... (CCTCAGAACGTTGATGGCA)and P2r (ATTGCTTTCCTTTTTCACAA GA) and allele-specific primer s Pnf (AGCATTTGGTTTTAAATTATG-GAGTATATG) and Pmr (GTTTTACTTACTCTCGTCTCCACAAAA). The PCR was run for 35 cycles ... K, Kameda T, Takenaka K, Oku S,Abe H, Katayose KS, Kubuki Y, Kusumoto K, Hasuike S, Tahara Y, Nagata K,Matsuda T, Ohshima K, Harada M, Shimoda K: Development of ET, primarymyelofibrosis and ... with JAK2V617F, it may take many years to show a clear MPN symptom, and some may never reach the stagebefore they die of other diseases. It is also likely thatJAK2V617F-bearing cells may stay dormant...