by comparing the area of the peaks of the intact peptide at t ¼ and t ¼ 60 Binding assays Binding assays were carried ... [33], the peptides containing a b-amino acid substitution in position have increased stability compared to the corresponding a- amino -acid- containing peptides Therefore it is possible to increase peptide ... acid incorporation When Phe7 or Phe8 are replaced by b2-HPhe, the corresponding analogues are weak competitors of speciﬁc NK-1 binding sites These amino acids are in the helical domain of SP which...

Báo cáo khoa học: Structural and functional consequences of single amino acid substitutions in the pyrimidine base binding pocket of Escherichia coli CMP kinase pdf

... with the amino group and the N3 atom of the cytosine (Fig 1) This study uses site-directed mutagenesis to further explore the contribution of these amino acids interacting with the pyrimidine ring ... consisting of three domains, the CORE, the LID and the NMPbind [1] A characteristic of bacterial CMP kinases is an extension of the NMPbind domain by 40 amino acid residues forming a three-stranded ... NMPs (A37 and T39 in these enzymes are equivalent to S36 in E coli CMP kinase) Substitution of R110 with Met in E coli CMP kinase affected both kcat and Km The kcat Km ratio with CMP and dCMP decreased...

Báo cáo khoa học: On the reaction of D-amino acid oxidase with dioxygen: O2 diffusion pathways and enhancement of reactivity docx

... larger than the maximal O2 concentration in solution) [6–9] These enzymes appear to ‘consume’ O2 without highaffinity binding Because of the scarcity of information concerning the reaction of flavoproteins ... in the oxidized form, and the spectrum of the reduced enzyme is observed only at the end of the observation time, i.e when the O2 concentration becomes very low (Fig 2A and Fig S3) This is consistent ... higher using the E-Flred–IA complex (Fig 4B and Tables and 2) Modulation of O2 reactivity in D-amino acid oxidase Functional data on flavo -oxidase variants designed on the basis of molecular dynamic...

Báo cáo khoa học: Inhibition of PI3K/Akt partially leads to the inhibition of PrPC-induced drug resistance in gastric cancer cells pdf

... of PrPC-induced cell drug resistance in gastric cancer cells PI3K/Akt is involved in the activation of P-gp by PrPC in gastric cancer To further investigate the underlying mechanism of PI3K/Akt- mediated ... signiﬁcantly increased The results indicate that inhibition of the PI3K/Akt signaling pathway may lead to inhibition of the MDR induced by PrPC in gastric cancer cells The mechanism underlying PI3K/Akt- mediated ... PI3K/Akt is involved in PrPC-mediated MDR in gastric cancer In order to study whether activation of the PI3K/Akt signaling pathway played a role in PrPC-induced MDR in gastric cancer cells, the...

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