... N-terminal region of the titin located between the Z-band and the N2-line in the I-band of myofibrils. This titin region,rich in PEST sequences, was reported to show a markedcalcium binding ability ... the two subunits in calpains[18]. Indeed, the two subunits of 80 kDa (domains I–IV) and 30 kDa (domains V–VI), contain altogether 10–11putative EF-hands motifs in domains IV, VI and II[1,19,20], ... domains and EF-hand motifs. Finally, itallows dissociation of two aspects in the protease behaviourtoward its target, binding and cleavage, in relation to thepresence of Ca2+ions.Materials and...