... 5’-CATAGAAAGTGAACATTTAGGATGTG-3’ second PCR primer for sequence reaction 5’-CATGTGGCACCATCTCACAAT-3’ 5’GCTGCGAGCTCACCCAGAATGTCTGG-3’ 62°C 5’-GAATTCGGATGCAGAGCTTCTT-3’ 5’-Biotin-CCCACA CAGCAA AGCAGAAACT-3’ ... Cite this article as: Dufort et al.: Pyrosequencing, a method approved to detect the two major EGFR mutations for anti EGFR therapy in NSCLC Journal of Experimental & Clinical Cancer Research 2011 ... Clin Oncol 2011 Tanaka T, Matsuoka M, Sutani A, Gemma A, Maemondo M, Inoue A, Okinaga S, Nagashima M, Oizumi S, Uematsu K, Nagai Y, Moriyama G, Miyazawa H, Ikebuchi K, Morita S, Kobayashi K, Hagiwara...