Application of bayesian model selection in fluorescence correlation spectroscopy (FCS) to WNT3EGFP secretion and diffusion in zebrafish embryos

... extended to the model determination of EGFP labeled proteins in living zebrafish embryos FCS has then been employed to investigate in vivo EGFP tagged Wnt3 (Wnt3EGFP) secretion and diffusion ... proteins remains problematic In this study, a Bayesian model selection approach has been applied to evaluate FCS data of fluorescent proteins in vitro and in vivo to address the issues of competing ... fitting to evaluated models 56 Figure 3.2 Bayesian analysis of Fluorescein with varying excitation intensity 57 Figure 3.3 Bayesian analysis of mixtures of Atto565 and Atto565-streptavidin...

Investigation of microfluidics in channels and tissues by fluorescence correlation spectroscopy (FCS)

... dynamics including diffusion, local concentration, and binding rate (67; 68) Scanning FCS Since traditional point FCS is more suitable for fast-diffusing molecules, in case of slow-diffusing or ... Fluorescence correlation microscopy (FCM), a technique term ﬁrst introduced in 1995 by Terry and coworkers (93) which combines imaging techniques with FCS, identiﬁed points of interest within a sample and ... determine protein dynamics and molecular interactions The use of two SPCMs in combination with a 50:50 non-polarizing beamsplitter allows the cross correlation measurement of SPCM signals eliminating...

INVESTIGATION OF MEMBRANE PROPERTIES IN THE CENTRAL NERVOUS SYSTEM OF DROSOPHILA MELANOGASTER STUDIED BY FLUORESCENCE CORRELATION SPECTROSCOPY

... Summary The objective of this study is to apply biophysical fluorescence technique, i.e fluorescence correlation spectroscopy (FCS), in situ in the central nervous system of fruit fly (Drosophila melanogaster) ... neuronal membrane fluidity was being studied in situ in the central nervous system of Drosophila melanogaster embryos by FCS Our approach promises to shed light on the biophysical features of cellular ... this is the first time confocal FCS is being used to study membrane fluidity in situ in the central nervous system of Drosophila melanogaster embryos A study by Sergent et al indicated that increased...

Investigation of peptide lipid interaction by fluorescence correlation spectroscopy

... and cell membrane organization investigated by imaging fluorescence cross‐ correlation spectroscopy. " Biophys J 97(9): 2630‐9. I Investigation of Peptide Lipid Interaction by Fluorescence Correlation Spectroscopy ... provides further guidelines for the rational design of antimicrobial peptides. VIII Investigation of Peptide Lipid Interaction by Fluorescence Correlation Spectroscopy List of Figures Fig 1.1 Schematic representation of the Gram‐negative bacteria cell wall. ... Phase of clinical trial Investigation of Peptide Lipid Interaction by Fluorescence Correlation Spectroscopy Table 1.1 Clinical developments of cationic antimicrobial peptides 14 Investigation of Peptide Lipid Interaction by Fluorescence Correlation Spectroscopy ...

Báo cáo khoa học: " Estimation of Paratuberculosis Prevalence in Dairy Cattle in a Province of Korea using an Enzyme-linked Immunosorbent Assay: Application of Bayesian Approach" ppt

... 748.2) Estimation of Paratuberculosis Prevalence in Dairy Cattle in a Province of Korea using an Enzyme-linked Immunosorbent Assay: Application of Bayesian Approach 53 the binomial distribution in ... Cattle in a Province of Korea using an Enzyme-linked Immunosorbent Assay: Application of Bayesian Approach 55 prevalence, as noted by Brenner and Gefeller [4] For sensitivity the resulting posteriors ... oseph, L and Ernst, P Bayesian estimation of asthma prevalence, and comparison of exercise and questionnaire diagnostics in the absence of a gold standard Ann Epidemiol 1998, 8(3), 201-208 Diamond,...

Báo cáo khoa học: "Interpreting the variations in xylem sap flux density within the trunk of maritime pine (Pinus pinaster Ait.): application of a model for calculating water flows at tree and stand levels" pdf

... semi-cylindrical shape, and calculating the sap flux as the average of the measurements made at a height of m on a sample of ten trees at Carrasqueira and at a height of 8.5 m on seven We trees at the ... on accurate determinations of the tree sap flow, which requires determination of sapwood area, mean sapflux density and needle area in a stand to a high degree of accuracy Thus, application of ... temperature and transpi- a crown ration rate; the resistance and capacitance of each compartment are independent of the flux or water potential of the compartment and remain constant during the day...

investigation on bayesian ying-yang learning for model selection in unsupervised learning

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Quantification of biomolecule dynamics and interactions in living zebrafish embryos by fluorescence correlation spectroscopy

... fiber cells. We finally analyze the FCS data with the anomalous subdiffusion model and study the molecular crowdedness of cells in living embryos. Chapter 5 describes the direct quantification of protein‐protein interactions in living zebrafish embryos with ... use in zebrafish embryos and demonstrate several applications of FCS in living zebrafish embryos, showing that single molecule‐based studies in living organism are possible: The autofluorescence ... foreign genes in zebrafish embryos are discussed and compared with that in cell cultures. Secondly, the autofluorescence in living zebrafish embryos, in particular the autofluorescence ...

Dynamics of online game industry in china an application of porters model

... Porter’s model in its application to study the case of China 29 Dynamics of online game industry in China Chp4 HISTORICAL OVERVIEW CHAPTER HISTORICAL OVERVIEW: ONLINE GAME INDUSTRY IN CHINA (2000 ... titles (January 2007) 88 II Dynamics of online game industry in China ABSTRACT ABSTRACT The Chinese online game industry is one of the fastest growing and dynamic interactive entertainment industries ... concentration of leading Chinese online game companies (2006) 80 B China s online game industry players and their relationships 81 C Origin of online game titles operated in China (2002...

Impact of regional trading blocs and free trading agreements on bilateral trade an application of gravity model in international trade

... pre-submission seminar and made valuable comments on the work being done, anonymous examiners and to Roshin, Ruwan, Nisantha, Gunasinghe and Pradeep for assisting me in data collection and proofreading ... Geopolitics of RTAs shows an increase of North-South RTAs Expansion and consolidation of regional integration schemes into Continentwide regional trading blocs 1.2 OBJECTIVES OF THE STUDY In this ... impact of FTA, trade creation (TC) and trade diversion (TD) effects of Regional Trading Blocs (RTBs) and the FTA and RTB interactive effects in promoting trade for member and non-member countries...

Tài liệu Application of Fuzzy Set Theory in Flexible Manufacturing System Design pptx

... Simulation of scheduling rules in a ﬂexible manufacturing system using fuzzy logic, IEA-AIE96 Ninth International Conference on Industrial and Engineering Application of Artiﬁcial Intelligence ... framework for the design of expert systems Indeed, the design of expert systems may well prove to be one of the most important applications of fuzzy logic in knowledge engineering and information technology ... 5 Application of Fuzzy Set Theory in Flexible Manufacturing System Design 5.1 5.2 A Kazerooni University of Lavisan K Abhary 5.3 5.4 5.5 University of South Australia L H S Luong University of...

Tài liệu Application of Knowledge Management Technology in Customer Relationship Management pptx

... acquiring or generating this knowledge by monitoring the activities of customers and other players in the industry The knowledge codiﬁcation & storage process involves converting knowledge into ... accessing necessary information, resolving inconsistencies in the Knowledge and Process Management retrieved information, ﬁltering away irrelevant and unwanted information, and integrating information ... corporations However, a single view of customers requires tightly integrated applications both within the KM Technology in Customer Relationship Management 15 RESEARCH ARTICLE realm of CRM applications and...

manual on the application of the haccp system in mycotoxin prevention and control pdf

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Báo cáo hóa học: " Review Article Multimodal Pressure-Flow Analysis: Application of Hilbert Huang Transform in Cerebral Blood Flow Regulation" docx

... we review previous applications of MMPF in clinical studies [15, 16], in which the ability and reliability of the method in assessing the CA from spontaneous BPBFV ﬂuctuations during resting ... “Endogenous circadian rhythm in an index of cardiac vulnerability independent of changes in behavior,” Proceedings of the National Academy of Sciences of the United States of America, vol 101, no ... 1998 [21] W Huang, Z Shen, N E Huang, and Y C Fung, “Engineering analysis of biological variables: an example of blood pressure over day,” Proceedings of the National Academy of Sciences of the United...

Báo cáo khoa học: "Optimization of multiple trait selection in western hemlock (Tsuga heterophylla (Raf.) Sarg.) including pulp and paper properties" pot

... influencing dry weight (DW) and tear strength of paper, but negatively influencing other pulp and paper characteristics (table II) Selection for volume would result in an increase in RW, CS and ... Cartwright C., Hatton J., Yanchuk A.D., The potential of improving western hemlock pulp and paper quality I Genetic control and interrelationships of wood and fibre traits, Can J For Res 28 (1998) 863–870 ... single and multiple objective optimization of selection; (d) to derive different alternatives for multiple selection indices involving different objective functions; (e) to present results, including...

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