... to be viable and uncontaminated To assure viability and sterility, a vial was recovered from the freezer and passaged times and then tested for mycoplasmal, bacterial and fungal contaminants Six ... cultured from venous ulcers display cellular characteristics of senescence J Vasc Surg 1998, 28:876-883 Hasan A, Murata H, Falabella A, Ochoa S, Zhou L, Badiavas E, Falanga V: Dermal fibroblasts from ... μg/ml) and Gentamicin (50 μg/ml) was used The flask was inverted and ml additional medium was added This facilitated the rapid attachment of the cells from the biopsy to the flask After at least...