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Characterization and deactivation of sulfided red mud used as hydrogenation catalyst

Tài liệu Báo cáo khoa học: Preliminary molecular characterization and crystallization of mitochondrial respiratory complex II from porcine heart ppt

Tài liệu Báo cáo khoa học: Preliminary molecular characterization and crystallization of mitochondrial respiratory complex II from porcine heart ppt

... slaughterhouse was shearedinto pieces and immediately immersed in liquid nitrogen.One hundred milligrams of heart tissue was picked up and milled into powder in liquid nitrogen, which was then used to ... n-de-cyl-b-d-maltoside was selected as the detergent for cry-stallization, and n-nonyl-b-d-maltoside as the additive.Another important factor for crystallization of com-plex II is sucrose. Sucrose was used as ... fullyreleased at the time of our study, we measured theaccurate mature amino acid sequences of the fourcomplex II subunits by RT-PCR and N-terminalsequencing. The nucleotide sequences of the...
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Tài liệu Báo cáo khoa học: Physico-chemical characterization and synthesis of neuronally active a-conotoxins docx

Tài liệu Báo cáo khoa học: Physico-chemical characterization and synthesis of neuronally active a-conotoxins docx

... the diversity and utility of the pool of available a-conotoxins. Their identificationandcharacterizationdependonasuiteoftechniqueswith increasing emphasis on mass spectrometry and micro-scale ... conopeptideanalysis and characterization is by no means trivial, and often optimization on a case-by-case basis is required [14].Sample amounts may be limiting even with the enhancedsensitivity of current ... modification, and differentiation of sulfotyrosine and phosphotyrosine [38]. Characterization of the sulfotyrosine-containing a-cono-toxin EpI was undertaken by a combination of massspectrometry and...
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Tài liệu Báo cáo khoa học: Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima doc

Tài liệu Báo cáo khoa học: Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima doc

... groups, and depolymerases. The latter can beclassified into lyases (b-elimination) and hydrolases [2].All hydrolases involved in degradation of pectin areclassified as members of family 28 of the ... glycosidehydrolases, including the endopolygalacturonases, exo-polygalacturonases and rhamnogalacturonases [3,4].Although a handful of endopolygalacturonases, gener-ally of fungal origin [5–10], and ... presence of at least two pec-tinase-encoding genes [25]. One of these, PelA, hasbeen characterized in detail as an extracellular exopec-tate lyase, releasing unsaturated trigalacturonate as themajor...
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Tài liệu Báo cáo Y học: Characterization and regulation of yeast Ca2+-dependent phosphatidylethanolamine-phospholipase D activity docx

Tài liệu Báo cáo Y học: Characterization and regulation of yeast Ca2+-dependent phosphatidylethanolamine-phospholipase D activity docx

... activity was measured with the indicated free Ca2+concentrations. The amount of cytosolic protein included in the assaywas 32 lg per reaction and the amount of membrane protein was0.4 lg ... pattern and extent of stimulation of the membrane and soluble yeast PtdEtn-PLD activity by Ca2+ and their inhibition by other divalentcations is highly comparable.The mechanism of action of Ca2+ions ... cytosolic and membrane-bound PtdEtn-PLD activity. Cytosolic and membrane-bound fractionswere prepared as described in Materials and methods. PtdEtn-PLDactivity measured without addition of EDTA,...
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Characterization and Authentication of Olive and Other Vegetable Oils pptx

Characterization and Authentication of Olive and Other Vegetable Oils pptx

... and MLR Models . . . . . 2059.5 Prediction of OFA Concentration in VOOs Using FTIRandMLR 2089.5.1 Description of FTIR Spectra and Construction of Data Matrices and MLR Models . . . . . . . . ... comprising 0.2 and 1.3 % of total fatty acids, respectively. Their present in an olive oil is an index of lowquality (Mariani and Fedeli 1985). For this reason, their determination is often used as an ... to the protection against chronic and degenerative diseases such as heartdisease, diseases of neuro-degenerative aging and tumors located in various parts of the human body (Franceschi 1999;...
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Báo cáo khoa học: Enzymatic toxins from snake venom: structural characterization and mechanism of catalysis ppt

Báo cáo khoa học: Enzymatic toxins from snake venom: structural characterization and mechanism of catalysis ppt

... metal-loproteinases, acetylcholinesterases (AChEs), l-aminoacid oxidases, nucleotidases (5¢-nucleotidases, ATPases,phosphodiesterases and DNases) and hyaluronidases.In most cases, snake venoms ... acetylcholinesterase, as well as doing theoverall compilation and formatting of the manuscript.AV and ST prepared the sections on l-amino acid oxi-dase and snake venom metalloproteinase respectively.MTM and ... formedbetween the Nd1-H of His57 and Od1 of Asp102, as well as between the OH of Ser195 and the Ne2-H of His57. The hydrogen bond between the latter pairis disrupted upon protonation of His57. Recent...
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Báo cáo khoa học: Characterization and regulation of a bacterial sugar phosphatase of the haloalkanoate dehalogenase ppt

Báo cáo khoa học: Characterization and regulation of a bacterial sugar phosphatase of the haloalkanoate dehalogenase ppt

... adding 1 mL of 0.2 m NaOH,the tubes were centrifuged at 16 000 g for 1 min and 1 mL of the supernatant was recovered for measurement of A405.A calibration curve for phosphatase activity assays ... two subclasses: subclass IIA and subclass IIB [10].Presently,  2000 sequences are assigned to HADsubfamily IIA, which covers humans and other eukary-otes, as well as Gram-positive and Gram-negative ... +4007, was inserted intothe vector pAC5 (Table 1), yielding pLG25. PlasmidpLG25 carries a translational fusion between codon 10 of araL and codon 7 of E. coli lacZ. pLG25 was used as template...
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Báo cáo Y học: Synthesis, characterization and application of two nucleoside triphosphate analogues, GTPcNH2 and GTPcF pdf

Báo cáo Y học: Synthesis, characterization and application of two nucleoside triphosphate analogues, GTPcNH2 and GTPcF pdf

... investigated. The rate of hydrolysis of GTPcNH2bound to Ras protein lay betweenthe rates found for Ras-bound GTPcS and GppNHp, whileRas-catalysed hydrolysis of GTPcF was almost as fast as forGTP. The ... spectroscopic and structuralstudies was tested by formation of the complex betweenRasGTPcNH2 and the Ras-binding domain of the Raseffector protein c-Raf-1.MATERIALS AND METHODSGeneral description of ... (residues1–167) was synthesized in Escherichia coli and purified as described [16]. Purified GDP, GTP, GTPcS and GppNHpreagents were purchased from Sigma and GppCH2pwasordered from JenaBioScience....
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Báo cáo khoa học: Mycobacterium tuberculosis ClpC1 Characterization and role of the N-terminal domain in its function ppt

Báo cáo khoa học: Mycobacterium tuberculosis ClpC1 Characterization and role of the N-terminal domain in its function ppt

... Effect of divalent metal ions on the ATPase activity of M. tuberculosis ClpC1 and its deletion mutants. ATPase activity of proteins was assayed as described and effect of various divalentions was ... aggregation of luciferase (Fig. 6C).There was some aggregation of ClpC1 and ClpC1D2inthe presence of ATP at 43 °C (Fig. 6D). However, avery rapid and high aggregation of ClpC1D1 with ATPwas observed ... was recovered. Although ClpC1D1 wasfound to not prevent aggregation it was able to reac-tivate luciferase, however, it had a reduced activitycompared with ClpC1 and ClpC1D2 (Fig. 7). BSA wasnot...
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