... Coenzyme A affects firefly luciferase luminescence because it acts as a substrate and not as an allosteric effector Hugo Fraga1,2, Diogo Fernandes1,2, Rui Fontes2 and Joaquim C. G. ... structural similarity between firefly luciferase and acyl-CoA synthetases, our achievement is not as strange as it seems to be and supports the theory thatnowadays firefly luciferase evolved from an ancestralacyl-CoA ... 95,194–198.30 Zako T, Ayabe K, Aburatani T, Kamiya N,Kitayama A, Ueda H & Nagamune T (2003)Luminescent and substrate binding activities of firefly luciferase N-terminal domain. Biochim Biophys Acta1649,...